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Pharmacology & Systems Physiology 

Photo of John Lorenz, PhD

John Lorenz, PhD

Professor

Education/Credentials
  • B.S.: Thomas More College
  • Ph.D.: University of Kentucky

Research/Clinical Interests

The research activities in this lab are focused on the study of integrative physiology in a variety of genetically altered mice produced at UC and elsewhere. Recognizing that functional techniques must be made available in order to thoroughly evaluate the physiologic and integrative role of regulatory proteins using transgenic approaches, we have developed a facility that is able to perform in depth analyses of cardiovascular, renal, and pulmonary systems in the intact mouse. Methodologies include 1) evaluation of myocardial function in vivo using cardiac catheterization (pressure and volume measurements) and echocardiography, as well as in vitro using the Langendorff preparation (PowerPoint presentation illustrating methods used to evaluate cardiac function); 2) evaluation of renal function in anesthetized animals using clearance measurements of glomerular filtration rate, renal blood flow and electrolyte excretion; and 3) evaluation of pulmonary mechanics in both instrumented (pressure-flow measurements) and non-instrumented animals (plethysmography). In addition to these activities, my lab is specifically engaged in research designed to evaluate the role of various ion transporters in the control of blood pressure and renal function. Using molecular techniques to produce mice lacking various kidney-specific transporters, we are studying the contribution of these transporters to overall fluid and electrolyte homeostasis. Presently, we are pursuing the phenotypic analysis of several knockout strains of mice including the NHE3, NHE2, ROMK, colonic H-K-ATPase, and NKCC1 knockouts. In addition, we have recently begun to investigate the mechanisms involved in balancing salt intake with urinary salt excretion. According to our hypothesis, this reflex, referred to as the anticipatory salt reflex, or the hepatorenal reflex, may involve the gastrointestinal peptides guanylin and uroguanylin as paracrine/autocrine mediators of a hepatorenal neural reflex. Using sophisticated techniques such as in vivo renal micropuncture and microperfusion, and renal nerve recordings, we hope to gain a more thorough understanding of the salt and fluid homeostatic mechanisms within the kidney. Ultimately, these studies investigating hemodynamics and fluid and electrolyte balance will provide insights to genetic basis of disease states such as hypertension.

Contact Information

Peer Reviewed Publications

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Prasad, Vikram; Lorenz, John N; Lasko, Valerie M; Nieman, Michelle L; Huang, Wei; Wang, Yigang; Wieczorek, David W; Shull, Gary E 2015. SERCA2 Haploinsufficiency in a Mouse Model of Darier Disease Causes a Selective Predisposition to Heart Failure. BioMed research international, 2015 , 251598

Prasad, Vikram; Lorenz, John N; Lasko, Valerie M; Nieman, Michelle L; Jiang, Min; Gao, Xu; Rubinstein, Jack; Wieczorek, David F; Shull, Gary E 2014. Ablation of plasma membrane Ca(2+)-ATPase isoform 4 prevents development of hypertrophy in a model of hypertrophic cardiomyopathy. Journal of molecular and cellular cardiology, 77 , 53-63

Rubinstein, Jack; Lasko, Valerie M; Koch, Sheryl E; Singh, Vivek P; Carreira, Vinicius; Robbins, Nathan; Patel, Amit R; Jiang, Min; Bidwell, Philip; Kranias, Evangelia G; Jones, W Keith; Lorenz, John N 2014. Novel role of transient receptor potential vanilloid 2 in the regulation of cardiac performance. American journal of physiology. Heart and circulatory physiology, 306 4, H574-84

Auger-Messier, Mannix; Accornero, Federica; Goonasekera, Sanjeewa A; Bueno, Orlando F; Lorenz, John N; van Berlo, Jop H; Willette, Robert N; Molkentin, Jeffery D 2013. Unrestrained p38 MAPK activation in Dusp1/4 double-null mice induces cardiomyopathy. Circulation research, 112 1, 48-56

Gupta, Manish K; Gulick, James; James, Jeanne; Osinska, Hanna; Lorenz, John N; Robbins, Jeffrey 2013. Functional dissection of myosin binding protein C phosphorylation. Journal of molecular and cellular cardiology, 64 , 39-50

Prasad, Vikram; Lorenz, John N; Lasko, Valerie M; Nieman, Michelle L; Al Moamen, Nabeel J; Shull, Gary E 2013. Loss of the AE3 Cl(-)/HCO(-) 3 exchanger in mice affects rate-dependent inotropy and stress-related AKT signaling in heart. Frontiers in physiology, 4 , 399

