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Department of

Molecular Genetics, Biochemistry & Microbiology

Photo of  Tom Thompson, PhD

Tom Thompson, PhD

Professor of Molecular Genetics

Education/Credentials
  • B.S.: Northern Illinois University
  • M.S.: Northern Illinois University
  • PhD: University of Wisconsin
  • Post Doc: Nortwestern University

Research/Clinical Interests

The focus of my laboratory is divided into two areas of investigation where I study the structural and functional aspects of TGFbeta family signaling and regulation along with the structures of apolipoproteins and how this relates to HDL particles and other related biological functions. The laboratory uses a combination of structural techniques including X-ray crstallography, small angle X-ray scattering coupled with biophysical and biochemical experiments. For more information on the Thompson lab please click here

Contact Information
  • Office 2204 Medical Sciences Building
  • 231 Albert Sabin Way
  • Cincinnati, Ohio 45267
  • Office 513-558-4517
  • Fax 513-558-5604
  • Email Tom.Thompson@uc.edu

Peer Reviewed Publications

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Cotton, Thomas R; Fischer, Gerhard; Wang, Xuelu; McCoy, Jason C; Czepnik, Magdalena; Thompson, Thomas B; Hyvönen, Marko 2018. Structure of the human myostatin precursor and determinants of growth factor latency. The EMBO journal, 37 3, 367-383

Walker, Ryan G; McCoy, Jason C; Czepnik, Magdalena; Mills, Melanie J; Hagg, Adam; Walton, Kelly L; Cotton, Thomas R; Hyvönen, Marko; Lee, Richard T; Gregorevic, Paul; Harrison, Craig A; Thompson, Thomas B 2018. Molecular characterization of latent GDF8 reveals mechanisms of activation. Proceedings of the National Academy of Sciences of the United States of America, 115 5, E866-E875

Bylund, Jeffery B; Trinh, Linh T; Awgulewitsch, Cassandra P; Paik, David T; Jetter, Christopher; Jha, Rajneesh; Zhang, Jianhua; Nolan, Kristof; Xu, Chunhui; Thompson, Thomas B; Kamp, Timothy J; Hatzopoulos, Antonis K 2017. Coordinated Proliferation and Differentiation of Human-Induced Pluripotent Stem Cell-Derived Cardiac Progenitor Cells Depend on Bone Morphogenetic Protein Signaling Regulation by GREMLIN 2. Stem cells and development, 26 9, 678-693

Kattamuri, Chandramohan; Nolan, Kristof; Thompson, Thomas B 2017. Analysis and identification of the Grem2 heparin/heparan sulfate-binding motif. The Biochemical journal, 474 7, 1093-1107

Kattamuri, Chandramohan; Nolan, Kristof; Thompson, Thomas B 2017. Correction: Analysis and identification of the Grem2 heparin/heparan sulfate-binding motif. The Biochemical journal, 474 9, 1529

Melchior, John T; Walker, Ryan G; Cooke, Allison L; Morris, Jamie; Castleberry, Mark; Thompson, Thomas B; Jones, Martin K; Song, Hyun D; Rye, Kerry-Anne; Oda, Michael N; Sorci-Thomas, Mary G; Thomas, Michael J; Heinecke, Jay W; Mei, Xiaohu; Atkinson, David; Segrest, Jere P; Lund-Katz, Sissel; Phillips, Michael C; Davidson, W Sean 2017. A consensus model of human apolipoprotein A-I in its monomeric and lipid-free state. Nature structural & molecular biology, 24 12, 1093-1099

Walker, Ryan G; Czepnik, Magdalena; Goebel, Erich J; McCoy, Jason C; Vujic, Ana; Cho, Miook; Oh, Juhyun; Aykul, Senem; Walton, Kelly L; Schang, Gauthier; Bernard, Daniel J; Hinck, Andrew P; Harrison, Craig A; Martinez-Hackert, Erik; Wagers, Amy J; Lee, Richard T; Thompson, Thomas B 2017. Structural basis for potency differences between GDF8 and GDF11. BMC biology, 15 1, 19

