Paul R. Rosevear, PhD

Michalek, A.J., Gulick, J., Robbins, J. Howarth, J.W., Rosevear, P.R. and Warshaw, D.M. 2013. Phosphorylation modulates mechanical stability of the cardiac myosin binding protein-C motif Biophysical Journal-Biophysical Letters, 104 2, 442

Howarth, Jack W; Ramisetti, Srinivas; Nolan, Kristof; Sadayappan, Sakthivel; Rosevear, Paul R 2012. Structural insight into unique cardiac myosin-binding protein-C motif: a partially folded domain. The Journal of biological chemistry, 287 11, 8254-62

Millard, Ronald W; Rosevear, Paul R 2012. Metabolomics: seeking a unique biomarker signature for coronary artery syndromes. Journal of the American College of Cardiology, 59 18, 1642-4

Sadayappan, Sakthivel; Finley, Natosha; Howarth, Jack W; Osinska, Hanna; Klevitsky, Raisa; Lorenz, John N; Rosevear, Paul R; Robbins, Jeffrey 2008. Role of the acidic N' region of cardiac troponin I in regulating myocardial function. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 22 4, 1246-57

Solaro, R John; Rosevear, Paul; Kobayashi, Tomoyoshi 2008. The unique functions of cardiac troponin I in the control of cardiac muscle contraction and relaxation. Biochemical and biophysical research communications, 369 1, 82-7

Howarth, Jack W; Meller, Jarek; Solaro, R John; Trewhella, Jill; Rosevear, Paul R 2007. Phosphorylation-dependent conformational transition of the cardiac specific N-extension of troponin I in cardiac troponin. Journal of molecular biology, 373 3, 706-22

Sakthivel, Sadayappan; Finley, Natosha L; Rosevear, Paul R; Lorenz, John N; Gulick, James; Kim, Scott; VanBuren, Peter; Martin, Lisa A; Robbins, Jeffrey 2005. In vivo and in vitro analysis of cardiac troponin I phosphorylation. The Journal of biological chemistry, 280 1, 703-14

Finley, Natosha L; Howarth, Jack W; Rosevear, Paul R 2004. Structure of the Mg2+-loaded C-lobe of cardiac troponin C bound to the N-domain of cardiac troponin I: comparison with the Ca2+-loaded structure. Biochemistry, 43 36, 11371-9

Finley, Natosha L; Rosevear, Paul R 2004. Introduction of negative charge mimicking protein kinase C phosphorylation of cardiac troponin I. Effects on cardiac troponin C. The Journal of biological chemistry, 279 52, 54833-40

Sorsa, Tia; Pollesello, Piero; Rosevear, Paul R; Drakenberg, Torbjörn; Kilpeläinen, Ilkka 2004. Stereoselective binding of levosimendan to cardiac troponin C causes Ca2+-sensitization. European journal of pharmacology, 486 1, 1-8

Heller, William T; Finley, Natosha L; Dong, Wen-Ji; Timmins, Peter; Cheung, Herbert C; Rosevear, Paul R; Trewhella, Jill 2003. Small-angle neutron scattering with contrast variation reveals spatial relationships between the three subunits in the ternary cardiac troponin complex and the effects of troponin I phosphorylation. Biochemistry, 42 25, 7790-800

Rosevear, Paul R; Finley, Natosha 2003. Molecular mechanism of levosimendan action: an update. Journal of molecular and cellular cardiology, 35 9, 1011-5

Dvoretsky, Alex; Abusamhadneh, Ekram M; Howarth, Jack W; Rosevear, Paul R 2002. Solution structure of calcium-saturated cardiac troponin C bound to cardiac troponin I. The Journal of biological chemistry, 277 41, 38565-70

Dvoretsky, Alex; Gaponenko, Vadim; Rosevear, Paul R 2002. Derivation of structural restraints using a thiol-reactive chelator. FEBS letters, 528 1-3, 189-92

Heller, William T; Abusamhadneh, Ekram; Finley, Natosha; Rosevear, Paul R; Trewhella, Jill 2002. The solution structure of a cardiac troponin C-troponin I-troponin T complex shows a somewhat compact troponin C interacting with an extended troponin I-troponin T component. Biochemistry, 41 52, 15654-63

