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Photo of  David Askew, PhD

David Askew, PhD

Research Interests

Aspergillus fumigatus is an environmental fungus that causes life-threatening pulmonary infections that have a very high rate of mortality.  The organism is a potent opportunistic pathogen that takes advantage of a weakened immune system to...

Education/Credentials
  • PhD: University of British Columbia
  • B.Sc.: University of British Columbia
Contact Information
  • Office 1256A Medical Sciences Building
  • 231 Albert Sabin Way
  • Cincinnati, Ohio 45267-0529
  • Office (513) 558-2395
  • Fax (513) 558-2289
  • Email david.askew@uc.edu

Peer Reviewed Publications

Raj, Shriya; Krishnan, Karthik; Askew, David S; Helynck, Olivier; Suzanne, Peggy; Lesnard, Aurélien; Rault, Sylvain; Zeidler, Ute; d'Enfert, Christophe; Latgé, Jean-Paul; Munier-Lehmann, Hélène; Saveanu, Cosmin 2016. The Toxicity of a Novel Antifungal Compound Is Modulated by Endoplasmic Reticulum-Associated Protein Degradation Components. Antimicrobial agents and chemotherapy, 60 3, 1438-49

Krishnan, Karthik; Ren, Zhaowei; Losada, Liliana; Nierman, William C; Lu, Long Jason; Askew, David S 2014. Polysome profiling reveals broad translatome remodeling during endoplasmic reticulum (ER) stress in the pathogenic fungus Aspergillus fumigatus. BMC genomics, 15 , 159

Jambunathan, Kalyani; Watson, Douglas S; Najvar, Laura K; Wiederhold, Nathan P; Kirkpatrick, William R; Patterson, Thomas F; Askew, David S; Kodukula, Krishna; Galande, Amit K 2013. Prolyl endopeptidase activity in bronchoalveolar lavage fluid: a novel diagnostic biomarker in a guinea pig model of invasive pulmonary aspergillosis. Medical mycology, 51 6, 592-602

Krishnan, Karthik; Feng, Xizhi; Powers-Fletcher, Margaret V; Bick, Gregory; Richie, Daryl L; Woollett, Laura A; Askew, David S 2013. Effects of a defective endoplasmic reticulum-associated degradation pathway on the stress response, virulence, and antifungal drug susceptibility of the mold pathogen Aspergillus fumigatus. Eukaryotic cell, 12 4, 512-9

Powers-Fletcher, M. V.; Feng, X.; Krishnan, K.; Askew, D. S. 2013. Deletion of the sec4 Homolog srgA from Aspergillus fumigatus Is Associated with an Impaired Stress Response, Attenuated Virulence and Phenotypic Heterogeneity PLoS One, 8 6 , e66741

Powers-Fletcher, Margaret V; Feng, Xizhi; Krishnan, Karthik; Askew, David S 2013. Deletion of the sec4 homolog srgA from Aspergillus fumigatus is associated with an impaired stress response, attenuated virulence and phenotypic heterogeneity. PloS one, 8 6, e66741

Gravelat, F. N.; Askew, D. S.; Sheppard, D. C. 2012. Targeted gene deletion in Aspergillus fumigatus using the hygromycin-resistance split-marker approach Methods Mol Biol, 845 4 , 119-30

Feng, X.; Krishnan, K.; Richie, D. L.; Aimanianda, V.; Hartl, L.; Grahl, N.; Powers-Fletcher, M. V.; Zhang, M.; Fuller, K. K.; Nierman, W. C.; Lu, L. J.; Latge, J. P.; Woollett, L.; Newman, S. L.; Cramer, R. A., Jr.; Rhodes, J. C.; Askew, D. S. 2011. HacA-independent functions of the ER stress sensor IreA synergize with the canonical UPR to influence virulence traits in Aspergillus fumigatus PLoS Pathog, 7 10 , e1002330

Fuller, Kevin K; Richie, Daryl L; Feng, Xizhi; Krishnan, Karthik; Stephens, Timothy J; Wikenheiser-Brokamp, Kathryn A; Askew, David S; Rhodes, Judith C 2011. Divergent Protein Kinase A isoforms co-ordinately regulate conidial germination, carbohydrate metabolism and virulence in Aspergillus fumigatus. Molecular microbiology, 79 4, 1045-62

Powers-Fletcher, Margaret V; Jambunathan, Kalyani; Brewer, Jordan L; Krishnan, Karthik; Feng, Xizhi; Galande, Amit K; Askew, David S 2011. Impact of the lectin chaperone calnexin on the stress response, virulence and proteolytic secretome of the fungal pathogen Aspergillus fumigatus. PloS one, 6 12, e28865

Richie, D. L.; Feng, X.; Hartl, L.; Aimanianda, V.; Krishnan, K.; Powers-Fletcher, M. V.; Watson, D. S.; Galande, A. K.; White, S. M.; Willett, T.; Latge, J. P.; Rhodes, J. C.; Askew, D. S. 2011. The virulence of the opportunistic fungal pathogen Aspergillus fumigatus requires cooperation between the endoplasmic reticulum-associated degradation pathway (ERAD) and the unfolded protein response (UPR) Virulence, 2 1 , 12-21