Li, Naisi; Yang, Qiyuan; Walker, Ryan G; Thompson, Thomas B; Du, Min; Rodgers, Buel D 2016. Myostatin Attenuation In Vivo Reduces Adiposity, but Activates Adipogenesis. Endocrinology, 157 1, 282-91

Melchior, John T; Walker, Ryan G; Morris, Jamie; Jones, Martin K; Segrest, Jere P; Lima, Diogo B; Carvalho, Paulo C; Gozzo, Fábio C; Castleberry, Mark; Thompson, Thomas B; Davidson, W Sean 2016. An Evaluation of the Crystal Structure of C-terminal Truncated Apolipoprotein A-I in Solution Reveals Structural Dynamics Related to Lipid Binding. The Journal of biological chemistry, 291 10, 5439-51

Nolan, Kristof; Kattamuri, Chandramohan; Rankin, Scott A; Read, Randy J; Zorn, Aaron M; Thompson, Thomas B 2016. Structure of Gremlin-2 in Complex with GDF5 Gives Insight into DAN-Family-Mediated BMP Antagonism. Cell reports, 16 8, 2077-86

Poggioli, Tommaso; Vujic, Ana; Yang, Peiguo; Macias-Trevino, Claudio; Uygur, Aysu; Loffredo, Francesco S; Pancoast, James R; Cho, Miook; Goldstein, Jill; Tandias, Rachel M; Gonzalez, Emilia; Walker, Ryan G; Thompson, Thomas B; Wagers, Amy J; Fong, Yick W; Lee, Richard T 2016. Circulating Growth Differentiation Factor 11/8 Levels Decline With Age. Circulation research, 118 1, 29-37

Sanders, Lehanna N; Schoenhard, John A; Saleh, Mohamed A; Mukherjee, Amrita; Ryzhov, Sergey; McMaster, William G; Nolan, Kristof; Gumina, Richard J; Thompson, Thomas B; Magnuson, Mark A; Harrison, David G; Hatzopoulos, Antonis K 2016. BMP Antagonist Gremlin 2 Limits Inflammation After Myocardial Infarction. Circulation research, 119 3, 434-49

Walker, Ryan G; Poggioli, Tommaso; Katsimpardi, Lida; Buchanan, Sean M; Oh, Juhyun; Wattrus, Sam; Heidecker, Bettina; Fong, Yick W; Rubin, Lee L; Ganz, Peter; Thompson, Thomas B; Wagers, Amy J; Lee, Richard T 2016. Biochemistry and Biology of GDF11 and Myostatin: Similarities, Differences, and Questions for Future Investigation. Circulation research, 118 7, 1125-42

Yilmaz, Atilgan; Kattamuri, Chandramohan; Ozdeslik, Rana N; Schmiedel, Carolyn; Mentzer, Sarah; Schorl, Christoph; Oancea, Elena; Thompson, Thomas B; Fallon, Justin R 2016. MuSK is a BMP co-receptor that shapes BMP responses and calcium signaling in muscle cells. Science signaling, 9 444, ra87

Deng, Xiaodi; Walker, Ryan G; Morris, Jamie; Davidson, W Sean; Thompson, Thomas B 2015. Role of Conserved Proline Residues in Human Apolipoprotein A-IV Structure and Function. The Journal of biological chemistry, 290 17, 10689-702

Nolan, Kristof; Kattamuri, Chandramohan; Luedeke, David M; Angerman, Elizabeth B; Rankin, Scott A; Stevens, Mariana L; Zorn, Aaron M; Thompson, Thomas B 2015. Structure of neuroblastoma suppressor of tumorigenicity 1 (NBL1): insights for the functional variability across bone morphogenetic protein (BMP) antagonists. The Journal of biological chemistry, 290 8, 4759-71

