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Department of Internal Medicine

Hematology & Oncology

Photo of  Atsuo Sasaki, PhD

Atsuo Sasaki, PhD

Associate Professor of Medicine Cancer Biology

Education/Credentials
  • Doctoral Degree: Kurume University School of Medicine
  • Master's of Science Degree: Hiroshima University
  • Bachelor's Degree: Tokyo University of Science

Research/Clinical Interests

The GTP-Sasaki Lab is investigating the role of GTP metabolism in human diseases. We are GTP geeks and we recently discovered the very first GTP-energy sensor kinase, as recently published inMolecular Cell. Much more high-impact work remains to be done on GTP sensor kinase and GTP-metabolism. The Sasaki Lab investigates GTP for Life with goals: To understand how cells Respond and Regulate GTP-energy levels; To apply our findings for developing new therapeutics for brain tumors and better life.

Contact Information
  • Vontz Center for Molecular Studies
  • 3125 Eden Avenue, Room 2112
  • ML 0508
  • Cincinnati, Ohio 45267
  • Office 513-558-5611
  • Fax 513-558-6703
  • Email atsuo.sasaki@uc.edu

Peer Reviewed Publications

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Kofuji, Satoshi; Hirayama, Akiyoshi; Eberhardt, Alexander Otto; Kawaguchi, Risa; Sugiura, Yuki; Sampetrean, Oltea; Ikeda, Yoshiki; Warren, Mikako; Sakamoto, Naoya; Kitahara, Shuji; Yoshino, Hirofumi; Yamashita, Daisuke; Sumita, Kazutaka; Wolfe, Kara; Lange, Lisa; Ikeda, Satsuki; Shimada, Hiroko; Minami, Noriaki; Malhotra, Akshiv; Morioka, Shin; Ban, Yuki; Asano, Maya; Flanary, Victoria L; Ramkissoon, Annmarie; Chow, Lionel M L; Kiyokawa, Juri; Mashimo, Tomoyuki; Lucey, Greg; Mareninov, Sergey; Ozawa, Tatsuya; Onishi, Nobuyuki; Okumura, Koichi; Terakawa, Jumpei; Daikoku, Takiko; Wise-Draper, Trisha; Majd, Nazanin; Kofuji, Kaori; Sasaki, Mika; Mori, Masaru; Kanemura, Yonehiro; Smith, Eric P; Anastasiou, Dimitrios; Wakimoto, Hiroaki; Holland, Eric C; Yong, William H; Horbinski, Craig; Nakano, Ichiro; DeBerardinis, Ralph J; Bachoo, Robert M; Mischel, Paul S; Yasui, Wataru; Suematsu, Makoto; Saya, Hideyuki; Soga, Tomoyoshi; Grummt, Ingrid; Bierhoff, Holger; Sasaki, Atsuo T 2019. IMP dehydrogenase-2 drives aberrant nucleolar activity and promotes tumorigenesis in glioblastoma. Nature cell biology, 21 8, 1003-1014

Miller, Taylor E; Henkels, Karen M; Huddleston, Mary; Salisbury, Richard; Hussain, Saber M; Sasaki, Atsuo T; Cho, Kwang-Jin 2019. Depletion of phosphatidylinositol 4-phosphate at the Golgi translocates K-Ras to mitochondria. Journal of cell science, ,

Naffouje, Rand; Grover, Punita; Yu, Hongyang; Sendilnathan, Arun; Wolfe, Kara; Majd, Nazanin; Smith, Eric P; Takeuchi, Koh; Senda, Toshiya; Kofuji, Satoshi; Sasaki, Atsuo T 2019. Anti-Tumor Potential of IMP Dehydrogenase Inhibitors: A Century-Long Story. Cancers, 11 9,

Wolfe, Kara; Kofuji, Satoshi; Yoshino, Hirofumi; Sasaki, Mika; Okumura, Koichi; Sasaki, Atsuo T 2019. Dynamic compartmentalization of purine nucleotide metabolic enzymes at leading edge in highly motile renal cell carcinoma. Biochemical and biophysical research communications, ,

