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The Transgenic Animal and Genome Editing Core (CCHMC) operates under the direction of Yueh-Chiang Hu, PhD, and provides streamlined service from DNA vector to founder animals.
This facility uses the latest genome-editing technologies, such as CRISPR-Cas9 and TALEN, to generate animals carrying multiple knockout or knock-in alleles in a highly efficient and time-saving fashion. This facility also uses conventional approaches
to generate transgenic mice by pronuclear microinjection and chimeric mice using embryonic stem cells. Other services available in the facility include cell targeting, targeting vector construction, sperm and embryo cryopreservation, BAC transgenics,
mouse recovery from cryopreserved sperm, intra-cytoplasmic sperm injection (ICSI), and embryo transfer (re-derivation). The facility has also undertaken several new initiatives including the implementation of a fast CRISPR-Cas9 genetic screen system
and generation of genetically modified rats. The facility provides consultation services and instruction in basic molecular biology techniques to researchers and trainees beginning to use these tools.
Research supported by the Center for Environmental Genetics should cite NIEHS award P30-ES006096
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