Prasad, Vikram; Lorenz, John N; Miller, Marian L; Vairamani, Kanimozhi; Nieman, Michelle L; Wang, Yigang; Shull, Gary E 2013. Loss of NHE1 activity leads to reduced oxidative stress in heart and mitigates high-fat diet-induced myocardial stress. Journal of molecular and cellular cardiology, 65 , 33-42

Rindler, Tara N; Lasko, Valerie M; Nieman, Michelle L; Okada, Motoi; Lorenz, John N; Lingrel, Jerry B 2013. Knockout of the Na,K-ATPase ?2-isoform in cardiac myocytes delays pressure overload-induced cardiac dysfunction. American journal of physiology. Heart and circulatory physiology, 304 8, H1147-58

Singh, Vivek P; Rubinstein, Jack; Arvanitis, Demetrios A; Ren, Xiaoping; Gao, Xiaoqian; Haghighi, Kobra; Gilbert, Mark; Iyer, Venkat R; Kim, Do Han; Cho, Chunghee; Jones, Keith; Lorenz, John N; Armstrong, Clara F; Wang, Hong-Sheng; Gyorke, Sandor; Kranias, Evangelia G 2013. Abnormal calcium cycling and cardiac arrhythmias associated with the human Ser96Ala genetic variant of histidine-rich calcium-binding protein. Journal of the American Heart Association, 2 5, e000460

Koch, Sheryl E; Gao, Xiaoqian; Haar, Lauren; Jiang, Min; Lasko, Valerie M; Robbins, Nathan; Cai, Wenfeng; Brokamp, Cole; Varma, Priyanka; Tranter, Michael; Liu, Yong; Ren, Xiaoping; Lorenz, John N; Wang, Hong-Sheng; Jones, W Keith; Rubinstein, Jack 2012. Probenecid: novel use as a non-injurious positive inotrope acting via cardiac TRPV2 stimulation. Journal of molecular and cellular cardiology, 53 1, 134-44

Lorenz, John N 2012. Micropuncture of the kidney: a primer on techniques. Comprehensive Physiology, 2 1, 621-37

Lorenz, John N; Oshiro, Naomi; Loreaux, Elizabeth L; Lingrel, Jerry B 2012. DOCA-salt hypertension does not require the ouabain-sensitive binding site of the ?2 Na,K-ATPase. American journal of hypertension, 25 4, 421-9

Lynch, Jeffrey M; Maillet, Marjorie; Vanhoutte, Davy; Schloemer, Aryn; Sargent, Michelle A; Blair, N Scott; Lynch, Kaari A; Okada, Tetsuya; Aronow, Bruce J; Osinska, Hanna; Prywes, Ron; Lorenz, John N; Mori, Kazutoshi; Lawler, Jack; Robbins, Jeffrey; Molkentin, Jeffery D 2012. A thrombospondin-dependent pathway for a protective ER stress response. Cell, 149 6, 1257-68

Accornero, Federica; van Berlo, Jop H; Benard, Matthew J; Lorenz, John N; Carmeliet, Peter; Molkentin, Jeffery D 2011. Placental growth factor regulates cardiac adaptation and hypertrophy through a paracrine mechanism. Circulation research, 109 3, 272-80

Al Moamen, Nabeel J; Prasad, Vikram; Bodi, Ilona; Miller, Marian L; Neiman, Michelle L; Lasko, Valerie M; Alper, Seth L; Wieczorek, David F; Lorenz, John N; Shull, Gary E 2011. Loss of the AE3 anion exchanger in a hypertrophic cardiomyopathy model causes rapid decompensation and heart failure. Journal of molecular and cellular cardiology, 50 1, 137-46

Kehat, Izhak; Davis, Jennifer; Tiburcy, Malte; Accornero, Federica; Saba-El-Leil, Marc K; Maillet, Marjorie; York, Allen J; Lorenz, John N; Zimmermann, Wolfram H; Meloche, Sylvain; Molkentin, Jeffery D 2011. Extracellular signal-regulated kinases 1 and 2 regulate the balance between eccentric and concentric cardiac growth. Circulation research, 108 2, 176-83

Lorenz, John N; Lasko, Valerie M; Nieman, Michelle L; Damhoff, Thomas; Prasad, Vikram; Beierwaltes, William H; Lingrel, Jerry B 2011. Renovascular hypertension using a modified two-kidney, one-clip approach in mice is not dependent on the ?1 or ?2 Na-K-ATPase ouabain-binding site. American journal of physiology. Renal physiology, 301 3, F615-21

Rindler, Tara N; Dostanic, Iva; Lasko, Valerie M; Nieman, Michelle L; Neumann, Jonathan C; Lorenz, John N; Lingrel, Jerry B 2011. Knockout of the Na,K-ATPase ??-isoform in the cardiovascular system does not alter basal blood pressure but prevents ACTH-induced hypertension. American journal of physiology. Heart and circulatory physiology, 301 4, H1396-404