Yoshino, Hirofumi; Yin, Guowei; Kawaguchi, Risa; Popov, Konstantin I; Temple, Brenda; Sasaki, Mika; Kofuji, Satoshi; Wolfe, Kara; Kofuji, Kaori; Okumura, Koichi; Randhawa, Jaskirat; Malhotra, Akshiv; Majd, Nazanin; Ikeda, Yoshiki; Shimada, Hiroko; Kahoud, Emily Rose; Haviv, Sasson; Iwase, Shigeki; Asara, John M; Campbell, Sharon L; Sasaki, Atsuo T 2019. Identification of lysine methylation in the core GTPase domain by GoMADScan. PloS one, 14 8, e0219436

Su, Rui; Dong, Lei; Li, Chenying; Nachtergaele, Sigrid; Wunderlich, Mark; Qing, Ying; Deng, Xiaolan; Wang, Yungui; Weng, Xiaocheng; Hu, Chao; Yu, Mengxia; Skibbe, Jennifer; Dai, Qing; Zou, Dongling; Wu, Tong; Yu, Kangkang; Weng, Hengyou; Huang, Huilin; Ferchen, Kyle; Qin, Xi; Zhang, Bin; Qi, Jun; Sasaki, Atsuo T; Plas, David R; Bradner, James E; Wei, Minjie; Marcucci, Guido; Jiang, Xi; Mulloy, James C; Jin, Jie; He, Chuan; Chen, Jianjun 2018. R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling. Cell, 172 1-2, 90-105.e23

Liu, Hongqi; Feng, Xizhi; Ennis, Kelli N; Behrmann, Catherine A; Sarma, Pranjal; Jiang, Tony T; Kofuji, Satoshi; Niu, Liang; Stratton, Yiwen; Thomas, Hala Elnakat; Yoon, Sang-Oh; Sasaki, Atsuo T; Plas, David R 2017. Pharmacologic Targeting of S6K1 in PTEN-Deficient Neoplasia. Cell reports, 18 9, 2088-2095

Davis, Mindy I; Sasaki, Atsuo T; Simeonov, Anton 2016. Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase alpha in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format. Methods in molecular biology (Clifton, N.J.), 1376 , 1-9

Sasaki, Atsuo T 2016. Dynamic Role of the GTP Energy Metabolism in Cancers. The Keio journal of medicine, 65 1, 21

Sumita, Kazutaka; Lo, Yu-Hua; Takeuchi, Koh; Senda, Miki; Kofuji, Satoshi; Ikeda, Yoshiki; Terakawa, Jumpei; Sasaki, Mika; Yoshino, Hirofumi; Majd, Nazanin; Zheng, Yuxiang; Kahoud, Emily Rose; Yokota, Takehiro; Emerling, Brooke M; Asara, John M; Ishida, Tetsuo; Locasale, Jason W; Daikoku, Takiko; Anastasiou, Dimitrios; Senda, Toshiya; Sasaki, Atsuo T 2016. The Lipid Kinase PI5P4Kbeta Is an Intracellular GTP Sensor for Metabolism and Tumorigenesis Molecular cell, 61 2, 187-98

Takeuchi, Koh; Senda, Miki; Lo, Yu-Hua; Kofuji, Satoshi; Ikeda, Yoshiki; Sasaki, Atsuo T; Senda, Toshiya 2016. Structural reverse genetics study of the PI5P4Kβ-nucleotide complexes reveals the presence of the GTP bioenergetic system in mammalian cells. The FEBS journal, ,

Sumita, Kazutaka; Yoshino, Hirofumi; Sasaki, Mika; Majd, Nazanin; Kahoud, Emily Rose; Takahashi, Hidenori; Takeuchi, Koh; Kuroda, Taruho; Lee, Susan; Charest, Pascale G; Takeda, Kosuke; Asara, John M; Firtel, Richard A; Anastasiou, Dimitrios; Sasaki, Atsuo T 2014. Degradation of activated K-Ras orthologue via K-Ras-specific lysine residues is required for cytokinesis. The Journal of biological chemistry, 289 7, 3950-9

Subramani, Arasukumar; Alsidawi, Samer; Jagannathan, Sajjeev; Sumita, Kazutaka; Sasaki, Atsuo T; Aronow, Bruce; Warnick, Ronald E; Lawler, Sean; Driscoll, James J 2013. The brain microenvironment negatively regulates miRNA-768-3p to promote K-ras expression and lung cancer metastasis. Scientific reports, 3 , 2392

