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Photo of  Brian Gebelein, PhD

Brian Gebelein, PhD


Pediatrics | College of Medicine

  • Ph.D.: Mayo Graduate School
  • B.S.: University of Wisconsin
Contact Information
Research Interests

The human body is composed of an estimated 50 trillion cells that can be classified into
approximately 210 different cell types. Altogether, these cells form organ systems that perform specialized functions required for digestion, respiration, immunity, locomotion, sensory detection, and decision-making. Amazingly, each cell type arises from a common fertilized egg, and with very rare exceptions, contains an identical set of genomic instructions. Unfortunately, our lack of understanding of how the ...

Peer Reviewed Publications

Del Águila, Ángela; Adam, Mike; Ullom, Kristy; Shaw, Nicholas; Qin, Shenyue; Ehrman, Jacqueline; Nardini, Diana; Salomone, Joseph; Gebelein, Brian; Lu, Q Richard; Potter, Steven S; Waclaw, Ronald; Campbell, Kenneth; Nakafuku, Masato 2022. Olig2 defines a subset of neural stem cells that produce specific olfactory bulb interneuron subtypes in the subventricular zone of adult mice. Development (Cambridge, England), 149 5,

Neilson, Derek E; Zech, Michael; Hufnagel, Robert B; Slone, Jesse; Wang, Xinjian; Homan, Shelli; Gutzwiller, Lisa M; Leslie, Elizabeth J; Leslie, Nancy D; Xiao, Jianfeng; Hedera, Peter; LeDoux, Mark S; Gebelein, Brian; Wilbert, Friederike; Eckenweiler, Matthias; Winkelmann, Juliane; Gilbert, Donald L; Huang, Taosheng 2022. A Novel Variant of ATP5MC3 Associated with Both Dystonia and Spastic Paraplegia. Movement disorders : official journal of the Movement Disorder Society, 37 2, 375-383

Cain, Brittany; Gebelein, Brian 2021. Mechanisms Underlying Hox-Mediated Transcriptional Outcomes. Frontiers in cell and developmental biology, 9 , 787339

Kuang, Yi; Pyo, Anna; Eafergan, Natanel; Cain, Brittany; Gutzwiller, Lisa M; Axelrod, Ofri; Gagliani, Ellen K; Weirauch, Matthew T; Kopan, Raphael; Kovall, Rhett A; Sprinzak, David; Gebelein, Brian 2021. Enhancers with cooperative Notch binding sites are more resistant to regulation by the Hairless co-repressor. PLoS genetics, 17 9, e1009039

Oatman, Nicole; Dasgupta, Nupur; Arora, Priyanka; Choi, Kwangmin; Gawali, Mruniya V; Gupta, Nishtha; Parameswaran, Sreeja; Salomone, Joseph; Reisz, Julie A; Lawler, Sean; Furnari, Frank; Brennan, Cameron; Wu, Jianqiang; Sallans, Larry; Gudelsky, Gary; Desai, Pankaj; Gebelein, Brian; Weirauch, Matthew T; D'Alessandro, Angelo; Komurov, Kakajan; Dasgupta, Biplab 2021. Mechanisms of stearoyl CoA desaturase inhibitor sensitivity and acquired resistance in cancer. Science advances, 7 7,

Salomone, Joseph; Qin, Shenyue; Fufa, Temesgen D; Cain, Brittany; Farrow, Edward; Guan, Bin; Hufnagel, Robert B; Nakafuku, Masato; Lim, Hee-Woong; Campbell, Kenneth; Gebelein, Brian 2021. Conserved Gsx2/Ind homeodomain monomer versus homodimer DNA binding defines regulatory outcomes in flies and mice. Genes & development, 35 1-2, 157-174

Elliott, Kelsey H; Chen, Xiaoting; Salomone, Joseph; Chaturvedi, Praneet; Schultz, Preston A; Balchand, Sai K; Servetas, Jeffrey D; Zuniga, Aimée; Zeller, Rolf; Gebelein, Brian; Weirauch, Matthew T; Peterson, Kevin A; Brugmann, Samantha A 2020. Gli3 utilizes Hand2 to synergistically regulate tissue-specific transcriptional networks. eLife, 9 ,

