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Department of Environmental Health

Center for Environmental Genetics

Integrative Technologies Support Core

The purpose of the CEG Integrative Technologies Support Core (ITS) is to provide investigators with access to state-of-the-art instrumentation and technologies in a manner that enhances overall research effectiveness, cost effectiveness, and utilization of time and manpower. In the spirit of “not duplicating services,” the CEG has used a model of providing direct subsidies (see below) for members to use technology cores/facilities already existing at the University of Cincinnati and Cincinnati Children’s Hospital Medical Center. These include

  • Genomics, Epigenomics and Sequencing Core (Tier I)
  • Flow Cytometry and Imaging Core (Tier I)
  • Inhalation Core (Tier II)
  • Transgenic Animal and Genome Editing Core (Tier II) (CCHMC)
  • ProteomicsCore (Tier II) (UC)
  • NMR-based Metabolomics Core (Tier II)
  • Animal Behavioral Core (Tier II)
  • Fernald Community Cohort Resources (Tier II)
  • Pluripotent Stem Cell Facility (Tier II) (CCHMC)
  • Metallomics Core (Tier II)

 

Selection, stratification and continuation of a core/facility are based on (1) current and anticipated usage, (2) quality of services provided, (3) the technological novelty and the investigative power of the services provided, and (4) consistency with the vision of the CEG to integrate basic, translational and clinical EHS research for the betterment of human health. Cores/facilities may be added, re-tiered, or removed based on the these criteria.

Tools available via the ITS Core include a state-of-the-art confocal microscope and  immunospot analyzer: The confocal microscope helps investigators to generate high-resolution, high quality, microscopy-based images. It is useful for isolation of single cells in conjunction with (prior to/to verify) RNA-sequencing. This machine is particularly helpful for investigators who wish to perform single cell and stem cell biology. This equipment is under the direction of Dr. Pheruza Tarapore. Regarding the CTL ImmunoSpot S6 micro analyzer: ImmunoSpot® Analyzers are designed for scanning and evaluating single cell-based spots which reflect the protein expression, function, and growth of cells. The CEG's  CTL ImmunoSpot S6 micro analyzer is highly advantageous in functional characterization of rare cells from limited human samples and in the survey of rare subsets from exposure-related epidemiology studies using high-throughput microplate analyses. This instrument, which is under the direction of Dr. Shouxiong Huang, can be applied to many CEG-supported studies such as characterization of cytokine production upon environmental stress and determination of microbes from complex microbiota pools.

The majority of the ITS budget is to be used in the form of in-kind subsidies to allow members to leverage NIH/NIEHS- or foundation-funded EHS research. Recipients of CEG Pilot awards can use their awards as matching funds for qualified, subsidized services. The remaining portion of the budget pays for expert advice offered by selected core/facility leaders or key personnel who will provide pre-experimental or pre-grant application consultations.

To apply for an ITS subsidy, please provide all details requested on the following application form and submit to the CEG Program Coordinator. To download a fillable Word form, click here. PDF version, click here.

All requests that include use of human subjects or human specimens must include a consultation with the CEG Integrative Health Sciences Core by using the Research Central portal of the Center for Clinical and Translational Science and Training: https://cctst.uc.edu. When queried regarding project information, note project affiliation (if applicable) and indicate the Center for Environmental Genetics.

 Research supported by the Center for Environmental Genetics should cite NIEHS P30-ES006096