micro-CT ex-vivo embryo

rat embryo soaked in Lugol's

Rat PET CT

F-18 FDG PET-CT for monitoring glioma treatment response. 10 mins PET acquisition at 30 mins post injection, following CT scan

micro-CT in-vivo gated lung scan

Respiratory-gated CT for Pulmonary Alveolar Proteinosis (PAP) Mouse under isoflurane.

X ray

Bone Densitometry

Bioluminescence (BLI) imaging

Tumor imaging (breast cancer model) 20min acquisition following IP luciferin injection

Fluorescence (FLR) imaging

Tumor imaging using antibody with Alexa-647 fluorescent label. 30sec acquisition at 24hr after FLR injection.

Irradiation of cell plates

Focal Irradiation - example set-up

Inveon PET/SPECT/CT

Tri-modal micro-imaging system with LSO-based positron emission tomography (micro-PET), dual-head single photon emission computed tomography (micro-SPECT), and cone-beam computerized tomography (micro-CT) sub-systems.
a) Bore size = 12 cm; max FOV = 10cm.

Integrated Biovet monitoring system: respiratory, EKG, temperature control; heating pad on pallet
Isoflurane anesthesia

Siemens

Bruker multi-spectral FX PRO

Bioluminescence, fluorescence, x-ray, and optical imaging in small animals.

a) 20 - 200 mm FOV (images up to 5 mice simultaneously)
b) warm-air chamber with isoflurane anesthesia manifolds
c) excitation filters: 390nm to 770nm in 20nm steps 
d) emission filters: 535nm, 600 nm, 700nm, 750nm, 790nm, 830nm
 

Bruker

Xen-X Irradiator

Closed cabinet x-ray irradiator with a rotational gantry, designed for irradiation of cells and rodents. Provides both whole-body irradiation for myelosuppression, and focal irradiation similar to current clinical radiotherapy approaches.  A portal imaging camera provides verification of the beam localization.
 

• (https://xstrahl.com/life-science-systems/xenx/)
• 50 – 220 kV x-rays
• Mounted on isocentric arm with 35 cm source-to-isocenter distance
• 3 mm round to 10 cm x 10 cm fields at standard distance, larger at extended distances
• Point dose calculator
• 0.69 mm Cu HVL treatment x-rays

X-Strahl

What is the lab phone number?

513 558 7930

How do I arrange to transfer my animals for imaging?

All live animal imaging is conducted under the Core's IACUC protocol.
A "Temporary Transfer" must be submitted through UC LAMS to protocol Lemen #10-01-13-01.  Please contact the lab for details and assistance.

How do I schedule imaging time?

Please call the lab - 558-7930 - for available times and to plan for longitudinal imaging timepoints.

Which animals may be imaged in the lab?

All core equipment is optimized for imaging rodents - mice and rats. 
The core is a Specific Pathogen Free facility. Animals within UC facilities may be imaged or irradiated here. For transfers from outside facilites, please contact the lab to confirm if they will be accepted.  

Please notify the facility of assisted publications and grants. Acknowledgement of our services helps to ensure the continuation of the imaging core services.

Ravi N. Samy; Brian R. Earl; Noga Lipschitz; Ivy Schweinzger; Mark Currier; Timothy Cripe, "Engineered Oncolytic Virus for the Treatment of Cholesteatoma: A Pilot in vivo Study" Laryngoscope Investigative Otolaryngology, 4: October 2019: 532-542

Sumit Murab, Stacey M.S. Gruber, Chia-Ying James Lin, Patrick Whitlock, "Elucidation of bio-inspired hydroxyapatie crystallization on oxygen-plasma modified 3D printed poly-caprolactone scaffolds", Materials Science & Engineering C 109 (2020) 110529

in-vivo CT articles

ex-vivo CT articles

micro-CT - general

Adeola Adeyemo, Christopher Johnson, Andrew Stiene, Kathleen LaSance, Zhihua Qi, Lisa Lemen & Jo El J. Schultz (2020) Limb functional recovery is impaired in fibroblast growth factor-2 (FGF2) deficient mice despite chronic ischaemia-induced vascular growth, Growth Factors, 38:2, 75-93, DOI: 10.1080/08977194.2020.1767612