Kobia, Francis M; Preusse, Kristina; Dai, Quanhui; Weaver, Nicholas; Hass, Matthew R; Chaturvedi, Praneet; Stein, Sarah J; Pear, Warren S; Yuan, Zhenyu; Kovall, Rhett A; Kuang, Yi; Eafergen, Natanel; Sprinzak, David; Gebelein, Brian; Brunskill, Eric W; Kopan, Raphael 2020. Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation. PLoS biology, 18 10, e3000850

Kuang, Yi; Golan, Ohad; Preusse, Kristina; Cain, Brittany; Christensen, Collin J; Salomone, Joseph; Campbell, Ian; Okwubido-Williams, FearGod V; Hass, Matthew R; Yuan, Zhenyu; Eafergan, Nathanel; Moberg, Kenneth H; Kovall, Rhett A; Kopan, Raphael; Sprinzak, David; Gebelein, Brian 2020. Enhancer architecture sensitizes cell specific responses to Notch gene dose via a bind and discard mechanism. eLife, 9 ,

Roychoudhury, Kaushik; Salomone, Joseph; Qin, Shenyue; Cain, Brittany; Adam, Mike; Potter, S Steven; Nakafuku, Masato; Gebelein, Brian; Campbell, Kenneth 2020. Physical interactions between Gsx2 and Ascl1 balance progenitor expansion versus neurogenesis in the mouse lateral ganglionic eminence. Development (Cambridge, England), 147 7,

Zandvakili, Arya; Uhl, Juli D; Campbell, Ian; Salomone, Joseph; Song, Yuntao Charlie; Gebelein, Brian 2019. The cis-regulatory logic underlying abdominal Hox-mediated repression versus activation of regulatory elements in Drosophila. Developmental biology, 445 2, 226-236

Zhang, Xinghao; McGrath, Patrick S; Salomone, Joseph; Rahal, Mohamed; McCauley, Heather A; Schweitzer, Jamie; Kovall, Rhett; Gebelein, Brian; Wells, James M 2019. A Comprehensive Structure-Function Study of Neurogenin3 Disease-Causing Alleles during Human Pancreas and Intestinal Organoid Development. Developmental cell, 50 3, 367-380.e7

Zandvakili, Arya; Campbell, Ian; Gutzwiller, Lisa M; Weirauch, Matthew T; Gebelein, Brian 2018. Degenerate Pax2 and Senseless binding motifs improve detection of low-affinity sites required for enhancer specificity. PLoS genetics, 14 4, e1007289

Gu, Zirong; Kalambogias, John; Yoshioka, Shin; Han, Wenqi; Li, Zhuo; Kawasawa, Yuka Imamura; Pochareddy, Sirisha; Li, Zhen; Liu, Fuchen; Xu, Xuming; Wijeratne, H. R. Sagara; Ueno, Masaki; Blatz, Emily; Salomone, Joseph; Kumanogoh, Atsushi; Rasin, Mladen-Roko; Gebelein, Brian; Weirauch, Matthew T; Sestan, Nenad; Martin, John H; Yoshida, Yutaka 2017. Control of species-dependent cortico-motoneuronal connections underlying manual dexterity. Science (New York, N.Y.), 357 6349, 400-404

Kovall, Rhett A; Gebelein, Brian; Sprinzak, David; Kopan, Raphael 2017. The Canonical Notch Signaling Pathway: Structural and Biochemical Insights into Shape, Sugar, and Force. Developmental cell, 41 3, 228-241

Wang, Guolun; Gutzwiller, Lisa; Li-Kroeger, David; Gebelein, Brian 2017. A Hox complex activates and potentiates the Epidermal Growth Factor signaling pathway to specify Drosophila oenocytes. PLoS genetics, 13 7, e1006910