Walker, Ryan G; Angerman, Elizabeth B; Kattamuri, Chandramohan; Lee, Yun-Sil; Lee, Se-Jin; Thompson, Thomas B 2015. Alternative binding modes identified for growth and differentiation factor-associated serum protein (GASP) family antagonism of myostatin. The Journal of biological chemistry, 290 12, 7506-16

Walker, Ryan G; Thompson, Thomas B 2015. Fibronectin-based scaffold domain proteins that bind myostatin: a patent evaluation of WO2014043344. Expert opinion on therapeutic patents, 25 5, 619-24

Nolan, Kristof; Thompson, Thomas B 2014. The DAN family: Modulators of TGF-? signaling and beyond. Protein science : a publication of the Protein Society, ,

Peng, Jia; Wigglesworth, Karen; Rangarajan, Adithya; Eppig, John J; Thompson, Thomas B; Matzuk, Martin M 2014. Amino acid 72 of mouse and human GDF9 mature domain is responsible for altered homodimer bioactivities but has subtle effects on GDF9:BMP15 heterodimer activities. Biology of reproduction, 91 6, 142

Rodgers, Buel D; Wiedeback, Benjamin D; Hoversten, Knut E; Jackson, Melissa F; Walker, Ryan G; Thompson, Thomas B 2014. Myostatin stimulates, not inihibits, C2C12 myoblast proliferation. Endocrinology, 155 3, 670-5

Walker, Ryan G; Deng, Xiaodi; Melchior, John T; Morris, Jamie; Tso, Patrick; Jones, Martin K; Segrest, Jere P; Thompson, Thomas B; Davidson, W Sean 2014. The structure of human apolipoprotein A-IV as revealed by stable isotope-assisted cross-linking, molecular dynamics, and small angle x-ray scattering. The Journal of biological chemistry, 289 9, 5596-608

Cash, Jennifer N; Angerman, Elizabeth B; Kirby, R Jason; Merck, Lisa; Seibel, William L; Wortman, Matthew D; Papoian, Ruben; Nelson, Sandra; Thompson, Thomas B 2013. Development of a Small-Molecule Screening Method for Inhibitors of Cellular Response to Myostatin and Activin A. Journal of biomolecular screening, ,

Deng, Xiaodi; Morris, Jamie; Chaton, Catherine; Schröder, Gunnar F; Davidson, W Sean; Thompson, Thomas B 2013. Small-angle X-ray scattering of apolipoprotein A-IV reveals the importance of its termini for structural stability. The Journal of biological chemistry, 288 7, 4854-66

Nolan, Kristof; Kattamuri, Chandramohan; Luedeke, David M; Deng, Xiaodi; Jagpal, Amrita; Zhang, Fuming; Linhardt, Robert J; Kenny, Alan P; Zorn, Aaron M; Thompson, Thomas B 2013. Structure of Protein Related to Dan and Cerberus: Insights into the Mechanism of Bone Morphogenetic Protein Antagonism. Structure (London, England : 1993), ,

Peng, Jia; Li, Qinglei; Wigglesworth, Karen; Rangarajan, Adithya; Kattamuri, Chandramohan; Peterson, Randall T; Eppig, John J; Thompson, Thomas B; Matzuk, Martin M 2013. Growth differentiation factor 9:bone morphogenetic protein 15 heterodimers are potent regulators of ovarian functions. Proceedings of the National Academy of Sciences of the United States of America, 110 8, E776-85

Peng, Jia; Li, Qinglei; Wigglesworth, Karen; Rangarajan, Adithya; Kattamuri, Chandramohan; Peterson, Randall T; Eppig, John J; Thompson, Thomas B; Matzuk, Martin M 2013. Reply to Mottershead et al.: GDF9:BMP15 heterodimers are potent regulators of ovarian functions. Proceedings of the National Academy of Sciences of the United States of America, 110 25, E2258

