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Photo of  Steven J. Kleene, PhD

Steven J. Kleene, PhD

Professor

Pharmacology & Systems Physiology | College of Medicine

Education/Credentials
  • Bachelor's Degree: University of Michigan
  • Doctoral Degree: University of Wisconsin
  • Postdoctoral Fellowship : Northwestern University
Contact Information
Research Interests

The Kleene lab studies signaling in primary cilia as it relates to kidney disease.  Primary cilia are present on most mammalian cells.  They influence fundamental cellular processes, and defects in primary cilia contribute to kidney disease, cancer, and obesity.  Our work focuses on polycystic kidney disease (PKD), a renal disease that is often caused by defects in ion-conducting channels in the primary cilia.  To that end, we have developed...

Peer Reviewed Publications

Kleene, Steven J; Kleene, Nancy K 2021. Inward calcium current through the polycystin-2-dependent channels of renal primary cilia American Journal of Physiology Renal Physiology, 320 , F1165

Kleene, Steven J; Siroky, Brian J; Landero-Figueroa, Julio A; Dixon, Brian P; Pachciarz, Nolan W; Lu, Lu; and Kleene, Nancy K. 2019. The TRPP2-dependent channel of renal primary cilia also requires TRPM3 PLoS ONE, 14 , e0214053.

Kleene, Steven J; Kleene, Nancy K 2017. The native TRPP2-dependent channel of murine renal primary cilia American Journal of Physiology Renal Physiology, 309 , F96

Siroky, Brian J; Kleene, Nancy K; Kleene, Steven J; Varnell, Charles D; Comer, Raven G; Liu, Jialiu; Lu, Lu; Pachciarz, Nolan W; Bissler, John J; Dixon, Bradley P 2017. Primary cilia regulate the osmotic stress response of renal epithelial cells through TRPM3 American Journal of Physiology Renal Physiology, 312 , F791

Nauli, Surya M; Pala, Rajasekharreddy; Kleene, Steven J 2016. Calcium channels in primary cilia Current Opinion in Nephrology and Hypertension, 25 , 452

Flannery, Richard J; Kleene, Nancy K; Kleene, Steven J 2015. A TRPM4-dependent current in murine renal primary cilia American Journal of Physiology Renal Physiology, 309 , F697

Kleene, Steven J; Van Houten, Judith L 2014. Electrical Signaling in Motile and Primary Cilia Bioscience, 64 , 1092-1102

Griff, Edwin R; Kleene, Nancy K; Kleene, Steven J 2012. A selective PMCA inhibitor does not prolong the electroolfactogram in mouse PLoS ONE, 7 , e37148

Kleene, Nancy K; Kleene, Steven J 2012. A method for measuring electrical signals in a primary cilium Cilia, 1 , 17

French, Donald A; Badamdorj, Dorjsuren; Kleene, Steven J 2010. Spatial distribution of calcium-gated chloride channels in olfactory cilia PLoS ONE, 5 , e15676

Kleene, Steven J 2009. Identifying olfaction's 'other channels' The Journal of Physiology, 587 , 4135-4136

Kleene, Steven J 2009. Limits of calcium clearance by plasma membrane calcium ATPase in olfactory cilia PLoS ONE, 4 , e5266

Badamdorj, Dorjsuren; Edwards, David A; French, Donald A; Kleene, Steven J 2008. Identification of Cl(Ca) channel distributions in olfactory cilia Mathematical Methods in the Applied Sciences, 31 , 1860-1873

Kleene, Steven J 2008. The electrochemical basis of odor transduction in vertebrate olfactory cilia Chemical Senses, 33 , 839-859

Smith, David W; Thach, Sokunthirith; Marshall, Erika L; Mendoza, Mary-Grace; Kleene, Steven J 2008. Mice lacking NKCC1 have normal olfactory sensitivity Physiology & Behavior, 93 , 44-49

Nickell, William T; Kleene, Nancy K; Kleene, Steven J 2007. Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium The Journal of Physiology, 583 , 1005-1020

Flannery, Richard J; French, Donald A; Kleene, Steven J 2006. Clustering of cyclic-nucleotide-gated channels in olfactory cilia Biophysical Journal, 91 , 179-188

French, Donald A; Flannery, Richard J; Groetsch, Charles W; Krantz, Willam B; Kleene, Steven J 2006. Numerical approximation of solutions of a nonlinear inverse problem arising in olfaction experimentation Mathematical and Computer Modelling, 43 7-8, 945-956

Nickell, William T; Kleene, Nancy K; Gesteland, Robert C; Kleene, Steven J 2006. Neuronal chloride accumulation in olfactory epithelium of mice lacking NKCC1 Journal of Neurophysiology, 95 , 2003-2006

Pun, Raymund Y K; Kleene, Steven J 2004. An estimate of the resting membrane resistance of frog olfactory receptor neurones The Journal of Physiology, 559 , 535-542