Wansapura, Arshani N; Lasko, Valerie M; Lingrel, Jerry B; Lorenz, John N 2011. Mice expressing ouabain-sensitive ?1-Na,K-ATPase have increased susceptibility to pressure overload-induced cardiac hypertrophy. American journal of physiology. Heart and circulatory physiology, 300 1, H347-55

Bradford, Emily M; Miller, Marian L; Prasad, Vikram; Nieman, Michelle L; Gawenis, Lara R; Berryman, Mark; Lorenz, John N; Tso, Patrick; Shull, Gary E 2010. CLIC5 mutant mice are resistant to diet-induced obesity and exhibit gastric hemorrhaging and increased susceptibility to torpor. American journal of physiology. Regulatory, integrative and comparative physiology, 298 6, R1531-42

Maillet, Marjorie; Davis, Jennifer; Auger-Messier, Mannix; York, Allen; Osinska, Hanna; Piquereau, Jérôme; Lorenz, John N; Robbins, Jeffrey; Ventura-Clapier, Renée; Molkentin, Jeffery D 2010. Heart-specific deletion of CnB1 reveals multiple mechanisms whereby calcineurin regulates cardiac growth and function. The Journal of biological chemistry, 285 9, 6716-24

Nakayama, Hiroyuki; Bodi, Ilona; Maillet, Marjorie; DeSantiago, Jaime; Domeier, Timothy L; Mikoshiba, Katsuhiko; Lorenz, John N; Blatter, Lothar A; Bers, Donald M; Molkentin, Jeffery D 2010. The IP3 receptor regulates cardiac hypertrophy in response to select stimuli. Circulation research, 107 5, 659-66

Liu, Qinghang; Chen, Xiongwen; Macdonnell, Scott M; Kranias, Evangelia G; Lorenz, John N; Leitges, Michael; Houser, Steven R; Molkentin, Jeffery D 2009. Protein kinase C{alpha}, but not PKC{beta} or PKC{gamma}, regulates contractility and heart failure susceptibility: implications for ruboxistaurin as a novel therapeutic approach. Circulation research, 105 2, 194-200

Maloyan, Alina; Osinska, Hanna; Lammerding, Jan; Lee, Richard T; Cingolani, Oscar H; Kass, David A; Lorenz, John N; Robbins, Jeffrey 2009. Biochemical and mechanical dysfunction in a mouse model of desmin-related myopathy. Circulation research, 104 8, 1021-8

Sadayappan, Sakthivel; Gulick, James; Klevitsky, Raisa; Lorenz, John N; Sargent, Michelle; Molkentin, Jeffery D; Robbins, Jeffrey 2009. Cardiac myosin binding protein-C phosphorylation in a {beta}-myosin heavy chain background. Circulation, 119 9, 1253-62

Wansapura, Arshani N; Lasko, Valerie; Xie, Zijian; Fedorova, Olga V; Bagrov, Alexei Y; Lingrel, Jerry B; Lorenz, John N 2009. Marinobufagenin enhances cardiac contractility in mice with ouabain-sensitive alpha1 Na+-K+-ATPase. American journal of physiology. Heart and circulatory physiology, 296 6, H1833-9

Diwan, Abhinav; Wansapura, Janaka; Syed, Faisal M; Matkovich, Scot J; Lorenz, John N; Dorn, Gerald W 2008. Nix-mediated apoptosis links myocardial fibrosis, cardiac remodeling, and hypertrophy decompensation. Circulation, 117 3, 396-404

Loreaux, Elizabeth L; Kaul, Baksho; Lorenz, John N; Lingrel, Jerry B 2008. Ouabain-Sensitive alpha1 Na,K-ATPase enhances natriuretic response to saline load. Journal of the American Society of Nephrology : JASN, 19 10, 1947-54

Lorenz, John N; Loreaux, Elizabeth L; Dostanic-Larson, Iva; Lasko, Valerie; Schnetzer, J Renee; Paul, Richard J; Lingrel, Jerry B 2008. ACTH-induced hypertension is dependent on the ouabain-binding site of the alpha2-Na+-K+-ATPase subunit. American journal of physiology. Heart and circulatory physiology, 295 1, H273-80

Prasad, Vikram; Bodi, Ilona; Meyer, Jamie W; Wang, Yigang; Ashraf, Muhammad; Engle, Sandra J; Doetschman, Thomas; Sisco, Karena; Nieman, Michelle L; Miller, Marian L; Lorenz, John N; Shull, Gary E 2008. Impaired cardiac contractility in mice lacking both the AE3 Cl-/HCO3- exchanger and the NKCC1 Na+-K+-2Cl- cotransporter: effects on Ca2+ handling and protein phosphatases. The Journal of biological chemistry, 283 46, 31303-14

