NMR-based Metabolomics Core
The NMR-Based Metabolomics Core (NBMC) at CCHMC provides state-of-the-art technology in a centralized location accessible to research investigators and clinicians, serving Cincinnati Children’s and University of Cincinnati College of Medicine (UC COM) communities. We strive to foster collaborative efforts that will advance translational research using metabolomics approaches. We hope to facilitate institutional development of pioneering research, metabolomics training and outreach.
The NBMC facilitates broad spectrum and targeted metabolomics analysis of polar components, as well as methods for targeted analysis of metabolites, with experience in the analysis of cells, organ tissue (e.g. liver, muscle, intestines, tongue, and tumor), biological fluids (e.g. urine, serum, plasma, amniotic fluid and saliva), and exhaled breath collected from human subjects or animal models.
The Metabolomics Facility is fully equipped to study multiple aspects of NMR, including 1H, 13C, and two-dimensional studies. We have performed expanded quantitative metabolic analysis on the following:
- Cells extracts/Cell secretions (media)
- Human and animal tissues and biopsy extracts
- Body fluids (blood, plasma, serum, urine, amniotic fluid, saliva, etc.)
The NBMC facility, located within the Division of Pathology at Cincinnati Children’s, utilizes a Bruker IVDr 600MHz NMR system that is fully-automated, designed specifically for metabolomics based studies of biological fluids, housed in the Medical Science Building at UC COM. The primary spectrometer utilized by the NBMC provides a high field multi-nuclear NMR capability to Cincinnati Children's Hospital Medical Center, UCCOM and external academic and industry collaborators. The Bruker IVDr, a top-of-the-line spectrometer, is capable of running most contemporary homonuclear, heteronuclear one and two-dimensional pulse sequences, using pre-designed software, and is equipped with a 96-well SampleJet auto-sampler unit that renders the NMR system fully-automated. The Bruker spectrometer is equipped with a 5mm triple resonance conventional probe, which allows for routine acquisition of 1D and 2D 1H-1H correlations as well as 1D 13C and 2D 1H-13C correlations used to confirm small metabolite identities.