Today is Tuesday, Sep. 18, 2018

Research

College of Medicine Research Core Facilities

The UC College of Medicine houses a number of research core facilities designated as core service centers. These facilities exist within multiple departments but are collectively supported by the College of Medicine, Office of Research through the Associate Dean for Research Core Facilities: Ken Greis, PhD. (ken.greis@uc.edu; Tel: 515-558-7012).  

The service center designation signifies that the rates charged by each of these facilities have been reviewed and approved by the UC government cost compliance office, thus the service fees can be cross-charged to federal grants and contracts. Details related to the services offered and the internal rates for each of the cores are provided below. Since these rates are substantially subsidized by the University, external investigators should contact individual core directors to get a rate quote.

Resources to offset some of the cost of the core services may be available through a variety of centers and institutes across UC depending on an investigator’s affiliation. Information related to some of those opportunities and the website links are provided below:

 

Close up view of neurons
core

Contact Information

Live Microscopy Core

The Live Microscopy facility is designed to help investigators perform high resolution imaging with either living or fixed specimens. The facility has two advanced Zeiss confocal laser scanning microscopes for use, including one equipped for multiphoton imaging, as well as a Leica DMi8 widefield microscope system, and various stereo and dissection microscopes. Additional equipment available for use are a Laser Capture Microdissection instrument, multimode plate reader, Real-Time-PCR systems, infrared imager, and cryostat.

Location & Hours:
The core is located in Medical Sciences Building Room 3155. It is open 24/7 for approved trained users, or from 9 am to 5 pm, Monday through Friday for technical assistance.

Acknowledgement

Please acknowledge the Live Microscopy Core in any work you publish or present using our facility. This will help us demonstrate the Core’s value to the UC research community and will contribute greatly in our efforts to secure funding for new instruments and services. Please send full citations for your publications to chet.closson@uc.edu so we can accurately track our research contributions. We appreciate your support!

Grant Information

Please contact the core manager to include specific instrumentation and facilities information for your grant submission.

ServiceCost
Confocal/Multiphoton Microscopes: Prime Hours Usage (per hour, Mon-Fri, 9am-5pm)
$35
Confocal/Multiphoton Microscopes: Off Hours Usage (per hour, all times not covered by Prime Hours)
$25
Widefield Microscopes: Prime Hours Usage (per hour, Mon-Fri, 9am-5pm)
$10
Widefield Microscopes: Off Hours Usage (per hour, all times not covered by Prime Hours)
$5
Technician Assistance: Training or Collecting Images (per hour)
$70
ArcturusXT Laser Capture MicroDissection (per hour)
$40
QuantStudio 5 RT-PCR (per plate)
$10
StepOnePlus RT-PCR (per plate)
$10
Li-Cor Odyssey Infrared Imager (unlimited access per year)
$200
Leica Cryostat (unlimited access per year)
$200
Leica Stereo Microscope (unlimited access per year)
$200
PerkinElmer EnVision Multi Mode Plate Reader (unlimited access per year)
$200
LSM 710 LIVE Duo Confocal Microscope

Equipped with two separate scanning units, the fast-speed line (LIVE) scanner (up to 120 frames/sec at 512x512) and the LSM710 high-resolution point scanner, integrated into the same microscope. Point Scanner: 6 laser lines (405, 458, 488, 514, 560, 633nm) and 4 detectors (3 spectral confocal fluorescence plus 1 transmitted light detector). LIVE fast line scanner: 3 laser lines (405, 488, 560nm) and 2 confocal fluorescence detectors. The Duo system allows the two scanners to be used in combination for complex, high-speed bleaching or photomanipulation experiments. Temperature-controlled environmental chamber encasing entire microscope.

Zeiss

LSM 510 NLO Two-Photon Microscope

4 lasers (Ti-Sa [700-990 nm; wavelength selection under computer control], Ar [458,477,488,514 nm], Green HeNe [543 nm] and Red HeNe [633 nm]), 6 detectors [3 confocal fluorescence/reflectance, 1 Nomarski transmitted, 2 non-descanned high sensitivity two-photon detectors]). Inverted microscope stage enclosed in light-tight, temperature-controlled environmental chamber. Useful for deep tissue imaging (better than standard confocal) while retaining subcellular resolution in living tissue. The two-photon absorption also allows fluorophore uncaging, cell damage/ablation, spectral shifting, and other photo-catalytic events to be performed with sub-micron resolution in three dimensions.

Zeiss

DMi8 Widefield Fluorescence Microscope

Widefield microscope system equipped with a stage-top incubation chamber for live time-lapse imaging experiments in brightfield and fluorescence. Color camera for histology and phase contrast imaging, and high-sensitivity Hamamatsu ORCA-ER cooled CCD camera for fluorescence imaging. Sola LED light source and 4 fluorescence filters (DAPI, GFP, Texas Red, CY5). Motorized stage enables image stitching of large regions at high magnification.

Leica