LSM 710 LIVE Duo Confocal Microscope
Equipped with two separate scanning units, the fast-speed line (LIVE) scanner (up to 120 frames/sec at 512x512) and the LSM710 high-resolution point scanner, integrated into the same microscope. Point Scanner: 6 laser lines (405, 458, 488, 514, 560, 633nm) and 4 detectors (3 spectral confocal fluorescence plus 1 transmitted light detector). LIVE fast line scanner: 3 laser lines (405, 488, 560nm) and 2 confocal fluorescence detectors. The Duo system allows the two scanners to be used in combination for complex, high-speed bleaching or photomanipulation experiments. Temperature-controlled environmental chamber encasing entire microscope.
LSM 510 NLO Two-Photon Microscope
4 lasers (Ti-Sa [700-990 nm; wavelength selection under computer control], Ar [458,477,488,514 nm], Green HeNe [543 nm] and Red HeNe [633 nm]), 6 detectors [3 confocal fluorescence/reflectance, 1 Nomarski transmitted, 2 non-descanned high sensitivity two-photon detectors]). Inverted microscope stage enclosed in light-tight, temperature-controlled environmental chamber. Useful for deep tissue imaging (better than standard confocal) while retaining subcellular resolution in living tissue. The two-photon absorption also allows fluorophore uncaging, cell damage/ablation, spectral shifting, and other photo-catalytic events to be performed with sub-micron resolution in three dimensions.
DMi8 Widefield Fluorescence Microscope
Widefield microscope system equipped with a stage-top incubation chamber for live time-lapse imaging experiments in brightfield and fluorescence. Color camera for histology and phase contrast imaging, and high-sensitivity Hamamatsu ORCA-ER cooled CCD camera for fluorescence imaging. Sola LED light source and 4 fluorescence filters (DAPI, GFP, Texas Red, CY5). Motorized stage enables image stitching of large regions at high magnification.