Richie, D. L.; Feng, X.; Krishnan, K.; Askew, D. S. 2011. Secretion stress and antifungal resistance: an Achilles' heel of Aspergillus fumigatus? Med Mycol, 49 Suppl 1 10 , S101-6

Richie, Daryl L; Feng, Xizhi; Hartl, Lukas; Aimanianda, Vishukumar; Krishnan, Karthik; Powers-Fletcher, Margaret V; Watson, Douglas S; Galande, Amit K; White, Stephanie M; Willett, Taryn; Latgé, Jean-Paul; Rhodes, Judith C; Askew, David S 2011. The virulence of the opportunistic fungal pathogen Aspergillus fumigatus requires cooperation between the endoplasmic reticulum-associated degradation pathway (ERAD) and the unfolded protein response (UPR). Virulence, 2 1, 12-21

Watson, Douglas S; Feng, Xizhi; Askew, David S; Jambunathan, Kalyani; Kodukula, Krishna; Galande, Amit K 2011. Substrate specifity profiling of the Aspergillus fumigatus proteolytic secretome reveals consensus motifs with predominance of Ile/Leu and Phe/Tyr. PloS one, 6 6, e21001

Watson, Douglas S; Jambunathan, Kalyani; Askew, David S; Kodukula, Krishna; Galande, Amit K 2011. Robust substrate profiling method reveals striking differences in specificities of serum and lung fluid proteases. BioTechniques, 51 2, 95-104

Park, S. J.; Burdick, M. D.; Brix, W. K.; Stoler, M. H.; Askew, D. S.; Strieter, R. M.; Mehrad, B. 2010. Neutropenia enhances lung dendritic cell recruitment in response to Aspergillus via a cytokine-to-chemokine amplification loop J Immunol, 185 10 , 6190-7

Fuller, Kevin K; Zhao, Wei; Askew, David S; Rhodes, Judith C 2009. Deletion of the protein kinase A regulatory subunit leads to deregulation of mitochondrial activation and nuclear duplication in Aspergillus fumigatus. Eukaryotic cell, 8 3, 271-7

Richie, Daryl L; Hartl, Lukas; Aimanianda, Vishukumar; Winters, Michael S; Fuller, Kevin K; Miley, Michael D; White, Stephanie; McCarthy, Jason W; Latgé, Jean-Paul; Feldmesser, Marta; Rhodes, Judith C; Askew, David S 2009. A role for the unfolded protein response (UPR) in virulence and antifungal susceptibility in Aspergillus fumigatus. PLoS pathogens, 5 1, e1000258

Askew, D. S. 2008. Aspergillus fumigatus: virulence genes in a street-smart mold Curr Opin Microbiol, 11 4 , 331-7

Bhabhra, Ruchi; Richie, Daryl L; Kim, H Stanley; Nierman, William C; Fortwendel, Jarrod; Aris, John P; Rhodes, Judith C; Askew, David S 2008. Impaired ribosome biogenesis disrupts the integration between morphogenesis and nuclear duplication during the germination of Aspergillus fumigatus. Eukaryotic cell, 7 4, 575-83

Fortwendel, Jarrod R; Fuller, Kevin K; Stephens, Timothy J; Bacon, W Clark; Askew, David S; Rhodes, Judith C 2008. Aspergillus fumigatus RasA regulates asexual development and cell wall integrity. Eukaryotic cell, 7 9, 1530-9

Richie, D. L.; Askew, D. S. 2008. Autophagy in the filamentous fungus Aspergillus fumigatus Methods Enzymol, 451 10 , 241-50

Richie, Daryl L; Fuller, Kevin K; Fortwendel, Jarrod; Miley, Michael D; McCarthy, Jason W; Feldmesser, Marta; Rhodes, Judith C; Askew, David S 2007. Unexpected link between metal ion deficiency and autophagy in Aspergillus fumigatus. Eukaryotic cell, 6 12, 2437-47

Richie, Daryl L; Miley, Michael D; Bhabhra, Ruchi; Robson, Geoffrey D; Rhodes, Judith C; Askew, David S 2007. The Aspergillus fumigatus metacaspases CasA and CasB facilitate growth under conditions of endoplasmic reticulum stress. Molecular microbiology, 63 2, 591-604

Bhabhra, Ruchi; Zhao, Wei; Rhodes, Judith C; Askew, David S 2006. Nucleolar localization of Aspergillus fumigatus CgrA is temperature-dependent. Fungal genetics and biology : FG & B, 43 1, 1-7

Zhao, Wei; Panepinto, John C; Fortwendel, Jarrod R; Fox, Lauren; Oliver, Brian G; Askew, David S; Rhodes, Judith C 2006. Deletion of the regulatory subunit of protein kinase A in Aspergillus fumigatus alters morphology, sensitivity to oxidative damage, and virulence. Infection and immunity, 74 8, 4865-74

