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Department of

Molecular Genetics, Biochemistry & Microbiology

Alison Weiss, PhD

Professor of Molecular Genetics, Biochemistry and Microbiology

Education/Credentials
  • A.B.: Washington University
  • M.S.: University of Washington
  • Ph.D.: Stanford University

Research/Clinical Interests

The Weiss laboratory is interested in the molecular mechanisms underlying Bordetella pertussis and E. Coli 0157:H7 pathogenesis. The lab is particularly interested in the actions of the Pertussis and Shiga Toxins encoded by the bacteria. Model systems including organoids are of particular interest.

Contact Information
  • Office 2254 Medical Sciences Building
  • 231 Albert Sabin Way
  • Cincinnati, Ohio 45267
  • Office (513)558-2820
  • Email alison.weiss@uc.edu

Peer Reviewed Publications

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Karve, SS and AA Weiss 2014. Glycolipid Binding Preferences of Shiga Toxin Variants PLoS ONE, 9(7): e101173 ,

Gaston MA, Pellino CA, Weiss AA 2013. Failure of Manganese to Protect from Shiga Toxin. PLoS ONE , 8(7): e69823 ,

Lee, C., M.A. Gaston, A.A. Weiss, and P. Zhang 2013. . Colorimetric viral detection based on sialic acid stabilized gold nanoparticles. Biosensor. Bioelectronic, , 236

Millen SH, Schneider OD, Miller WE, Monaco JJ, Weiss AA 2013. Pertussis Toxin B-Pentamer Mediates Intercellular Transfer of Membrane Proteins and Lipids. PLoS ONE , 8(9): ,

Yosief, H.O., A.A. Weiss and S.S. Iyer 2013. Capture of uropathogenic Escherichia coli using synthetic glycan ligands specific for the Pap-pilus. ChemBioChem, 14 ,

Yosief, H.O., S.S. Iyer, and A.A. Weiss 2013. Binding of Pk-trisaccharide analogs of Globotriaosylceramide to Shiga toxin variants. Infect. Immun, 81 ,

Gallegos, Karen M; Conrady, Deborah G; Karve, Sayali S; Gunasekera, Thusitha S; Herr, Andrew B; Weiss, Alison A 2012. Shiga toxin binding to glycolipids and glycans. PloS one, 7 2, e30368

Schneider, Olivia D; Millen, Scott H; Weiss, Alison A; Miller, William E 2012. Mechanistic insight into pertussis toxin and lectin signaling using T cells engineered to express a CD8?/CD3? chimeric receptor. Biochemistry, 51 20, 4126-37

Archdeacon, Michael T; Kazemi, Namdar; Romanowski, James R; Mobberley-Schuman, Paula S; Weiss, Alison A 2011. Reaming as an adjuvant to irrigation on bacterial presence and propagation: an open, cadaveric intramedullary fracture model. Journal of orthopaedic trauma, 25 6, 325-30

Fuller, Cynthia A; Pellino, Christine A; Flagler, Michael J; Strasser, Jane E; Weiss, Alison A 2011. Shiga toxin subtypes display dramatic differences in potency. Infection and immunity, 79 3, 1329-37

Conrady, Deborah G; Flagler, Michael J; Friedmann, David R; Vander Wielen, Bradley D; Kovall, Rhett A; Weiss, Alison A; Herr, Andrew B 2010. Molecular basis of differential B-pentamer stability of Shiga toxins 1 and 2. PloS one, 5 12, e15153

Flagler, Michael J; Mahajan, Sujit S; Kulkarni, Ashish A; Iyer, Suri S; Weiss, Alison A 2010. Comparison of binding platforms yields insights into receptor binding differences between shiga toxins 1 and 2. Biochemistry, 49 8, 1649-57

Kulkarni, Ashish A; Fuller, Cynthia; Korman, Henry; Weiss, Alison A; Iyer, Suri S 2010. Glycan encapsulated gold nanoparticles selectively inhibit shiga toxins 1 and 2. Bioconjugate chemistry, 21 8, 1486-93

Kulkarni, Ashish A; Weiss, Alison A; Iyer, Suri S 2010. Detection of carbohydrate binding proteins using magnetic relaxation switches. Analytical chemistry, 82 17, 7430-5

Kulkarni, Ashish A; Weiss, Alison A; Iyer, Suri S 2010. Glycan-based high-affinity ligands for toxins and pathogen receptors. Medicinal research reviews, 30 2, 327-93

Millen, Scott H; Lewallen, Daniel M; Herr, Andrew B; Iyer, Suri S; Weiss, Alison A 2010. Identification and characterization of the carbohydrate ligands recognized by pertussis toxin via a glycan microarray and surface plasmon resonance. Biochemistry, 49 28, 5954-67