Sadayappan, Sakthivel; Finley, Natosha; Howarth, Jack W; Osinska, Hanna; Klevitsky, Raisa; Lorenz, John N; Rosevear, Paul R; Robbins, Jeffrey 2008. Role of the acidic N' region of cardiac troponin I in regulating myocardial function. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 22 4, 1246-57

Gawenis, Lara R; Bradford, Emily M; Prasad, Vikram; Lorenz, John N; Simpson, Janet E; Clarke, Lane L; Woo, Alison L; Grisham, Christina; Sanford, L Philip; Doetschman, Thomas; Miller, Marian L; Shull, Gary E 2007. Colonic anion secretory defects and metabolic acidosis in mice lacking the NBC1 Na+/HCO3- cotransporter. The Journal of biological chemistry, 282 12, 9042-52

Hambleton, Michael; York, Allen; Sargent, Michelle A; Kaiser, Robert A; Lorenz, John N; Robbins, Jeffrey; Molkentin, Jeffery D 2007. Inducible and myocyte-specific inhibition of PKCalpha enhances cardiac contractility and protects against infarction-induced heart failure. American journal of physiology. Heart and circulatory physiology, 293 6, H3768-71

Kawedia, Jitesh D; Nieman, Michelle L; Boivin, Gregory P; Melvin, James E; Kikuchi, Ken-Ichiro; Hand, Arthur R; Lorenz, John N; Menon, Anil G 2007. Interaction between transcellular and paracellular water transport pathways through Aquaporin 5 and the tight junction complex. Proceedings of the National Academy of Sciences of the United States of America, 104 9, 3621-6

Lorenz, John N; Arend, Lois J; Robitz, Rachel; Paul, Richard J; MacLennan, A John 2007. Vascular dysfunction in S1P2 sphingosine 1-phosphate receptor knockout mice. American journal of physiology. Regulatory, integrative and comparative physiology, 292 1, R440-6

Pritchard, Tracy J; Parvatiyar, Michelle; Bullard, Daniel P; Lynch, Ronald M; Lorenz, John N; Paul, Richard J 2007. Transgenic mice expressing Na+-K+-ATPase in smooth muscle decreases blood pressure. American journal of physiology. Heart and circulatory physiology, 293 2, H1172-82

Adhami, Faisal; Liao, Guanghong; Morozov, Yury M; Schloemer, Aryn; Schmithorst, Vincent J; Lorenz, John N; Dunn, R Scott; Vorhees, Charles V; Wills-Karp, Marsha; Degen, Jay L; Davis, Roger J; Mizushima, Noboru; Rakic, Pasko; Dardzinski, Bernard J; Holland, Scott K; Sharp, Frank R; Kuan, Chia-Yi 2006. Cerebral ischemia-hypoxia induces intravascular coagulation and autophagy. The American journal of pathology, 169 2, 566-83

Dostanic-Larson, Iva; Lorenz, John N; Van Huysse, James W; Neumann, Jon C; Moseley, Amy E; Lingrel, Jerry B 2006. Physiological role of the alpha1- and alpha2-isoforms of the Na+-K+-ATPase and biological significance of their cardiac glycoside binding site. American journal of physiology. Regulatory, integrative and comparative physiology, 290 3, R524-8

Elitsur, Noeet; Lorenz, John N; Hawkins, Jennifer A; Rudolph, Jeffrey A; Witte, David; Yang, Li E; McDonough, Alicia A; Cohen, Mitchell B 2006. The proximal convoluted tubule is a target for the uroguanylin-regulated natriuretic response. Journal of pediatric gastroenterology and nutrition, 43 Suppl 1 , S74-81

Lorenz, John N; Dostanic-Larson, Iva; Shull, Gary E; Lingrel, Jerry B 2006. Ouabain inhibits tubuloglomerular feedback in mutant mice with ouabain-sensitive alpha1 Na,K-ATPase. Journal of the American Society of Nephrology : JASN, 17 9, 2457-63

Sadayappan, Sakthivel; Osinska, Hanna; Klevitsky, Raisa; Lorenz, John N; Sargent, Michelle; Molkentin, Jeffrey D; Seidman, Christine E; Seidman, Jonathan G; Robbins, Jeffrey 2006. Cardiac myosin binding protein C phosphorylation is cardioprotective. Proceedings of the National Academy of Sciences of the United States of America, 103 45, 16918-23

Xu, Jian; Kimball, Thomas R; Lorenz, John N; Brown, David A; Bauskin, Asne R; Klevitsky, Raisa; Hewett, Timothy E; