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Department of

Pharmacology & Systems Physiology 

Photo of Eitaro Aihara, PHD

Eitaro Aihara, PHD

Research Instructor

Research Interests

GI mucosal-defense mechanisms: Our laboratory investigates the mechanisms of GI mucosal defense, including injury repair and regeneration of the epithelium. We utilize advanced two-photon microscopy to visualize repair progression (cell migration) and microenvironment changes, such as luminal pH, intracellular pH, and intracellular calcium both in vivo and in vitro in real time. We are also exploring the effect of Helicobacter pylori and/or specific clinical drugs, including NSAIDs, which have an adverse effect on gastric mucosa during gastric ulcer healing. It is well known that eradication of H. pylori cures gastric ulcers and inhibits recurrence of ulcers in ~80% of patients. Nevertheless, ~20% of patients have an ulcer recurrence, despite successful cure of the H. pylori infection and no reported use of NSAIDs. The question remains whether the site of ulcer healing is the site of ulcer recurrence and source of further diseases, including cancer. Currently, we are investigating molecular mechanisms of ulcer healing and capacity of mucosal defense in the ulcer regenerated epithelium.

  • Doctoral Degree: Kyoto Pharmaceutical University
  • Bachelor's Degree: Kyoto Pharmaceutical University
  • Master's Degree: Kyoto Pharmaceutical University
Contact Information

Peer Reviewed Publications

Aihara, Eitaro; Engevik, Kristen A; Montrose, Marshall H 2017. Trefoil Factor Peptides and Gastrointestinal Function. Annual review of physiology, 79 , 357-380

Asai, Akihiro; Aihara, Eitaro; Watson, Carey; Mourya, Reena; Mizuochi, Tatsuki; Shivakumar, Pranavkumar; Phelan, Kieran; Mayhew, Christopher; Helmrath, Michael; Takebe, Takanori; Wells, James; Bezerra, Jorge A 2017. Paracrine signals regulate human liver organoid maturation from induced pluripotent stem cells. Development (Cambridge, England), 144 6, 1056-1064

McCracken, Kyle W; Aihara, Eitaro; Martin, Baptiste; Crawford, Calyn M; Broda, Taylor; Treguier, Julie; Zhang, Xinghao; Shannon, John M; Montrose, Marshall H; Wells, James M 2017. Wnt/?-catenin promotes gastric fundus specification in mice and humans. Nature, 541 7636, 182-187

Ren, Wenwen; Aihara, Eitaro; Lei, Weiwei; Gheewala, Nishi; Uchiyama, Hironobu; Margolskee, Robert F; Iwatsuki, Ken; Jiang, Peihua 2017. Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation. Scientific reports, 7 1, 4004

Saito, Yuki; Iwatsuki, Ken; Hanyu, Hikaru; Maruyama, Natsuki; Aihara, Eitaro; Tadaishi, Miki; Shimizu, Makoto; Kobayashi-Hattori, Kazuo 2017. Effect of essential amino acids on enteroids: Methionine deprivation suppresses proliferation and affects differentiation in enteroid stem cells. Biochemical and biophysical research communications, 488 1, 171-176

Aihara, Eitaro; Matthis, Andrea L; Karns, Rebekah A; Engevik, Kristen A; Jiang, Peihua; Wang, Jiang; Yacyshyn, Bruce R; Montrose, Marshall H 2016. Epithelial Regeneration After Gastric Ulceration Causes Prolonged Cell-Type Alterations. Cellular and molecular gastroenterology and hepatology, 2 5, 625-647

Engevik, Amy C; Feng, Rui; Choi, Eunyoung; White, Shana; Bertaux-Skeirik, Nina; Li, Jing; Mahe, Maxime M; Aihara, Eitaro; Yang, Li; DiPasquale, Betsy; Oh, Sunghee; Engevik, Kristen A; Giraud, Andrew S; Montrose, Marshall H; Medvedovic, Mario; Helmrath, Michael A; Goldenring, James R; Zavros, Yana 2016. The Development of Spasmolytic Polypeptide/TFF2-Expressing Metaplasia (SPEM) During Gastric Repair Is Absent in the Aged Stomach. Cellular and molecular gastroenterology and hepatology, 2 5, 605-624

Matsu-Ura, Toru; Dovzhenok, Andrey; Aihara, Eitaro; Rood, Jill; Le, Hung; Ren, Yan; Rosselot, Andrew E; Zhang, Tongli; Lee, Choogon; Obrietan, Karl; Montrose, Marshall H; Lim, Sookkyung; Moore, Sean R; Hong, Christian I 2016. Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture. Molecular cell, 64 5, 900-912

Matthis, Andrea L; Zhang, Bin; Denson, Lee A; Yacyshyn, Bruce R; Aihara, Eitaro; Montrose, Marshall H 2016. Importance of the Evaluation of N-Acetyltransferase Enzyme Activity Prior to 5-Aminosalicylic Acid Medication for Ulcerative Colitis. Inflammatory bowel diseases, 22 8, 1793-802

Aihara, Eitaro; Mahe, Maxime M; Schumacher, Michael A; Matthis, Andrea L; Feng, Rui; Ren, Wenwen; Noah, Taeko K; Matsu-ura, Toru; Moore, Sean R; Hong, Christian I; Zavros, Yana; Herness, Scott; Shroyer, Noah F; Iwatsuki, Ken; Jiang, Peihua; Helmrath, Michael A; Montrose, Marshall H 2015. Characterization of stem/progenitor cell cycle using murine circumvallate papilla taste bud organoid. Scientific reports, 5 , 17185