Schneider, Olivia D; Weiss, Alison A; Miller, William E 2009. Pertussis toxin signals through the TCR to initiate cross-desensitization of the chemokine receptor CXCR4. Journal of immunology (Baltimore, Md. : 1950), 182 9, 5730-9

Conrady, Deborah G; Brescia, Cristin C; Horii, Katsunori; Weiss, Alison A; Hassett, Daniel J; Herr, Andrew B 2008. A zinc-dependent adhesion module is responsible for intercellular adhesion in staphylococcal biofilms. Proceedings of the National Academy of Sciences of the United States of America, 105 49, 19456-61

Hatch, Duane M; Weiss, Alison A; Kale, Ramesh R; Iyer, Suri S 2008. Biotinylated bi- and tetra-antennary glycoconjugates for Escherichia coli detection. Chembiochem : a European journal of chemical biology, 9 15, 2433-42

Kale, Ramesh R; McGannon, Colleen M; Fuller-Schaefer, Cynthia; Hatch, Duane M; Flagler, Michael J; Gamage, Shantini D; Weiss, Alison A; Iyer, Suri S 2008. Differentiation between structurally homologous Shiga 1 and Shiga 2 toxins by using synthetic glycoconjugates. Angewandte Chemie (International ed. in English), 47 7, 1265-8

Taft, Sarah C; Weiss, Alison A 2008. Toxicity of anthrax toxin is influenced by receptor expression. Clinical and vaccine immunology : CVI, 15 9, 1330-6

Taft, Sarah C; Weiss, Alison A 2008. Neutralizing activity of vaccine-induced antibodies to two Bacillus anthracis toxin components, lethal factor and edema factor. Clinical and vaccine immunology : CVI, 15 1, 71-5

Flagler, Michael J; Strasser, Jane E; Chalk, Claudia L; Weiss, Alison A 2007. Comparative analysis of the abilities of Shiga toxins 1 and 2 to bind to and influence neutrophil apoptosis. Infection and immunity, 75 2, 760-5

Schneider, Olivia D; Weiss, Alison A; Miller, William E 2007. Pertussis toxin utilizes proximal components of the T-cell receptor complex to initiate signal transduction events in T cells. Infection and immunity, 75 8, 4040-9

Gamage, Shantini D; Patton, Angela K; Strasser, Jane E; Chalk, Claudia L; Weiss, Alison A 2006. Commensal bacteria influence Escherichia coli O157:H7 persistence and Shiga toxin production in the mouse intestine. Infection and immunity, 74 3, 1977-83

Hanson, James F; Taft, Sarah C; Weiss, Alison A 2006. Neutralizing antibodies and persistence of immunity following anthrax vaccination. Clinical and vaccine immunology : CVI, 13 2, 208-13

Watanabe, Mineo; Connelly, Beverly; Weiss, Alison A 2006. Characterization of serological responses to pertussis. Clinical and vaccine immunology : CVI, 13 3, 341-8

Mobberley-Schuman, Paula S; Weiss, Alison A 2005. Influence of CR3 (CD11b/CD18) expression on phagocytosis of Bordetella pertussis by human neutrophils. Infection and immunity, 73 11, 7317-23

Gamage, Shantini D; McGannon, Colleen M; Weiss, Alison A 2004. Escherichia coli serogroup O107/O117 lipopolysaccharide binds and neutralizes Shiga toxin 2. Journal of bacteriology, 186 16, 5506-12

Gamage, Shantini D; Patton, Angela K; Hanson, James F; Weiss, Alison A 2004. Diversity and host range of Shiga toxin-encoding phage. Infection and immunity, 72 12, 7131-9

Millen, Scott H; Bernstein, David I; Connelly, Beverly; Ward, Joel I; Chang, Swei-Ju; Weiss, Alison A 2004. Antibody-mediated neutralization of pertussis toxin-induced mitogenicity of human peripheral blood mononuclear cells. Infection and immunity, 72 1, 615-20

Rambow-Larsen, Amy A; Weiss, Alison A 2004. Temporal expression of pertussis toxin and Ptl secretion proteins by Bordetella pertussis. Journal of bacteriology, 186 1, 43-50

Schaeffer, Lyndsay M; McCormack, Francis X; Wu, Huixing; Weiss, Alison A 2004. Interactions of pulmonary collectins with Bordetella bronchiseptica and Bordetella pertussis lipopolysaccharide elucidate the structural basis of their antimicrobial activities. Infection and immunity, 72 12, 7124-30

