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Photo of Dorothy M. Supp, PhD

Dorothy M. Supp, PhD

Adjunct Professor

Investigator, Shriners Hospitals for Children - Cincinnati

Surgery | College of Medicine

Education/Credentials
  • Bachelor's Degree: Cornell University, College of Agriculture and Life Sciences
  • Doctoral Degree: University of Cincinnati College of Medicine
  • Fellowship: Childrens Hospital Research Foundation
  • Fellowship: Shriners Hospitals for Children
Contact Information
Research Interests

The purpose of my research is to enhance the performance of cultured skin substitutes for the healing of burns and other skin wounds. Cultured skin substitutes have been used at the Shriners Burns Hospital and the University of Cincinnati as an adjunctive treatment for large burn wounds. Although significant improvements over recent years have led to better clinical outcomes, cultured skin substitutes are still limited by deficiencies in anatomy and physiology compared to native skin autograft. Using the ...

Peer Reviewed Publications

Kim, Jayne Y; Willard, James J; Supp, Dorothy M; Roy, Sashwati; Gordillo, Gayle M; Sen, Chandan K; Powell, Heather M 2015. Burn Scar Biomechanics Following Pressure Garment Therapy. Plastic and reconstructive surgery, ,

Alexander, J Wesley; Supp, Dorothy M 2014. Role of Arginine and Omega-3 Fatty Acids in Wound Healing and Infection. Advances in wound care, 3 11, 682-690

Boyce, Steven T; Zimmerman, Rachel L; Supp, Dorothy M 2014. Tumorigenicity Testing in Athymic Mice of Cultured Human Melanocytes for Transplantation in Engineered Skin Substitutes. Cell transplantation, ,

Osterburg, Andrew R; Hexley, Philip; Supp, Dorothy M; Robinson, Chad T; Noel, Greg; Ogle, Cora; Boyce, Steven T; Aronow, Bruce J; Babcock, George F 2013. Concerns over interspecies transcriptional comparisons in mice and humans after trauma. Proceedings of the National Academy of Sciences of the United States of America, 110 36, E3370

Sriwiriyanont, Penkanok; Lynch, Kaari A; McFarland, Kevin L; Supp, Dorothy M; Boyce, Steven T 2013. Characterization of hair follicle development in engineered skin substitutes. PloS one, 8 6, e65664

Sriwiriyanont, Penkanok; Lynch, Kaari A; Maier, Elizabeth A; Hahn, Jennifer M; Supp, Dorothy M; Boyce, Steven T 2012. Morphogenesis of chimeric hair follicles in engineered skin substitutes with human keratinocytes and murine dermal papilla cells. Experimental dermatology, 21 10, 783-5

Supp, Dorothy M 2012. Genomic Reprogramming and Skin-Like Maturation of Engineered Human Skin Substitutes. Advances in wound care, 1 2, 63-68

Supp, Dorothy M; Glaser, Kathryn; Hahn, Jennifer M; McFarland, Kevin L; Boyce, Steven T 2012. LETTER TO THE EDITOR Abnormal Responses of Keloid Tissue to Wounding Identified Using In Vitro Model System. Eplasty, 12 , e19

Supp, Dorothy M; Hahn, Jennifer M; Glaser, Kathryn; McFarland, Kevin L; Boyce, Steven T 2012. Deep and superficial keloid fibroblasts contribute differentially to tissue phenotype in a novel in vivo model of keloid scar. Plastic and reconstructive surgery, 129 6, 1259-71

Swope, Viki B; Jameson, Joshua A; McFarland, Kevin L; Supp, Dorothy M; Miller, William E; McGraw, Dennis W; Patel, Mira A; Nix, Matthew A; Millhauser, Glenn L; Babcock, George F; Abdel-Malek, Zalfa A 2012. Defining MC1R regulation in human melanocytes by its agonist ?-melanocortin and antagonists agouti signaling protein and ?-defensin 3. The Journal of investigative dermatology, 132 9, 2255-62

Klingenberg, Jennifer M; McFarland, Kevin L; Friedman, Aaron J; Boyce, Steven T; Aronow, Bruce J; Supp, Dorothy M 2010. Engineered human skin substitutes undergo large-scale genomic reprogramming and normal skin-like maturation after transplantation to athymic mice. The Journal of investigative dermatology, 130 2, 587-601

Powell, Heather M; McFarland, Kevin L; Butler, David L; Supp, Dorothy M; Boyce, Steven T 2010. Uniaxial strain regulates morphogenesis, gene expression, and tissue strength in engineered skin. Tissue engineering. Part A, 16 3, 1083-92