Bhabhra, R.; Askew, D. S. 2005. Thermotolerance and virulence of Aspergillus fumigatus: role of the fungal nucleolus Med Mycol, 43 Suppl 1 1 , S87-93

Fortwendel, Jarrod R; Zhao, Wei; Bhabhra, Ruchi; Park, Steven; Perlin, David S; Askew, David S; Rhodes, Judith C 2005. A fungus-specific ras homolog contributes to the hyphal growth and virulence of Aspergillus fumigatus. Eukaryotic cell, 4 12, 1982-9

Vogt, Keith; Bhabhra, Ruchi; Rhodes, Judith C; Askew, David S 2005. Doxycycline-regulated gene expression in the opportunistic fungal pathogen Aspergillus fumigatus. BMC microbiology, 5 , 1

Bhabhra, Ruchi; Miley, Michael D; Mylonakis, Eleftherios; Boettner, Doug; Fortwendel, Jarrod; Panepinto, John C; Postow, Michael; Rhodes, Judith C; Askew, David S 2004. Disruption of the Aspergillus fumigatus gene encoding nucleolar protein CgrA impairs thermotolerant growth and reduces virulence. Infection and immunity, 72 8, 4731-40

Fortwendel, Jarrod R; Panepinto, John C; Seitz, Amy E; Askew, David S; Rhodes, Judith C 2004. Aspergillus fumigatus rasA and rasB regulate the timing and morphology of asexual development. Fungal genetics and biology : FG & B, 41 2, 129-39

Panepinto, John C; Oliver, Brian G; Fortwendel, Jarrod R; Smith, Darcey L H; Askew, David S; Rhodes, Judith C 2003. Deletion of the Aspergillus fumigatus gene encoding the Ras-related protein RhbA reduces virulence in a model of Invasive pulmonary aspergillosis. Infection and immunity, 71 5, 2819-26

Moy, Terence I; Boettner, Douglas; Rhodes, Judith C; Silver, Pamela A; Askew, David S 2002. Identification of a role for Saccharomyces cerevisiae Cgr1p in pre-rRNA processing and 60S ribosome subunit synthesis. Microbiology (Reading, England), 148 Pt 4, 1081-90

Oliver, Brian G; Panepinto, John C; Askew, David S; Rhodes, Judith C 2002. cAMP alteration of growth rate of Aspergillus fumigatus and Aspergillus niger is carbon-source dependent. Microbiology (Reading, England), 148 Pt 8, 2627-33

Oliver, Brian G; Panepinto, John C; Fortwendel, Jarrod R; Smith, Darcey L; Askew, David S; Rhodes, Judith C 2002. Cloning and expression of pkaC and pkaR, the genes encoding the cAMP-dependent protein kinase of Aspergillus fumigatus. Mycopathologia, 154 2, 85-91

Panepinto, John C; Oliver, Brian G; Amlung, Thomas W; Askew, David S; Rhodes, Judith C 2002. Expression of the Aspergillus fumigatus rheb homologue, rhbA, is induced by nitrogen starvation. Fungal genetics and biology : FG & B, 36 3, 207-14

Boettner, D.; Huebner, N.; Rhodes, J. C.; Askew, D. S. 2001. Molecular cloning of Aspergillus fumigatus CgrA, the ortholog of a conserved fungal nucleolar protein Med Mycol, 39 6 , 517-21

Rhodes, J. C.; Oliver, B. G.; Askew, D. S.; Amlung, T. W. 2001. Identification of genes of Aspergillus fumigatus up-regulated during growth on endothelial cells Med Mycol, 39 3 , 253-60

Sun, J; Boettner, D; Huebner, N; Xu, F; Rhodes, J C; Askew, D S 2001. Molecular cloning of cgrA, the gene encoding the Aspergillus nidulans ortholog of Saccharomyces cerevisiae CGR1. Current microbiology, 42 6, 403-7

Sun, J; McFarland, M; Boettner, D; Panepinto, J; Rhodes, J C; Askew, D S 2001. Cgr1p, a novel nucleolar protein encoded by Saccharomyces cerevisiae orf YGL0292w. Current microbiology, 42 1, 65-9

Sun, J.; Rhodes, J. C.; Askew, D. S. 1998. Sequencing of a gene encoding a member of the mitochondrial carrier family of transport proteins from Aspergillus nidulans DNA Seq, 9 1 , 1-8

Li, J; Witte, D P; Van Dyke, T; Askew, D S 1997. Expression of the putative proto-oncogene His-1 in normal and neoplastic tissues. The American journal of pathology, 150 4, 1297-305

Askew, D S; Li, J; Ihle, J N 1994. Retroviral insertions in the murine His-1 locus activate the expression of a novel RNA that lacks an extensive open reading frame. Molecular and cellular biology, 14 3, 1743-51

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