Uhl, Juli D; Zandvakili, Arya; Gebelein, Brian 2016. A Hox Transcription Factor Collective Binds a Highly Conserved Distal-less cis-Regulatory Module to Generate Robust Transcriptional Outcomes. PLoS genetics, 12 4, e1005981

Zandvakili, Arya; Gebelein, Brian 2016. Mechanisms of Specificity for Hox Factor Activity. Journal of developmental biology, 4 2,

Gresser, Amy L; Gutzwiller, Lisa M; Gauck, Mackenzie K; Hartenstein, Volker; Cook, Tiffany A; Gebelein, Brian 2015. Rhomboid Enhancer Activity Defines a Subset of Drosophila Neural Precursors Required for Proper Feeding, Growth and Viability. PloS one, 10 8, e0134915

Velu, Chinavenmeni S; Chaubey, Aditya; Phelan, James D; Horman, Shane R; Wunderlich, Mark; Guzman, Monica L; Jegga, Anil G; Zeleznik-Le, Nancy J; Chen, Jianjun; Mulloy, James C; Cancelas, Jose A; Jordan, Craig T; Aronow, Bruce J; Marcucci, Guido; Bhat, Balkrishen; Gebelein, Brian; Grimes, H Leighton 2014. Therapeutic antagonists of microRNAs deplete leukemia-initiating cell activity. The Journal of clinical investigation, 124 1, 222-36

Jukam, David; Xie, Baotong; Rister, Jens; Terrell, David; Charlton-Perkins, Mark; Pistillo, Daniela; Gebelein, Brian; Desplan, Claude; Cook, Tiffany 2013. Opposite feedbacks in the Hippo pathway for growth control and neural fate. Science (New York, N.Y.), 342 6155, 1238016

Bryantsev, Anton L; Duong, Sandy; Brunetti, Tonya M; Chechenova, Maria B; Lovato, TyAnna L; Nelson, Cloyce; Shaw, Elizabeth; Uhl, Juli D; Gebelein, Brian; Cripps, Richard M 2012. Extradenticle and homothorax control adult muscle fiber identity in Drosophila. Developmental cell, 23 3, 664-73

Burns, Kevin A; Gutzwiller, Lisa M; Tomoyasu, Yoshinori; Gebelein, Brian 2012. Oenocyte development in the red flour beetle Tribolium castaneum. Development genes and evolution, 222 2, 77-88

Fujioka, Miki; Gebelein, Brian; Cofer, Zenobia C; Mann, Richard S; Jaynes, James B 2012. Engrailed cooperates directly with Extradenticle and Homothorax on a distinct class of homeodomain binding sites to repress sloppy paired. Developmental biology, 366 2, 382-92

Li-Kroeger, David; Cook, Tiffany A; Gebelein, Brian 2012. Integration of an abdominal Hox complex with Pax2 yields cell-specific EGF secretion from Drosophila sensory precursor cells. Development (Cambridge, England), 139 9, 1611-9

Terrell, David; Xie, Baotong; Workman, Michael; Mahato, Simpla; Zelhof, Andrew; Gebelein, Brian; Cook, Tiffany 2012. OTX2 and CRX rescue overlapping and photoreceptor-specific functions in the Drosophila eye. Developmental dynamics : an official publication of the American Association of Anatomists, 241 1, 215-28

Charlton-Perkins, Mark; Whitaker, S Leigh; Fei, Yueyang; Xie, Baotong; Li-Kroeger, David; Gebelein, Brian; Cook, Tiffany 2011. Prospero and Pax2 combinatorially control neural cell fate decisions by modulating Ras- and Notch-dependent signaling. Neural development, 6 , 20

Johnston, Robert J; Otake, Yoshiaki; Sood, Pranidhi; Vogt, Nina; Behnia, Rudy; Vasiliauskas, Daniel; McDonald, Elizabeth; Xie, Baotong; Koenig, Sebastian; Wolf, Reinhard; Cook, Tiffany; Gebelein, Brian; Kussell, Edo; Nakagoshi, Hideki; Desplan, Claude 2011. Interlocked feedforward loops control cell-type-specific Rhodopsin expression in the Drosophila eye. Cell, 145 6, 956-68