Supp, Dorothy M; Gardner, Jason; Klingenberg, Jennifer M; Neely, Alice N 2009. Antibiotic resistance in clinical isolates of Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus does not impact sensitivity to human beta defensin 4. Burns : journal of the International Society for Burn Injuries, 35 7, 949-55

Kalyanaraman, Balaji; Supp, Dorothy M; Boyce, Steven T 2008. Medium flow rate regulates viability and barrier function of engineered skin substitutes in perfusion culture. Tissue engineering. Part A, 14 5, 583-93

Powell, Heather M; Supp, Dorothy M; Boyce, Steven T 2008. Influence of electrospun collagen on wound contraction of engineered skin substitutes. Biomaterials, 29 7, 834-43

Fang, Cheng-Hui; Li, Bingguo; James, J Howard; Yahya, Ayesha; Kadeer, Nijiati; Guo, Xialing; Xiao, Chun; Supp, Dorothy M; Kagan, Richard J; Hasselgren, Per-Olof; Sheriff, Sulaiman 2007. GSK-3beta activity is increased in skeletal muscle after burn injury in rats. American journal of physiology. Regulatory, integrative and comparative physiology, 293 4, R1545-51

Supp, Dorothy M 2007. Gene therapy to fight infection in skin transplants. Pharmacogenomics, 8 5, 483-6

Smiley, Andrea K; Klingenberg, Jennifer M; Boyce, Steven T; Supp, Dorothy M 2006. Keratin expression in cultured skin substitutes suggests that the hyperproliferative phenotype observed in vitro is normalized after grafting. Burns : journal of the International Society for Burn Injuries, 32 2, 135-8

Smiley, Andrea K; Klingenberg, Jennifer M; Aronow, Bruce J; Boyce, Steven T; Kitzmiller, W John; Supp, Dorothy M 2005. Microarray analysis of gene expression in cultured skin substitutes compared with native human skin. The Journal of investigative dermatology, 125 6, 1286-301

Supp, Dorothy M; Karpinski, Andrea C; Boyce, Steven T 2004. Expression of human beta-defensins HBD-1, HBD-2, and HBD-3 in cultured keratinocytes and skin substitutes. Burns : journal of the International Society for Burn Injuries, 30 7, 643-8

Supp, Dorothy M; Wilson-Landy, Kaila; Boyce, Steven T 2002. Human dermal microvascular endothelial cells form vascular analogs in cultured skin substitutes after grafting to athymic mice. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 16 8, 797-804

Supp, D M; Supp, A P; Bell, S M; Boyce, S T 2000. Enhanced vascularization of cultured skin substitutes genetically modified to overexpress vascular endothelial growth factor. The Journal of investigative dermatology, 114 1, 5-13

Boyce S.;Lloyd C.;Kleiner M.;Swope V.;Abdel-Malek Z.;Supp D. 11-01-2017. Restoration of cutaneous pigmentation by transplantation to mice of isogeneic human melanocytes in d Pigment Cell and Melanoma Research, 30 6, 531-540

Hahn J.;Supp D. 11-01-2017. Abnormal expression of the vitamin D receptor in keloid scars Burns, 43 7, 1506-1515

Forouzandeh F.;Jalili R.;Hartwell R.;Allan S.;Boyce S.;Supp D.;Ghahary A. 11-01-2010. Local expression of indoleamine 2,3-dioxygenase suppresses T-cell-mediated rejection of an engineere Wound Repair and Regeneration, 18 6, 614-623

Blackstone B.;Hahn J.;McFarland K.;DeBruler D.;Supp D.;Powell H. 10-15-2018. Inflammatory response and biomechanical properties of coaxial scaffolds for engineered skin in vitro Acta Biomaterialia, 80 , 247-257

Supp D.M.;Hahn J.M.;Combs K.A.;McFarland K.L.;Schwentker A.;Boissy R.E.;Boyce S.T.;Powell H.M.;Lucky A.W. 09-01-2019. Collagen VII Expression Is Required in Both Keratinocytes and Fibroblasts for Anchoring Fibril Forma Cell Transplantation, 28 9-10, 1242-1256

DeBruler D.;Blackstone B.;Baumann M.;McFarland K.;Wulff B.;Wilgus T.;Bailey J.;Supp D.;Powell H. 09-01-2017. Inflammatory responses, matrix remodeling, and re-epithelialization after fractional CO<inf>2& Lasers in Surgery and Medicine, 49 7, 675-685