Schaeffer, Lyndsay M; McCormack, Francis X; Wu, Huixing; Weiss, Alison A 2004. Bordetella pertussis lipopolysaccharide resists the bactericidal effects of pulmonary surfactant protein A. Journal of immunology (Baltimore, Md. : 1950), 173 3, 1959-65

Weiss, Alison A; Patton, Angela K; Millen, Scott H; Chang, Swei-Ju; Ward, Joel I; Bernstein, David I 2004. Acellular pertussis vaccines and complement killing of Bordetella pertussis. Infection and immunity, 72 12, 7346-51

Barnes, Michael G; Weiss, Alison A 2003. Activation of the complement cascade by Bordetella pertussis. FEMS microbiology letters, 220 2, 271-5

Gamage, Shantini D; Strasser, Jane E; Chalk, Claudia L; Weiss, Alison A 2003. Nonpathogenic Escherichia coli can contribute to the production of Shiga toxin. Infection and immunity, 71 6, 3107-15

Mobberley-Schuman, Paula S; Connelly, Beverly; Weiss, Alison A 2003. Phagocytosis of Bordetella pertussis incubated with convalescent serum. The Journal of infectious diseases, 187 10, 1646-53

Stenson, Trevor H; Patton, Angela K; Weiss, Alison A 2003. Reduced glutathione is required for pertussis toxin secretion by Bordetella pertussis. Infection and immunity, 71 3, 1316-20

Barnes, Michael G; Weiss, Alison A 2002. Growth phase influences complement resistance of Bordetella pertussis. Infection and immunity, 70 1, 403-6

Rambow-Larsen, Amy A; Weiss, Alison A 2002. The PtlE protein of Bordetella pertussis has peptidoglycanase activity required for Ptl-mediated pertussis toxin secretion. Journal of bacteriology, 184 11, 2863-9

Stenson, Trevor H; Weiss, Alison A 2002. DsbA and DsbC are required for secretion of pertussis toxin by Bordetella pertussis. Infection and immunity, 70 5, 2297-303

Barnes, M G; Weiss, A A 2001. BrkA protein of Bordetella pertussis inhibits the classical pathway of complement after C1 deposition. Infection and immunity, 69 5, 3067-72

Schaeffer, L M; Weiss, A A 2001. Pertussis toxin and lipopolysaccharide influence phagocytosis of Bordetella pertussis by human monocytes. Infection and immunity, 69 12, 7635-41

Craig-Mylius, K A; Stenson, T H; Weiss, A A 2000. Mutations in the S1 subunit of pertussis toxin that affect secretion. Infection and immunity, 68 3, 1276-81

Craig-Mylius, K A; Weiss, A A 2000. Antibacterial agents and release of periplasmic pertussis toxin from Bordetella pertussis. Antimicrobial agents and chemotherapy, 44 5, 1383-6

Lenz, D H; Weingart, C L; Weiss, A A 2000. Phagocytosed Bordetella pertussis fails to survive in human neutrophils. Infection and immunity, 68 2, 956-9

Weingart, C L; Keitel, W A; Edwards, K M; Weiss, A A 2000. Characterization of bactericidal immune responses following vaccination with acellular pertussis vaccines in adults. Infection and immunity, 68 12, 7175-9

Weingart, C L; Mobberley-Schuman, P S; Hewlett, E L; Gray, M C; Weiss, A A 2000. Neutralizing antibodies to adenylate cyclase toxin promote phagocytosis of Bordetella pertussis by human neutrophils. Infection and immunity, 68 12, 7152-5

Weingart, C L; Weiss, A A 2000. Bordetella pertussis virulence factors affect phagocytosis by human neutrophils. Infection and immunity, 68 3, 1735-9

Craig-Mylius, K A; Weiss, A A 1999. Mutants in the ptlA-H genes of Bordetella pertussis are deficient for pertussis toxin secretion. FEMS microbiology letters, 179 2, 479-84

Weingart, C L; Broitman-Maduro, G; Dean, G; Newman, S; Peppler, M; Weiss, A A 1999. Fluorescent labels influence phagocytosis of Bordetella pertussis by human neutrophils. Infection and immunity, 67 8, 4264-7

Weiss, A A; Mobberley, P S; Fernandez, R C; Mink, C M 1999. Characterization of human bactericidal antibodies to Bordetella pertussis. Infection and immunity, 67 3, 1424-31

Fernandez, R C; Weiss, A A 1998. Serum resistance in bvg-regulated mutants of Bordetella pertussis. FEMS microbiology letters, 163 1, 57-63

Rambow, A A; Fernandez, R C; Weiss, A A 1998. Characterization of BrkA expression in Bordetella bronchiseptica. Infection and immunity, 66 8, 3978-80

Fernandez, R C; Weiss, A A 1996. Susceptibilities of Bordetella pertussis strains to antimicrobial peptides. Antimicrobial agents and chemotherapy, 40 4, 1041-3