Gutzwiller, Lisa M; Witt, Lorraine M; Gresser, Amy L; Burns, Kevin A; Cook, Tiffany A; Gebelein, Brian 2010. Proneural and abdominal Hox inputs synergize to promote sensory organ formation in the Drosophila abdomen. Developmental biology, 348 2, 231-43

Lomberk, Gwen A; Imoto, Issei; Gebelein, Brian; Urrutia, Raul; Cook, Tiffany A 2010. Conservation of the TGFbeta/Labial homeobox signaling loop in endoderm-derived cells between Drosophila and mammals. Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 10 1, 74-84

McDonald, Elizabeth C; Xie, Baotong; Workman, Michael; Charlton-Perkins, Mark; Terrell, David A; Reischl, Joachim; Wimmer, Ernst A; Gebelein, Brian A; Cook, Tiffany A 2010. Separable transcriptional regulatory domains within Otd control photoreceptor terminal differentiation events. Developmental biology, 347 1, 122-32

Phelan, James D; Shroyer, Noah F; Cook, Tiffany; Gebelein, Brian; Grimes, H Leighton 2010. Gfi1-cells and circuits: unraveling transcriptional networks of development and disease. Current opinion in hematology, 17 4, 300-7

Uhl, Juli D; Cook, Tiffany A; Gebelein, Brian 2010. Comparing anterior and posterior Hox complex formation reveals guidelines for predicting cis-regulatory elements. Developmental biology, 343 1-2, 154-66

Witt, Lorraine M; Gutzwiller, Lisa M; Gresser, Amy L; Li-Kroeger, David; Cook, Tiffany A; Gebelein, Brian 2010. Atonal, Senseless, and Abdominal-A regulate rhomboid enhancer activity in abdominal sensory organ precursors. Developmental biology, 344 2, 1060-70

Horman, Shane R; Velu, Chinavenmeni S; Chaubey, Aditya; Bourdeau, Tristan; Zhu, Jinfang; Paul, William E; Gebelein, Brian; Grimes, H Leighton 2009. Gfi1 integrates progenitor versus granulocytic transcriptional programming. Blood, 113 22, 5466-75

Li-Kroeger, David; Witt, Lorraine M; Grimes, H Leighton; Cook, Tiffany A; Gebelein, Brian 2008. Hox and senseless antagonism functions as a molecular switch to regulate EGF secretion in the Drosophila PNS. Developmental cell, 15 2, 298-308

Gebelein, Brian; Mann, Richard S 2007. Compartmental modulation of abdominal Hox expression by engrailed and sloppy-paired patterns the fly ectoderm. Developmental biology, 308 2, 593-605

Xie, Baotong; Charlton-Perkins, Mark; McDonald, Elizabeth; Gebelein, Brian; Cook, Tiffany 2007. Senseless functions as a molecular switch for color photoreceptor differentiation in Drosophila. Development (Cambridge, England), 134 23, 4243-53

Gebelein, Brian; McKay, Daniel J; Mann, Richard S 2004. Direct integration of Hox and segmentation gene inputs during Drosophila development. Nature, 431 7009, 653-9

Bessa, Jose; Gebelein, Brian; Pichaud, Franck; Casares, Fernando; Mann, Richard S 2002. Combinatorial control of Drosophila eye development by eyeless, homothorax, and teashirt. Genes & development, 16 18, 2415-27

Gebelein, Brian; Culi, Joaquim; Ryoo, Hyung Don; Zhang, Wen; Mann, Richard S 2002. Specificity of Distalless repression and limb primordia development by abdominal Hox proteins. Developmental cell, 3 4, 487-98

Gebelein, Brian . The control of EGF signaling and cell fate in the Drosophila abdomen. Fly, 2 5, 257-8