Abbott, M B; Dong, W J; Dvoretsky, A; DaGue, B; Caprioli, R M; Cheung, H C; Rosevear, P R 2001. Modulation of cardiac troponin C-cardiac troponin I regulatory interactions by the amino-terminus of cardiac troponin I. Biochemistry, 40 20, 5992-6001

Abusamhadneh, E; Abbott, M B; Dvoretsky, A; Finley, N; Sasi, S; Rosevear, P R 2001. Interaction of bepridil with the cardiac troponin C/troponin I complex. FEBS letters, 506 1, 51-4

Sorsa, T; Heikkinen, S; Abbott, M B; Abusamhadneh, E; Laakso, T; Tilgmann, C; Serimaa, R; Annila, A; Rosevear, P R; Drakenberg, T; Pollesello, P; Kilpelainen, I 2001. Binding of levosimendan, a calcium sensitizer, to cardiac troponin C. The Journal of biological chemistry, 276 12, 9337-43

Abbott, M B; Dvoretsky, A; Gaponenko, V; Rosevear, P R 2000. Cardiac troponin I inhibitory peptide: location of interaction sites on troponin C. FEBS letters, 469 2-3, 168-72

Abbott, M B; Gaponenko, V; Abusamhadneh, E; Finley, N; Li, G; Dvoretsky, A; Rance, M; Solaro, R J; Rosevear, P R 2000. Regulatory domain conformational exchange and linker region flexibility in cardiac troponin C bound to cardiac troponin I. The Journal of biological chemistry, 275 27, 20610-7

Finley, N; Dvoretsky, A; Rosevear, P R 2000. Magnesium-calcium exchange in cardiac troponin C bound to cardiac troponin I. Journal of molecular and cellular cardiology, 32 8, 1439-46

Gaponenko, V; Dvoretsky, A; Walsby, C; Hoffman, B M; Rosevear, P R 2000. Calculation of z-coordinates and orientational restraints using a metal binding tag. Biochemistry, 39 49, 15217-24

Gaponenko, V; Howarth, J W; Columbus, L; Gasmi-Seabrook, G; Yuan, J; Hubbell, W L; Rosevear, P R 2000. Protein global fold determination using site-directed spin and isotope labeling. Protein science : a publication of the Protein Society, 9 2, 302-9

Permi, P; Rosevear, P R; Annila, A 2000. A set of HNCO-based experiments for measurement of residual dipolar couplings in 15N, 13C, (2H)-labeled proteins. Journal of biomolecular NMR, 17 1, 43-54

Finley, N; Abbott, M B; Abusamhadneh, E; Gaponenko, V; Dong, W; Gasmi-Seabrook, G; Howarth, J W; Rance, M; Solaro, R J; Cheung, H C; Rosevear, P R 1999. NMR analysis of cardiac troponin C-troponin I complexes: effects of phosphorylation. FEBS letters, 453 1-2, 107-12

Gaponenko, V; Abusamhadneh, E; Abbott, M B; Finley, N; Gasmi-Seabrook, G; Solaro, R J; Rance, M; Rosevear, P R 1999. Effects of troponin I phosphorylation on conformational exchange in the regulatory domain of cardiac troponin C. The Journal of biological chemistry, 274 24, 16681-4

Gasmi-Seabrook, G M; Howarth, J W; Finley, N; Abusamhadneh, E; Gaponenko, V; Brito, R M; Solaro, R J; Rosevear, P R 1999. Solution structures of the C-terminal domain of cardiac troponin C free and bound to the N-terminal domain of cardiac troponin I. Biochemistry, 38 26, 8313-22

Howarth, J W; Krudy, G A; Lin, X; Putkey, J A; Rosevear, P R 1995. An NMR and spin label study of the effects of binding calcium and troponin I inhibitory peptide to cardiac troponin C. Protein science : a publication of the Protein Society, 4 4, 671-80

Kleerekoper, Q; Howarth, J W; Guo, X; Solaro, R J; Rosevear, P R 1995. Cardiac troponin I induced conformational changes in cardiac troponin C as monitored by NMR using site-directed spin and isotope labeling. Biochemistry, 34 41, 13343-52

