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The Flow Core supports a Beckman CytoFLEX S with 9 detectors and 4 lasers in CARE 2874 and a FACSCalibur 4 color flow cytometer system in CARE 2876. Access is available 24 hrs a day and 7 days per week once investigators are registered in Stratocore and approved by the core director.
To book equipment and/or access services from the Flow Core, please login or create an account in Stratocore at https://ppms.us/uc/start/.
For access to the instruments and the online calendar, please contact Dr. Miller.
The FACSCalibur is configured as follows:
Here are the channels currently configured (in bold) the ones in italics can be utilized by repositioning the filters, which is relatively easy to do.
CytoFLEX (4 laser, 9 color)
Violet Laser (405nm) Emmision Filter
PB450 (PacBlue, eFluor450) 450/45
KO525 (Krome Orange, Zombie Aqua) 525/40
Blue Laser (488nm)
FITC (GFP, Alexa488, Venus) 525/40
PerCP (PC5, PC5.5) 690/50
Yellow/Green Laser (561nm)
PE (PI, Sytox Orange, DsRed, TdTomato) 585/42
ECD (mCherry, Texas Red) 610/20
Red Laser (638nm)
APC (AlexaFluor 647, eFluor660) 660/10
APC-A750 (APC-Cy7, APC eFluor780 780/60
APC-A700 (AlexaFluor 700)
CARE 2874-2876213 Albert Sabin WayCincinnati45267-0524
Medical Sciences Building231 Albert Sabin WayPO Box 670524Cincinnati, OH 45267-0524Phone: 513-558-2366Email: email@example.com