Determinations of beta-hydroxybutyrate in plasma/serum/lymph will be made using a specific colorimetric assay. Reactions will be run in microtiter plates and analyzed on a plate reader. Using this method assays can be performed on samples of plasma or serum.

Concentrations of adiponectin in plasma or serum are determined by ELISA.

Concentrations of c-peptide in plasma or serum are determined by ELISA

Concentrations of glucagon in plasma/serum are measured using an ELISA kit from Millipore.

Glucagon-like peptide 1 (GLP-1) concentrations are measured by ELISA.

Glucagon-like peptide 1 (GLP-1) concentrations are measured by ELISA.

Determinations of glucose in plasma/serum/lymph will be made using a specific colorimetric assay. Reactions will be run in microtiter plates and analyzed on a plate reader. Using this method assays can be performed samples of plasma or serum.

Glucose-dependent insulinotropic polypeptide (GIP) is measured by ELISA.

Concentrations of insulin in plasma or serum are determined using an ELISA kit from Millipore.

Concentrations of leptin in plasma or serum are determined using an ELISA kit available from Millipore.

A corticosterone kit from MP Biomedical is used to test the corticosterone levels in plasma. 

A homemade ACTH RIA based on an ACTH antibody (WCE, Univ of MN) and 125I- from MP Biomedical to test the corticosterone levels in plasma.