Rejon, Carlis A; Hancock, Mark A; Li, Yining N; Thompson, Thomas B; Hébert, Terence E; Bernard, Daniel J 2013. Activins bind and signal via bone morphogenetic protein receptor type II (BMPR2) in immortalized gonadotrope-like cells. Cellular signalling, 25 12, 2717-26

Cash, Jennifer N; Angerman, Elizabeth B; Kattamuri, Chandramohan; Nolan, Kristof; Zhao, Huaying; Sidis, Yisrael; Keutmann, Henry T; Thompson, Thomas B 2012. Structure of myostatin·follistatin-like 3: N-terminal domains of follistatin-type molecules exhibit alternate modes of binding. The Journal of biological chemistry, 287 2, 1043-53

Cash, Jennifer N; Angerman, Elizabeth B; Keutmann, Henry T; Thompson, Thomas B 2012. Characterization of follistatin-type domains and their contribution to myostatin and activin a antagonism. Molecular endocrinology (Baltimore, Md.), 26 7, 1167-78

Deng, Xiaodi; Davidson, W Sean; Thompson, Thomas B 2012. Improving the diffraction of apoA-IV crystals through extreme dehydration. Acta crystallographica. Section F, Structural biology and crystallization communications, 68 Pt 1, 105-10

Deng, Xiaodi; Morris, Jamie; Dressmen, James; Tubb, Matthew R; Tso, Patrick; Jerome, W Gray; Davidson, W Sean; Thompson, Thomas B 2012. The structure of dimeric apolipoprotein A-IV and its mechanism of self-association. Structure (London, England : 1993), 20 5, 767-79

Kattamuri, Chandramohan; Luedeke, David M; Nolan, Kristof; Rankin, Scott A; Greis, Kenneth D; Zorn, Aaron M; Thompson, Thomas B 2012. Members of the DAN Family Are BMP Antagonists That Form Highly Stable Noncovalent Dimers. Journal of molecular biology, 424 5, 313-27

Kattamuri, Chandramohan; Luedeke, David M; Thompson, Thomas B 2012. Expression and purification of recombinant protein related to DAN and cerberus (PRDC). Protein expression and purification, 82 2, 389-95

Zhang, Fuming; Beaudet, Julie M; Luedeke, David M; Walker, Ryan G; Thompson, Thomas B; Linhardt, Robert J 2012. Analysis of the interaction between heparin and follistatin and heparin and follistatin-ligand complexes using surface plasmon resonance. Biochemistry, 51 34, 6797-803

Syed, Khajamohiddin; Kattamuri, Chandramohan; Thompson, Thomas B; Yadav, Jagjit S 2011. Cytochrome b? reductase-cytochrome b? as an active P450 redox enzyme system in Phanerochaete chrysosporium: atypical properties and in vivo evidence of electron transfer capability to CYP63A2. Archives of biochemistry and biophysics, 509 1, 26-32

Cash, Jennifer N; Rejon, Carlis A; McPherron, Alexandra C; Bernard, Daniel J; Thompson, Thomas B 2009. The structure of myostatin:follistatin 288: insights into receptor utilization and heparin binding. The EMBO journal, 28 17, 2662-76

Stamler, Robin; Keutmann, Henry T; Sidis, Yisrael; Kattamuri, Chandramohan; Schneyer, Alan; Thompson, Thomas B 2008. The structure of FSTL3.activin A complex. Differential binding of N-terminal domains influences follistatin-type antagonist specificity. The Journal of biological chemistry, 283 47, 32831-8

Davidson, W Sean; Thompson, Thomas B 2007. The structure of apolipoprotein A-I in high density lipoproteins. The Journal of biological chemistry, 282 31, 22249-53

