College of Medicine Research Core Facilities
The UC College of Medicine houses a number of research core facilities designated as core service centers. These facilities exist within multiple departments but are collectively supported by the College of Medicine, Office of Research through the Associate Dean for Research Core Facilities: Ken Greis, PhD. (firstname.lastname@example.org; Tel: 515-558-7012).
The service center designation signifies that the rates charged by each of these facilities have been reviewed and approved by the UC government cost compliance office, thus the service fees can be cross-charged to federal grants and contracts. Details related to the services offered and the internal rates for each of the cores are provided below. Since these rates are substantially subsidized by the University, external investigators should contact individual core directors to get a rate quote.
Resources to offset some of the cost of the core services may be available through a variety of centers and institutes across UC depending on an investigator’s affiliation. Information related to some of those opportunities and the website links are provided below:
UC Flow Cytometry Core
The Flow Core supports a Beckman CytoFLEX S with 9 detectors and 4 lasers in CARE 2874 and a FACSCalibur 4 color flow cytometer system in CARE 2876. Access is available 24 hrs a day and 7 days per week once investigators are registered in Stratocore and approved by the core director.
For access to the instruments and the online calendar, please contact Dr. Miller.
CytoFLEX S System Access (4 laser, 9 color)
||$20 per half hour|
FACSCalibur System access (2 Laser, 4 color)
||$20 per half hour|
FACSCalibur Four Color Flow Cytometer
The FACSCalibur is configured as follows:
- FL1 Channel - Blue Laser (488nm) and 515-545nm Emission Filter (FITC, GFP, etc.)
- FL2 Channel - Blue Laser (488nm) and 564-601nm Emission Filter (PE, PI, RFP, etc.)
- FL3 Channel - Blue Laser (488nm) and 670LP Emission Filter (7-AAD, PerCP, etc)
- FL4 Channel - Red Laser (635nm) and 653-669nm Emission Filter (APC, Cy5, etc.)
Beckman CytoFLEX S V2-B2-Y3-R2 Flow Cytometer (9 Detectors, 4 Lasers)
Here are the channels currently configured (in bold) the ones in italics can be utilized by repositioning the filters, which is relatively easy to do.
CytoFLEX (4 laser, 9 color)
Violet Laser (405nm) Emmision Filter
PB450 (PacBlue, eFluor450) 450/45
KO525 (Krome Orange, Zombie Aqua) 525/40
Blue Laser (488nm)
FITC (GFP, Alexa488, Venus) 525/40
PerCP (PC5, PC5.5) 690/50
Yellow/Green Laser (561nm)
PE (PI, Sytox Orange, DsRed, TdTomato) 585/42
ECD (mCherry, Texas Red) 610/20
Red Laser (638nm)
APC (AlexaFluor 647, eFluor660) 660/10
APC-A750 (APC-Cy7, APC eFluor780 780/60
APC-A700 (AlexaFluor 700)
213 Albert Sabin Way
- William Miller PhD