Lander J.;Supp D.;He H.;Martin L.;Chen X.;Weirauch M.;Boyce S.;Kopan R. 09-01-2017. Analysis of chromatin accessibility in human epidermis identifies putative barrier dysfunction-sensi PLoS ONE, 12 9,

Blackstone B.;Kim J.;McFarland K.;Sen C.;Supp D.;Bailey J.;Powell H. 07-01-2017. Scar formation following excisional and burn injuries in a red Duroc pig model Wound Repair and Regeneration, 25 4, 618-631

Malara M.;Kim J.;Clark J.;Blackstone B.;Ruegsegger M.;Bailey J.;Supp D.;Powell H. 06-13-2018. Structural, Chemical, and Mechanical Properties of Pressure Garments as a Function of Simulated Use Journal of Burn Care and Research, 39 4, 562-571

DeBruler D.;Blackstone B.;McFarland K.;Baumann M.;Supp D.;Bailey J.;Powell H. 06-01-2018. Effect of skin graft thickness on scar development in a porcine burn model Burns, 44 4, 917-930

DeBruler D.;Zbinden J.;Baumann M.;Blackstone B.;Malara M.;Bailey J.;Supp D.;Powell H. 06-01-2018. Early cessation of pressure garment therapy results in scar contraction and thickening PLoS ONE, 13 6,

Kalyanaraman B.;Supp D.;Boyce S. 05-01-2008. Medium flow rate regulates viability and barrier function of engineered skin substitutes in perfusio Tissue Engineering - Part A., 14 5, 583-593

Supp D.;Boyce S. 04-19-2016. Bioengineering of human skin substitutes Tissue Engineering and Artificial Organs, , 715-731

Supp D. 04-01-2011. Skin substitutes for burn wound healing: Current and future approaches Expert Review of Dermatology, 6 2, 217-227

Hahn J.M.;Combs K.A.;Lloyd C.M.;McFarland K.L.;Boyce S.T.;Supp D.M. 03-01-2019. Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting PLoS ONE, 14 3,

Kim J.;Dunham D.;Supp D.;Sen C.;Powell H. 03-01-2016. Novel burn device for rapid, reproducible burn wound generation Burns, 42 2, 384-391

Boyce S.T.;Supp D.M.;Lloyd C.M. 02-01-2020. Exogenous Keratinocyte Growth Factor Is Not Required for Pigmentation of Skin Substitutes with Three Tissue Engineering - Part A, 26 3-4, 214-224

Debruler D.;Baumann M.;Blackstone B.;Zbinden J.;McFarland K.;Bailey J.;Supp D.;Powell H. 02-01-2019. Role of Early Application of Pressure Garments following Burn Injury and Autografting Plastic and Reconstructive Surgery, 143 2, 310e-321e

Supp D. 02-01-2019. Animal Models for Studies of Keloid Scarring Advances in Wound Care, 8 2, 77-89

Abdel-Malek Z.;Supp D. 02-01-2008. ?-Defensin 3: A novel and unexpected key that unlocks the melanocortin 1 receptor Pigment Cell and Melanoma Research, 21 1, 7-8

Supp D.;Neely A. 02-01-2008. Cutaneous antimicrobial gene therapy: Engineering human skin replacements to combat wound infection Expert Review of Dermatology, 3 1, 73-84

Blackstone B.;Malara M.;Baumann M.;McFarland K.;Supp D.;Powell H. 01-15-2020. Fractional CO<inf>2</inf> laser micropatterning of cell-seeded electrospun collagen scaf Acta Biomaterialia, 102 , 287-297

Baumann M.E.;Blackstone B.N.;Malara M.M.;Clairmonte I.A.;Supp D.M.;Bailey J.K.;Powell H.M. 01-01-2019. Fractional CO<inf>2</inf> laser ablation of porcine burn scars after grafting: Is deeper Burns, ,

Bailey J.;Blackstone B.;DeBruler D.;Kim J.;Baumann M.;McFarland K.;Imeokparia F.;Supp D.;Powell H. 01-01-2018. Effects of early combinatorial treatment of autologous split-thickness skin grafts in red duroc pig Lasers in Surgery and Medicine, 50 1, 78-87

Boyce S.;Supp D. 01-01-2016. Biologic Skin Substitutes Skin Tissue Engineering and Regenerative Medicine, , 211-238