Fernandez, R C; Weiss, A A 1995. Cloning and sequencing of the Bordetella pertussis cpn10/cpn60 (groESL) homolog. Gene, 158 1, 151-2

Fernandez, R C; Weiss, A A 1994. Cloning and sequencing of a Bordetella pertussis serum resistance locus. Infection and immunity, 62 11, 4727-38

Walker, K E; Weiss, A A 1994. Characterization of the dermonecrotic toxin in members of the genus Bordetella. Infection and immunity, 62 9, 3817-28

Hewlett, E L; Gray, M C; Ehrmann, I E; Maloney, N J; Otero, A S; Gray, L; Allietta, M; Szabo, G; Weiss, A A; Barry, E M 1993. Characterization of adenylate cyclase toxin from a mutant of Bordetella pertussis defective in the activator gene, cyaC. The Journal of biological chemistry, 268 11, 7842-8

Ehrmann, I E; Weiss, A A; Goodwin, M S; Gray, M C; Barry, E; Hewlett, E L 1992. Enzymatic activity of adenylate cyclase toxin from Bordetella pertussis is not required for hemolysis. FEBS letters, 304 1, 51-6

Barry, E M; Weiss, A A; Ehrmann, I E; Gray, M C; Hewlett, E L; Goodwin, M S 1991. Bordetella pertussis adenylate cyclase toxin and hemolytic activities require a second gene, cyaC, for activation. Journal of bacteriology, 173 2, 720-6

Stibitz, S; Weiss, A A; Falkow, S 1988. Genetic analysis of a region of the Bordetella pertussis chromosome encoding filamentous hemagglutinin and the pleiotropic regulatory locus vir. Journal of bacteriology, 170 7, 2904-13

Pearson, R D; Symes, P; Conboy, M; Weiss, A A; Hewlett, E L 1987. Inhibition of monocyte oxidative responses by Bordetella pertussis adenylate cyclase toxin. Journal of immunology (Baltimore, Md. : 1950), 139 8, 2749-54

Cronin, M J; Evans, W S; Rogol, A D; Weiss, A A; Thorner, M O; Orth, D N; Nicholson, W E; Yasumoto, T; Hewlett, E L 1986. Prokaryotic adenylate cyclase toxin stimulates anterior pituitary cells in culture. The American journal of physiology, 251 2 Pt 1, E164-71

Weiss, A A; Hewlett, E L 1986. Virulence factors of Bordetella pertussis. Annual review of microbiology, 40 , 661-86

Hewlett, E L; Weiss, A A; Crane, J K; Pearson, R D; Anderson, H J; Myers, G A; Evans, W S; Hantske, L L; Kay, H D; Cronin, M J 1985. Bordetella extracytoplasmic adenylate cyclase: actions as a bacterial toxin. Developments in biological standardization, 61 , 21-6

Steinman, L; Weiss, A; Adelman, N; Lim, M; Oehlert, J; Zuniga, R; Hewlett, E; Falkow, S 1985. Murine model for pertussis vaccine encephalopathy: role of the major histocompatibility complex; antibody to albumin and to Bordetella pertussis and pertussis toxin. Developments in biological standardization, 61 , 439-46

Steinman, L; Weiss, A; Adelman, N; Lim, M; Zuniga, R; Oehlert, J; Hewlett, E; Falkow, S 1985. Pertussis toxin is required for pertussis vaccine encephalopathy. Proceedings of the National Academy of Sciences of the United States of America, 82 24, 8733-6

Weiss, A A; Hewlett, E L; Myers, G A; Falkow, S 1985. Genetic studies of the molecular basis of whooping cough. Developments in biological standardization, 61 , 11-9

Weiss, A A; Falkow, S 1984. Genetic analysis of phase change in Bordetella pertussis. Infection and immunity, 43 1, 263-9

Weiss, A A; Hewlett, E L; Myers, G A; Falkow, S 1984. Pertussis toxin and extracytoplasmic adenylate cyclase as virulence factors of Bordetella pertussis. The Journal of infectious diseases, 150 2, 219-22

Weiss, A A; Falkow, S 1983. Transposon insertion and subsequent donor formation promoted by Tn501 in Bordetella pertussis. Journal of bacteriology, 153 1, 304-9

Weiss, A A; Hewlett, E L; Myers, G A; Falkow, S 1983. Tn5-induced mutations affecting virulence factors of Bordetella pertussis. Infection and immunity, 42 1, 33-41

Weiss, A A; Falkow, S 1982. Plasmid transfer to Bordetella pertussis: conjugation and transformation. Journal of bacteriology, 152 1, 549-52