Pun, Raymund Y K; Kleene, Steven J 2003. Contribution of cyclic-nucleotide-gated channels to the resting conductance of olfactory receptor neurons Biophysical Journal, 84 , 3425-3435

Nickell, W T; Pun, R Y K; Bargmann, C I; Kleene, S J 2002. Single ionic channels of two Caenorhabditis elegans chemosensory neurons in native membrane The Journal of Membrane Biology, 189 , 55-66

Pun, Raymund Y K; Kleene, Steven J 2002. Outward currents in olfactory receptor neurons activated by odorants and by elevation of cyclic AMP Cell Biochemistry and Biophysics, 37 , 15-26

Frings, S; Reuter, D; Kleene, S J 2000. Neuronal Ca2+ -activated Cl- channels--homing in on an elusive channel species Progress in Neurobiology, 60 , 247-289

Kleene, S J 2000. Spontaneous gating of olfactory cyclic-nucleotide-gated channels The Journal of Membrane Biology, 178 , 49-54

Kleene, S J 1999. Both external and internal calcium reduce the sensitivity of the olfactory cyclic-nucleotide-gated channel to cAMP Journal of Neurophysiology, 81 , 2675-2682

Kleene, S J 1997. High-gain, low-noise amplification in olfactory transduction Biophysical Journal, 73 , 1110-1117

Larsson, H P; Kleene, S J; Lecar, H 1997. Noise analysis of ion channels in non-space-clamped cables: estimates of channel parameters in olfactory cilia Biophysical Journal, 72 , 1193-1203

Kleene, S J; Pun, R Y 1996. Persistence of the olfactory receptor current in a wide variety of extracellular environments Journal of Neurophysiology, 75 , 1386-1391

Kleene, S J 1995. Block by external calcium and magnesium of the cyclic-nucleotide-activated current in olfactory cilia Neuroscience, 66 , 1001-1008

Kleene, S J 1994. Inhibition of olfactory cyclic nucleotide-activated current by calmodulin antagonists British Journal of Pharmacology, 111 , 469-472

Kleene, S J; Cejtin, H C 1994. Solving buffering problems with Mathematica software Analytical Biochemistry, 222 , 310-314

Kleene, S J; Gesteland, R C; Bryant, S H 1994. An electrophysiological survey of frog olfactory cilia The Journal of Experimental Biology, 195 , 307-328

Pun, R Y; Kleene, S J; Gesteland, R C 1994. Guanine nucleotides modulate steady-state inactivation of voltage-gated sodium channels in frog olfactory receptor neurons The Journal of Membrane Biology, 142 , 103-111

Kleene, S J 1993. Origin of the chloride current in olfactory transduction Neuron, 11 , 123-132

Kleene, S J 1993. The cyclic nucleotide-activated conductance in olfactory cilia: effects of cytoplasmic Mg2+ and Ca2+ The Journal of Membrane Biology, 131 , 237-243

Kleene, S J 1993. A simple intrapipette salt bridge Journal of Neuroscience Methods, 46 , 11-16

Kleene, S J 1992. Basal conductance of frog olfactory cilia Pflügers Archiv : European Journal of Physiology, 421 , 374-380

Kleene, S J; Gesteland, R C 1991. Calcium-activated chloride conductance in frog olfactory cilia The Journal of Neuroscience, 11 , 3624-3629

Kleene, S J; Gesteland, R C 1991. Transmembrane currents in frog olfactory cilia The Journal of Membrane Biology, 120 , 75-81

Lidow, M S; Gesteland, R C; Shipley, M T; Kleene, S J 1987. Comparative study of immature and mature olfactory receptor cells in adult frogs Developmental Brain Research, 31 , 243-258

Kleene, S J 1986. Bacterial chemotaxis and vertebrate olfaction Experientia, 42 , 241-250

Lidow, M S; Kleene, S J; Gesteland, R C 1986. Suppression of mitotic activity and synchronization of cell development in olfactory epithelium Developmental Brain Research, 28 , 145-162

Kleene, S J; Gesteland, R C 1983. Dissociation of frog olfactory epithelium Journal of Neuroscience Methods, 9 , 173-183

Kleene, S J; Gesteland, R C 1981. Dissociation of frog olfactory epithelium with N-ethylmaleimide Brain Research, 229 , 536-540

Kleene, S J; Hobson, A C; Adler, J 1979. Attractants and repellents influence methylation and demethylation of methyl-accepting chemotaxis proteins in an extract of Escherichia coli Proceedings of the National Academy of Sciences of the United States of America, 76 , 6309-6313

Kleene, S J; Toews, M L; Adler, J 1977. Isolation of glutamic acid methyl ester from an Escherichia coli membrane protein involved in chemotaxis The Journal of Biological Chemistry, 252 , 3214-3218