Zhou, L; Rosevear, P R 1995. Mutation of the carboxy terminal zinc finger of E. coli isoleucyl-tRNA synthetase alters zinc binding and aminoacylation activity. Biochemical and biophysical research communications, 216 2, 648-54

Jones, C M; Reyes, C J; Cook, R G; Krudy, G A; Rosevear, P R 1994. Synthetic macrophage activating peptides derived from the N-terminus of human MCF. Biochemical and biophysical research communications, 199 1, 20-5

Krudy, G A; Kleerekoper, Q; Guo, X; Howarth, J W; Solaro, R J; Rosevear, P R 1994. NMR studies delineating spatial relationships within the cardiac troponin I-troponin C complex. The Journal of biological chemistry, 269 38, 23731-5

Lin, X; Krudy, G A; Howarth, J; Brito, R M; Rosevear, P R; Putkey, J A 1994. Assignment and calcium dependence of methionyl epsilon C and epsilon H resonances in cardiac troponin C. Biochemistry, 33 48, 14434-42

Xu, B; Trawick, B; Krudy, G A; Phillips, R M; Zhou, L; Rosevear, P R 1994. Probing the metal binding sites of Escherichia coli isoleucyl-tRNA synthetase. Biochemistry, 33 2, 398-402

Brito, R M; Krudy, G A; Negele, J C; Putkey, J A; Rosevear, P R 1993. Calcium plays distinctive structural roles in the N- and C-terminal domains of cardiac troponin C. The Journal of biological chemistry, 268 28, 20966-73

Xu, B; Krudy, G A; Rosevear, P R 1993. Identification of the metal ligands and characterization of a putative zinc finger in methionyl-tRNA synthetase. The Journal of biological chemistry, 268 22, 16259-64

Krudy, G A; Brito, R M; Putkey, J A; Rosevear, P R 1992. Conformational changes in the metal-binding sites of cardiac troponin C induced by calcium binding. Biochemistry, 31 6, 1595-602

Brito, R M; Putkey, J A; Strynadka, N C; James, M N; Rosevear, P R 1991. Comparative NMR studies on cardiac troponin C and a mutant incapable of binding calcium at site II. Biochemistry, 30 42, 10236-45

Brito, R M; Rudolph, F B; Rosevear, P R 1991. Conformation of NADP+ bound to a type II dihydrofolate reductase. Biochemistry, 30 6, 1461-9

Williams, J S; Rosevear, P R 1991. A novel alpha-proton exchange reaction catalyzed by Escherichia coli methionyl-tRNA synthetase. Biochemistry, 30 26, 6412-6

Williams, J S; Rosevear, P R 1991. Nuclear overhauser effect studies of the conformations of Mg(alpha, beta-methylene)ATP bound to E. coli isoleucyl-tRNA synthetase. Biochemical and biophysical research communications, 176 2, 682-9

Williams, J S; Rosevear, P R 1991. Nuclear Overhauser effect studies on the conformations of Mg(alpha,beta-methylene)ATP bound to Escherichia coli methionyl-tRNA synthetase. The Journal of biological chemistry, 266 4, 2089-98

Brito, R M; Reddick, R; Bennett, G N; Rudolph, F B; Rosevear, P R 1990. Characterization and stereochemistry of cofactor oxidation by a type II dihydrofolate reductase. Biochemistry, 29 42, 9825-31

Flores-Carreón, A; Hixson, S H; Gómez, A; Shao, M C; Krudy, G; Rosevear, P R; Wold, F 1990. The processing of N-linked glycans in yeast. Mutually exclusive steps in the processing of a Man6 derivative by yeast membrane preparations. The Journal of biological chemistry, 265 2, 754-9

Sweeney, H L; Brito, R M; Rosevear, P R; Putkey, J A 1990. The low-affinity Ca2(+)-binding sites in cardiac/slow skeletal muscle troponin C perform distinct functions: site I alone cannot trigger contraction. Proceedings of the National Academy of Sciences of the United States of America, 87 24, 9538-42