*Wakioka T, *Sasaki A, Kato R, Shouda T, Matsumoto A, Miyoshi K, Tsuneoka M, Komiya S, Baron R, Yoshimura A. . Spred is a Sprouty-related suppressor of Ras signaling Nature., 2001;412(6847): , 647-51

Aman MJ, Migone TS, Sasaki A, Ascherman DP, Zhu M, Soldaini E, Imada K, Miyajima A, Yoshimura A, Leonard WJ. . CIS associates with the interleukin-2 receptor beta chain and inhibits interleukin-2-dependent signaling. J Biol Chem., 1999;274(42): , 30266-72.

Anastasiou D, Poulogiannis G, Asara JM, Bellinger G, Vander Heiden MG, Sasaki AT, Locasale JW and Cantley LC . Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to cellular antioxidant responses Science 2011, 334 , 1248-83

Baker R, Lewis SM, Sasaki AT, Cantley LC, Kuhlman B, Dohlman HG, Campbell SL . Monoubiquitination Activates Ras by Impeding GTPase Activating Protein Function Nat. Stru. Mol. Biol., in press ,

Carracedo A, Ma L, Teruya-Feldstein J, Rojo F, Salmena L, Alimonti A, Egia A, Sasaki AT, Thomas G, Kozma SC, Papa A, Nardella C, Cantley LC, Baselga J, Pandolfi PP . Inhibition of mTORC1 leads to MAPK pathway activation through a PI3K-dependent feedback loop in human cancer J. Clin Invest. 2008, 118 , 3065-74

Charest PG, Shen Z, Lakoduc A, Sasaki AT, Briggs S, Firtel RA . A novel Ras signaling complex controls the RasC-TORC2 pathway and directed cell migration. Dev. Cell 2010, 18 737, 479

Endo T, Sasaki A, Minoguchi M. Joo A, Yoshimura A. . CIS1 interacts with the Y532 of the prolactin receptor and suppresses prolactin-dependent STAT5 activation. J Biochem (Tokyo)., 2003; 133(1): , 109-13

Fujimura K, Sasaki AT, Anderson P. . Selenite targets elF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules Nucleic Acids Res. 2012, Jun , 20

Garcia-Cao I, Song MS, Hobbs RM, Laurent G, Giorgi C. de Boer VC, Anastasiou D, Ito K, Sasaki AT, Rameh L, Carracedo A, Vander Heiden MG, Cantley LC, Pinton P, Haigis MC, Pandolfi PP . Systemic Elevation of PTEN Induces a Tumor-Suppressive Metabolic State Cell 2012, 149 , 49-62

Hanai J, Doro N, Sasaki AT, Kobayashi S, Cantley LC, Seth P, Sukhatme VP . Inhibition of lung cancer growth: ATP citrate lyase knockdown and statin treatment leads to dual blockade of mitogen-actiated protein kinase (MAPK) and phosphatidylinositol-3- kinase (PI3K)/AKT pathways J. Cell Physiol., Jun 17. doi: 10, 1002/jcp.22895.

Inagaki-Ohara K, Chinen T, Matsuzaki G, Sasaki A, Sakamoto Y, Hiromatsu K, Nakamura-Uchiyama F, Nawa Y, Yoshimura A. . Mucosal T cells bearing TCRgammadelta play a protective role in intestinal inflammation. J Immunol., 2004;173(2): , 1390-8

Inagaki-Ohara K, Sasaki A, Matsuzaki G, Ikeda T, Hotokezaka M, Chijiiwa K, Kubo M, Yoshida H, Nawa Y, Yoshimura A. . Suppressor of cytokine signaling 1 in lymphocytes regulates the development of intestinal inflammation in mice. Gut 2006, 55(2) , 212-9

Kuriyama M, Taniguchi T, Shirai Y, Sasaki A, Yoshimura A, Saito N. . Activation and translocation of PKCdelta is necessry for VEGF-induced ERK activation through KDR in HEK293T cells. Biochem Biophys Res Commun. 2004, 325(3) , 843-51

Lee S, Comer FI, Sasaki A, McLeod IX, Duong Y, Okumura K, Yates JR, Parent CA, Firtel RA . TOR complex 2 integrates cell movement during chemotaxis and signal relay in Dictyostelium. Mol Biol Cell 2005, 16(10) , 4572-83

