Our laboratory conducts basic research on muscle physiology at the molecular and cellular levels.
Recent research projects have focused on:
- the physiological roles and regulation of the Na, K-ATPase α isoforms in skeletal muscle.
- Development of new technologies, based on Inductively Coupled Plasma-Mass Spectroscopy (ICP-MS) for measuring ion and nutrient transport in biological samples, including cultured cells, skeletal muscle single fibers and intact muscles.
- Klf2, in the inflammatory response to injury
Our current research focuses on the question: How do contracting skeletal muscles take up glucose by a mechanism that does not require insulin? This study is motivated by the fact that contraction-induced glucose uptake, which does not require insulin, can exceed insulin-dependent glucose uptake by 50- to 100-fold and, therefore, is critical for maintaining glucose homeostasis in disorders of insulin-dependent glucose uptake (diabetes). These studies represent an ongoing collaboration between my laboratory and the laboratory of Dr. Julio Landero-Figuero, Dept. of Chemistry, Univ. of Cincinnati.
We use a range of experimental approaches to address this question, including: in vitro measurement of glucose uptake in isolated contracting mouse skeletal muscles of WT and TG mice; simultaneous measurement of multiple ions (K/Rb, Ca, Na, et al.) and carbon-based molecules (13C-glucose et al.) that are potentially co-transported during contraction; and immunohistochemistry assays and functional measurements of muscle force in situ and in vivo.
Contact UsDepartment of
Pharmacology and Systems Physiology
College of Medicine
231 Albert Sabin Way
Cincinnati, OH 45267-0575