Rosevear, P R; Mildvan, A S 1989. Ligand conformations and ligand-enzyme interactions as studied by the nuclear Overhauser effect. Methods in enzymology, 177 , 333-58

Shao, M C; Krudy, G; Rosevear, P R; Wold, F 1989. Protein matrix effects on glycan processing by mannosidase II and sialyl transferase from rat liver. Biochemistry, 28 9, 4077-83

Rosevear, P R 1988. Purification and NMR studies of [methyl-13C]methionine-labeled truncated methionyl-tRNA synthetase. Biochemistry, 27 20, 7931-9

Rosevear, P R; Fox, T L; Mildvan, A S 1987. Nuclear Overhauser effect studies of the conformations of MgATP bound to the active and secondary sites of muscle pyruvate kinase. Biochemistry, 26 12, 3487-93

Rosevear, P R; Powers, V M; Dowhan, D; Mildvan, A S; Kenyon, G L 1987. Nuclear overhauser effect studies on the conformation of magnesium adenosine 5'-triphosphate bound to rabbit muscle creatine kinase. Biochemistry, 26 17, 5338-44

Mildvan, A S; Rosevear, P R; Fry, D C; Bramson, H N; Kaiser, E T 1985. NMR studies of the mechanism of action and regulation of protein kinase. Current topics in cellular regulation, 27 , 133-44

Mildvan, A S; Kaiser, E T; Rosevear, P R; Bramson, H N 1984. NMR studies of the mechanism of cyclic AMP-dependent protein kinase. Federation proceedings, 43 11, 2634-9

Rosevear, P R; Fry, D C; Mildvan, A S; Doughty, M; O'Brian, C; Kaiser, E T 1984. NMR studies of the backbone protons and secondary structure of pentapeptide and heptapeptide substrates bound to bovine heart protein kinase. Biochemistry, 23 14, 3161-73

Rosevear, P R; Sellin, S; Mannervik, B; Kuntz, I D; Mildvan, A S 1984. NMR and computer modeling studies of the conformations of glutathione derivatives at the active site of glyoxalase I. The Journal of biological chemistry, 259 18, 11436-47

Mildvan, A S; Rosevear, P R; Granot, J; O 1983. Use of NMR and EPR to study cAMP-dependent protein kinase. Methods in enzymology, 99 , 93-119

Roff, C F; Rosevear, P R; Wang, J L; Barker, R 1983. Identification of carbohydrate-binding proteins from mouse and human fibroblasts. The Biochemical journal, 211 3, 625-9

Rosevear, P R; Bramson, H N; O'Brian, C; Kaiser, E T; Mildvan, A S 1983. Nuclear Overhauser effect studies of the conformations of tetraamminecobalt(III)-adenosine 5'-triphosphate free and bound to bovine heart protein kinase. Biochemistry, 22 14, 3439-47

Rosevear, P R; Chari, R V; Kozarich, J W; Sellin, S; Mannervik, B; Mildvan, A S 1983. 13C NMR studies of the product complex of glyoxalase I. The Journal of biological chemistry, 258 11, 6823-6

Barker, R; Nunez, H A; Rosevear, P; Serianni, A S 1982. 13C NMR analysis of complex carbohydrates. Methods in enzymology, 83 , 58-69

Rosevear, P R; Nunez, H A; Barker, R 1982. Synthesis and solution conformation of the type 2 blood group oligosaccharide alpha LFuc(1 leads to 2) beta DGal(1 leads to 4) beta DGlcNAc. Biochemistry, 21 6, 1421-31

Sellin, S; Rosevear, P R; Mannervik, B; Mildvan, A S 1982. Nuclear relaxation studies of the role of the essential metal in glyoxalase I. The Journal of biological chemistry, 257 17, 10023-9

Rosevear, P R; Desmeules, P; Kenyon, G L; Mildvan, A S 1981. Nuclear magnetic resonance studies of the role of histidine residues at the active site of rabbit muscle creatine kinase. Biochemistry, 20 21, 6155-64

Rosevear, P; VanAken, T; Baxter, J; Ferguson-Miller, S 1980. Alkyl glycoside detergents: a simpler synthesis and their effects on kinetic and physical properties of cytochrome c oxidase. Biochemistry, 19 17, 4108-15