Moore, Sean R; Guedes, Marjorie M; Costa, Tie B; Vallance, Jefferson; Maier, Elizabeth A; Betz, Kristina J; Aihara, Eitaro; Mahe, Maxime M; Lima, Aldo A M; Oriá, Reinaldo B; Shroyer, Noah F 2015. Glutamine and alanyl-glutamine promote crypt expansion and mTOR signaling in murine enteroids. American journal of physiology. Gastrointestinal and liver physiology, 308 10, G831-9

Schumacher, Michael A; Aihara, Eitaro; Feng, Rui; Engevik, Amy; Shroyer, Noah F; Ottemann, Karen M; Worrell, Roger T; Montrose, Marshall H; Shivdasani, Ramesh A; Zavros, Yana 2015. The use of murine-derived fundic organoids in studies of gastric physiology. The Journal of physiology, 593 8, 1809-27

Schumacher, Michael A; Feng, Rui; Aihara, Eitaro; Engevik, Amy C; Montrose, Marshall H; Ottemann, Karen M; Zavros, Yana 2015. Helicobacter pylori-induced Sonic Hedgehog expression is regulated by NF?B pathway activation: the use of a novel in vitro model to study epithelial response to infection. Helicobacter, 20 1, 19-28

Wroblewski, Lydia E; Piazuelo, M Blanca; Chaturvedi, Rupesh; Schumacher, Michael; Aihara, Eitaro; Feng, Rui; Noto, Jennifer M; Delgado, Alberto; Israel, Dawn A; Zavros, Yana; Montrose, Marshall H; Shroyer, Noah; Correa, Pelayo; Wilson, Keith T; Peek, Richard M 2015. Helicobacter pylori targets cancer-associated apical-junctional constituents in gastroids and gastric epithelial cells. Gut, 64 5, 720-30

Aihara, Eitaro; Closson, Chet; Matthis, Andrea L; Schumacher, Michael A; Engevik, Amy C; Zavros, Yana; Ottemann, Karen M; Montrose, Marshall H 2014. Motility and chemotaxis mediate the preferential colonization of gastric injury sites by Helicobacter pylori. PLoS pathogens, 10 7, e1004275

Aihara, Eitaro; Montrose, Marshall H 2014. Importance of Ca(2+) in gastric epithelial restitution-new views revealed by real-time in vivo measurements. Current opinion in pharmacology, 19 , 76-83

Feng, Rui; Aihara, Eitaro; Kenny, Susan; Yang, Li; Li, Jing; Varro, Andrea; Montrose, Marshall H; Shroyer, Noah F; Wang, Timothy C; Shivdasani, Ramesh A; Zavros, Yana 2014. Indian Hedgehog mediates gastrin-induced proliferation in stomach of adult mice. Gastroenterology, 147 3, 655-666.e9

Moore, Sean R; Pruszka, Jill; Vallance, Jefferson; Aihara, Eitaro; Matsuura, Toru; Montrose, Marshall H; Shroyer, Noah F; Hong, Christian I 2014. Robust circadian rhythms in organoid cultures from PERIOD2::LUCIFERASE mouse small intestine. Disease models & mechanisms, 7 9, 1123-30

Ren, Wenwen; Lewandowski, Brian C; Watson, Jaime; Aihara, Eitaro; Iwatsuki, Ken; Bachmanov, Alexander A; Margolskee, Robert F; Jiang, Peihua 2014. Single Lgr5- or Lgr6-expressing taste stem/progenitor cells generate taste bud cells ex vivo. Proceedings of the National Academy of Sciences of the United States of America, 111 46, 16401-6

Aihara, Eitaro; Hentz, Courtney L; Korman, Abraham M; Perry, Nicholas P J; Prasad, Vikram; Shull, Gary E; Montrose, Marshall H 2013. In vivo epithelial wound repair requires mobilization of endogenous intracellular and extracellular calcium. The Journal of biological chemistry, 288 47, 33585-97

Engevik, Melinda A; Aihara, Eitaro; Montrose, Marshall H; Shull, Gary E; Hassett, Daniel J; Worrell, Roger T 2013. Loss of NHE3 alters gut microbiota composition and influences Bacteroides thetaiotaomicron growth. American journal of physiology. Gastrointestinal and liver physiology, 305 10, G697-711

Mahe, Maxime M; Aihara, Eitaro; Schumacher, Michael A; Zavros, Yana; Montrose, Marshall H; Helmrath, Michael A; Sato, Toshiro; Shroyer, Noah F 2013. Establishment of Gastrointestinal Epithelial Organoids. Current protocols in mouse biology, 3 4, 217-40

Demitrack, Elise S; Aihara, Eitaro; Kenny, Susan; Varro, Andrea; Montrose, Marshall H 2012. Inhibitors of acid secretion can benefit gastric wound repair independent of luminal pH effects on the site of damage. Gut, 61 6, 804-11

Xue, Lin; Aihara, Eitaro; Wang, Timothy C; Montrose, Marshall H 2011. Trefoil factor 2 requires Na/H exchanger 2 activity to enhance mouse gastric epithelial repair. The Journal of biological chemistry, 286 44, 38375-82

Xue, Lin; Aihara, Eitaro; Podolsky, Daniel K; Wang, Timothy C; Montrose, Marshall H 2010. In vivo action of trefoil factor 2 (TFF2) to speed gastric repair is independent of cyclooxygenase. Gut, 59 9, 1184-91