Flow Cytometry Core
The Department of Molecular Genetics houses a FACSCalibur four color flow cytometer for use by researchers. The instrument is available seven days a week 24 hours per day.
The instrument is available on a first-come, first serve basis and researchers can sign up online to reserve their time on the machine.
For access to the instrument and the online calendar, please contact Dr. Miller.
The FACSCalibur is configured as following:
- FL1 Channel - Blue Laser (488nm) and 515-545nm Emission Filter (FITC, GFP, etc.)
- FL2 Channel - Blue Laser (488nm) and 564-601nm Emission Filter (PE, PI, RFP, etc.)
- FL3 Channel - Blue Laser (488nm) and 670LP Emission Filter (7-AAD, PerCP, etc)
- FL4 Channel - Red Laser (635nm) and 653-669nm Emission Filter (APC, Cy5, etc.)
It is running the BS CellQuest Pro Software.
The flow cytometer is located in CARE 2876.
Our facility does not have the capacity to handle cell sorting. If you need to sort cells or for advice on cell sorting please visit our colleagues at the Shriner's Hospital Flow Core.
Usage rates are $20.00 per 1/2 hour (1/2 hour is the minimum charge). Bills are processed quarterly and sent to the investigator.
If you would like more information regarding use of the flow cytometer, please contact:
William Miller, PhD
Department of Molecular Genetics
Biochemistry & Microbiology
Medical Sciences Building Room 1654
231 Albert Sabin Way
PO Box 670524
Cincinnati, OH 45267-0524