Lehmann U, Schmitz , Weissenbach M, Sobota RM, Hortner M, Friederichs K, Behrmann I, Tsiaris W. Sasaki A, Schneider-Mergener J, Yoshimura A, Neel BG, Heinrich PC, Schaper F. . SHP2 and SOCS3 contribute to Tyr-759-dependent attenuation of interleukin-6 signaling through gp 130. J Biol Chem, 2003;278(1): , 661-71

Locasale JW, Grassian AR, Bass AJ, Heffron G, Metallo CM, Sharfi H, Sasaki AT, Anastasiou D, Vokes NI, Sasaki M, Ligon AH, Beroukhim R, Stephanopoulos G, Asara JM, Myerson M, Wagner G, Brugge JS, Cantley LC and Vander Heiden MG . Amplification of phosphoglycerate dehydrogenase activity in cancer cells diverts glycolytic flux and contributes to oncogenesis Nature Genetics 2011, 43 , 869-74

Marine JC, McKay C, Wang D, Topham DJ, Parganas E, Nakajima H. Pendeville H, Yasukawa H, Sasaki A, Yoshimura A, Ihle JN. . SOCS3 is essential in the regulation of fetal liver erythropoiesis Cell., 1999;98(5): , 617-27.

Masuhara M, Sakamoto H, Matsumoto A, Suzuki R, Yasukawa H, Mitsui K, Wakioka T, Tanimura S, Sasaki A, MisawaH, Yokouchi M, Ohtsubo M, Yoshimura A. . Cloning and characterization of novel CIS family genes Biochem Biophys Res Commun., 1997;239(2): , 4_39-46

Matsumoto, Toshifumi; Ishio, Tetsuya; Sasaki, Atsusi; Aramaki, Masanori; Bandoh, Toshio; Kawano, Katsunori; Yoshida, Takanori; Kitano, Seigo . Pancreatic resection with ultrasonically activated scalpel: preliminary observations. Hepato-gastroenterology, 49 45, 635-8

Nonami A, Kato R, Taniguchi K, Yashiga D, Taketomi T, Fukuyama S, Harada M, Sasaki A, Yoshimura A. . Spred-1 negatively regulates interleukin-3-mediated ERK/mitogen-activated protein (MAP) kinase activation in hematopoietic cells. J Biol Chem, 2004;279(50): , 52543-51

Sakamoto H, Yasukawa H, Masuhara M, Tanimura S, Sasaki A, Yuge K, Ohtsubo M, Ohtsuka A, Fujita T, Ohta T, Furukawa Y, Iwase S, Yamada H, Yoshimura A. . A Janus kinase inhibitor, JAB, is an interferon-gamma-inducible gene and confers resistance to interferons Blood., 1998;92(5): , 1668-76.

Sasaki A, Inagaki-Ohara K, Yoshida T, Yamanaka A, Sasaki M, Yasukawa H, Koromilas AE, Yoshimura A. . The N-terminal tuncated isoform of SOCS3 translated from an alternative initiation AUG condon under stress conditions is stable due to the lack of a major ubiquitination site, Lys-6. J Biol Chem, 2003;278(4): , 2432-6.

Sasaki A, Taketomi T, Kato R, Saeki K, Nonami A, Sasaki M, Kuriyama M, aito N, Shibuya M, Yoshimure A. . Mammalian Sprouty4 suppresses Ras-independent ERK activation by binding to Raf1. Nat Cell Biol., 2003;5(5): , 427-32.

Sasaki A, Taketomi T, Wakioka T, Kato R, Yoshimura A. . Identification of a dominant negative mutant of Sprouty that potentiates fibroblast growth factor- but not epidermal growth factor-induced ERK activation. J Biol Chem. , 2001;26(39): , 36804-8.

Sasaki A, Yasukawa H, Shouda T, Kitamura T, Dikic I, Yoshimura A. . CIS3/SOCS-3 suppresses erythropoietin (EPO) signaling the EPO receptor and JAK2. J Biol Chem., 2000;275(38): , 29338-47.