Cook, Robert W; Thompson, Thomas B; Kurup, Sudhi P; Jardetzky, Theodore S; Woodruff, Teresa K 2005. Structural basis for a functional antagonist in the transforming growth factor beta superfamily. The Journal of biological chemistry, 280 48, 40177-86

Thompson, Thomas B; Lerch, Thomas F; Cook, Robert W; Woodruff, Teresa K; Jardetzky, Theodore S 2005. The structure of the follistatin:activin complex reveals antagonism of both type I and type II receptor binding. Developmental cell, 9 4, 535-43

Yuan, Ping; Thompson, Thomas B; Wurzburg, Beth A; Paterson, Reay G; Lamb, Robert A; Jardetzky, Theodore S 2005. Structural studies of the parainfluenza virus 5 hemagglutinin-neuraminidase tetramer in complex with its receptor, sialyllactose. Structure (London, England : 1993), 13 5, 803-15

Cook, Robert W; Thompson, Thomas B; Jardetzky, Theodore S; Woodruff, Teresa K 2004. Molecular biology of inhibin action. Seminars in reproductive medicine, 22 3, 269-76

Thompson, Thomas B; Cook, Robert W; Chapman, Stacey C; Jardetzky, Theodore S; Woodruff, Teresa K 2004. Beta A versus beta B: is it merely a matter of expression? Molecular and cellular endocrinology, 225 1-2, 9-17

Thompson, Thomas B; Katayama, Kinya; Watanabe, Kenji; Hutchinson, C Richard; Rayment, Ivan 2004. Structural and functional analysis of tetracenomycin F2 cyclase from Streptomyces glaucescens. A type II polyketide cyclase. The Journal of biological chemistry, 279 36, 37956-63

Thompson, Thomas B; Woodruff, Teresa K; Jardetzky, Theodore S 2003. Structures of an ActRIIB:activin A complex reveal a novel binding mode for TGF-beta ligand:receptor interactions. The EMBO journal, 22 7, 1555-66

Bauer, C B; Fonseca, M V; Holden, H M; Thoden, J B; Thompson, T B; Escalante-Semerena, J C; Rayment, I 2001. Three-dimensional structure of ATP:corrinoid adenosyltransferase from Salmonella typhimurium in its free state, complexed with MgATP, or complexed with hydroxycobalamin and MgATP. Biochemistry, 40 2, 361-74

Thomas, M G; Thompson, T B; Rayment, I; Escalante-Semerena, J C 2000. Analysis of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) enzyme of Salmonella typhimurium LT2. Identification of residue His-46 as the site of guanylylation. The Journal of biological chemistry, 275 36, 27576-86

Thompson, T B; Garrett, J B; Taylor, E A; Meganathan, R; Gerlt, J A; Rayment, I 2000. Evolution of enzymatic activity in the enolase superfamily: structure of o-succinylbenzoate synthase from Escherichia coli in complex with Mg2+ and o-succinylbenzoate. Biochemistry, 39 35, 10662-76

Thompson, T B; Thomas, M G; Escalante-Semerena, J C; Rayment, I 1999. Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) complexed with GMP: evidence for a substrate-induced transferase active site. Biochemistry, 38 40, 12995-3005

Thompson, T B; Thomas, M G; Escalante-Semerena, J C; Rayment, I 1998. Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium determined to 2.3 A resolution,. Biochemistry, 37 21, 7686-95

Fisher, A J; Thompson, T B; Thoden, J B; Baldwin, T O; Rayment, I 1996. The 1.5-A resolution crystal structure of bacterial luciferase in low salt conditions. The Journal of biological chemistry, 271 36, 21956-68

Thompson, T B; Chou, K C; Zheng, C 1995. Neural network prediction of the HIV-1 protease cleavage sites. Journal of theoretical biology, 177 4, 369-79

Thompson, T B; Chou, K C; Zheng, C 1995. Analysis of the loop-helix interaction in bundle motif protein structures. Journal of protein chemistry, 14 7, 559-66