Supp D.M.;Hahn J.M.;McFarland K.L.;Combs K.A.;Lee K.S.S.;Inceoglu B.;Wan D.;Boyce S.T.;Hammock B.D. 01-01-2016. Soluble epoxide hydrolase inhibition and epoxyeicosatrienoic acid treatment improve vascularization Plastic and Reconstructive Surgery - Global Open, 4 12,

Swope V.B.;Jameson J.A.;McFarland K.L.;Supp D.M.;Miller W.E.;McGraw D.W.;Patel M.A.;Nix M.A.;Millhauser G.L.;Babcock G.F.;Abdel-Malek Z.A. 01-01-2012. Defining MC1R regulation in human melanocytes by its agonist ?-melanocortin and antagonists agouti s Journal of Investigative Dermatology, 132 9, 2255-2262

Kadekaro A.L.;Leachman S.;Kavanagh R.J.;Swope V.;Cassidy P.;Supp D.;Sartor M.;Schwemberger S.;Babcock G.;Wakamatsu K.;Ito S.;Koshoffer A.;Boissy R.E.;Manga P.;Sturm R.A.;Abdel-Malek Z.A. 01-01-2010. Melanocortin 1 receptor genotype: An important determinant of the damage response of melanocytes to FASEB Journal, 24 10, 3850-3860

Supp D.;Boyce S. 01-01-2007. Bioengineering of human skin substitutes Tissue Engineering, , 275-291

Boyce, Steven T; Rice, Rachel K; Lynch, Kaari A; Supp, Andrew P; Swope, Viki B; Kagan, Richard J; Supp, Dorothy M . Assessment of replication rates of human keratinocytes in engineered skin substitutes grafted to athymic mice. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 20 4, 544-51

Hahn, Jennifer M; Glaser, Kathryn; McFarland, Kevin L; Aronow, Bruce J; Boyce, Steven T; Supp, Dorothy M . Keloid-derived keratinocytes exhibit an abnormal gene expression profile consistent with a distinct causal role in keloid pathology. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 21 4, 530-44

McFarland, Kevin L; Glaser, Kathryn; Hahn, Jennifer M; Boyce, Steven T; Supp, Dorothy M . Culture medium and cell density impact gene expression in normal skin and abnormal scar-derived fibroblasts. Journal of burn care & research : official publication of the American Burn Association, 32 4, 498-508

McFarland, Kevin L; Klingenberg, Jennifer M; Boyce, Steven T; Supp, Dorothy M . Expression of genes encoding antimicrobial proteins and members of the toll-like receptor/nuclear factor-kappaB pathways in engineered human skin. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 16 4, 534-41

Smiley, Andrea K; Gardner, Jason; Klingenberg, Jennifer M; Neely, Alice N; Supp, Dorothy M . Expression of human beta defensin 4 in genetically modified keratinocytes enhances antimicrobial activity. Journal of burn care & research : official publication of the American Burn Association, 28 1, 127-32

Supp, Andrew P; Neely, Alice N; Supp, Dorothy M; Warden, Glenn D; Boyce, Steven T . Evaluation of cytotoxicity and antimicrobial activity of Acticoat Burn Dressing for management of microbial contamination in cultured skin substitutes grafted to athymic mice. The Journal of burn care & rehabilitation, 26 3, 238-46

Supp, D M; Bell, S M; Morgan, J R; Boyce, S T . Genetic modification of cultured skin substitutes by transduction of human keratinocytes and fibroblasts with platelet-derived growth factor-A. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 8 1, 26-35

Supp, Dorothy M; Boyce, Steven T . Engineered skin substitutes: practices and potentials. Clinics in dermatology, 23 4, 403-12

Supp, Dorothy M; Boyce, Steven T . Overexpression of vascular endothelial growth factor accelerates early vascularization and improves healing of genetically modified cultured skin substitutes. The Journal of burn care & rehabilitation, 23 1, 10-20

Supp, Dorothy M; Hahn, Jennifer M; McFarland, Kevin L; Glaser, Kathryn . Inhibition of hyaluronan synthase 2 reduces the abnormal migration rate of keloid keratinocytes. Journal of burn care & research : official publication of the American Burn Association, 35 1, 84-92

Supp, Dorothy M; Karpinski, Andrea C; Boyce, Steven T . Vascular endothelial growth factor overexpression increases vascularization by murine but not human endothelial cells in cultured skin substitutes grafted to athymic mice. The Journal of burn care & rehabilitation, 25 4, 337-45