Sasaki A, Yasukawa H, Suzuki A, Kamizono S, Syoda T, Kinjyo I, Sasaki M, Johnston JA, Yoshimura A. . Cytokine-inducible SH2 protein-3 (CIS3/SOCS3) inhibits Janus tyrosine kinase by binding through the N-terminal kinase inhibitory region as well as SH2 domain Genes Cells., 1999;4(6): , 339-51.

Sasaki AT, Carracedo A, Locasale JW, Anastasiou D, Takeuchi K, Kahoud ER, Haviv S, Asara JM, Pandolfi PP, Cantley LC . Ubiquitination of Ras enhances activaiton and facilitates binding to PI 3-Kinase and Raf Science Signaling. 2011, 4 , 1-9

Sasaki AT, Chun C, Takeda K, Firtel RA, . Localized Ras signaling at the leading edge regulates Pl3K, cell polarity, and directional cell movement. J Cell Biol., 2004; 167(3): , 505-18

Sasaki AT, Janetopoulos C, Lee S, Charest PG, Takeda K, Sundheimer LW, Meili R, Devreotes PN, Firtel RA. . G protein-independent Ras/PI3K/F-actin circuit regulates basic cell motility. J. Cell Biol. 2007, 178 , 185-191

Sporri B, Kovanen PE, Sasaki A, Yoshimura A, Leonard WJ. . JAB/SOCS1/SSI-1 is an interleukin-2-induced inhibitor of IL-2 signaling. Blood., 2001;97(1): , 221-6.

Suzuki R, Sakamoto H, Yasukawa H, Masuhara M, Wakioka T, Sasaki A, Yuge K, Komiya S, Inoue A, Yashimura A. . CIS3 and JAB have different regulatory roles in interleukin-6 mediated differentiation and STAT3 activation n M1 leukemia cells Oncogene., 1988;17(17): , 2271-8.

Takeda K, Sasaki AT, Ha H, Seung HA, Firtel RA. . Role of PI3 kinases in chemotaxis in dictyostelium. J. Biol. Chem. 2007, 283 , 11874-84

Taketomi T, Yoshiga D, Taniguchi K, Kobayashi T, Nonami A, Kato R, Sasaki M, Sasaki A, Ishibashi H, Moriyama M, Nakamura K, Nishimura J, Yoshimura A. . Loss of mammalian Sproty2 leads to enteric neuronal hyperplasia and esophageal achalasia. Nat Neurosci. 205, 8(7) , 855-7

Thou P, Zheng B, Hsu CH, Sasaki AT, Cantley LC . A fluorescent reporter of AMPK activity and cellular energy stress. Cell Metabolism 2011, 13 4, 476-486

Wakioka T, Sasaki A, Mitsui K, Yokouchi M, Inoue A, Komiya S, Yoshimura A. . AAPS, an adaptor protein containing Pleckstrin homology (PH) and Src homology-2 (SH2) domains inhibits the JAK-STAT pathway in collaboration with c-Cbl Leukemia., 1999;13(5): , 760-7.

Wang XS, Shanker S, Dhanasekaran SM, Ateeq B, Sasaki AT, Robinson D, Cao Q, Prensner JR, Yocum AK, Pflueger D, Wang R, Jing X, Fries DF, Asangani IA, Li Y, Cao X, Kumar-Sinha C, Kahoud ER, Omenn GS, Rubin MA, Gopalan A, Reuter V, Palanisamy N, Varambally S, Cantley LC, and Chinnaiyan AM . Amplification Breakpoint Assembly Identifies an Oncogenic KRAS Fusion in Prostate Cancer Cells. Cancer Discovery, 2011 1, 33-41

Yasukawa H, Misawa H, Sakamoto H, Masuhara M, Sasaki A, Wakioka T, Ohtsuka S, Imaizumi T, Matsuda T, Ihle JN, Yoshimura A. . The JAK-binding protein JAB inhibits Janus tyrosine kinase activity through binding in the activatgion loop EMBO J., 1999;18(5): , 1309-20.

Yokouchi M, Wakioka T, Sakamoto H, Yasukawa H, Ohtsuka S, Sasaki A, Ohtsubo M, Valius M, Inoue A, Komiya S, Yoshimura A. . APS, an adaptor protein containing PH and SH2 domains, is associted with the PDGF receptor and c-Cbl and inhibits PDGF-induced mitogenesis Oncogene., 1999;18(3): , 759-67.