The purpose of the CEG Integrative Technologies Support Core (ITS) is to furnish members with access to state-of-the-art instrumentation and technologies in a manner that enhances overall research effectiveness, cost effectiveness and utilization of time and manpower. In the spirit of “not duplicating services,” the CEG has used a model of providing direct subsidies for members to use technology cores/facilities already existing at the University of Cincinnati and Cincinnati Children’s Hospital Medical Center. These include
- Integrative Health Science Core
- Bioinformatics Core
- Genomics and Microarray Laboratory
- Genotyping Facility
- Proteomics and Metabolomics Laboratory
- High-Field Magnetic-resonance Imaging and Spectroscopy
- Flow Cytometry Core
- Transgenic Mice & Breeding Core
- Analytical & Mass Spectrometry of Small Molecules
Selection and continuation of a core/facility are based on (1) current and anticipated usage, (2) quality of services provided, (3) the technological novelty and the investigative power of the services provided, and (4) consistency with the vision of the CEG to integrate basic, translational and clinical EHS research for the betterment of human health. Cores/facilities may be added or removed based on the these criteria.
In 2015 the CEG invested in a state-of-the-art confocal microscope and immunospot analyzer: The confocal microscope will help investigators generate high-resolution, high quality, microscopy-based images. It is useful for isolation of single cells in conjunction with (prior to/to verify) RNA-sequencing. This machine will be particularly helpful for CEG members wishing to perform single cell and stem cell biology. This equipment is under the direction of Dr. Pheruza Tarapore. Regarding the CTL ImmunoSpot S6 micro analyzer: ImmunoSpot® Analyzers are designed for scanning and evaluating single cell-based spots which reflect the protein expression, function, and growth of cells. The CEG's CTL ImmunoSpot S6 micro analyzer is highly advantageous in functional characterization of rare cells from limited human samples and in the survey of rare subsets from exposure-related epidemiology studies using high-throughput microplate analyses. It is an irreplaceable instrument that can be applied to many CEG-supported studies such as characterization of cytokine production upon environmental stress and determination of microbes from complex microbiota pools. This equipment is under the direction of Dr. Shouxiong Huang.
The majority of the ITS budget is to be used in the form of in-kind subsidies to allow members to leverage NIH/NIEHS- or foundation-funded EHS research. Recipients of CEG Pilot awards can use their awards as matching funds for qualified, subsidized services. The remaining portion of the budget pays for expert advice offered by selected core/facility leaders or key personnel who will provide pre-experimental or pre-grant application consultations.
To apply for an ITS subsidy, please provide all details requested on the application form and submit to the CEG Program Coordinator.
All requests that include use of human subjects or human specimens must include a consultation with the CEG Integrative Health Sciences Core by using the CCTST Research Central portal of the CCTST (Center for Clinical and Translational Science and Training) http://cctst.uc.edu/consult. Under Project Information, Select project affiliation (if applicable) use the dropdown menu to select the Center for Environmental Genetics.
Research supported by the Center for Environmental Genetics should cite NIEHS P30-ES006096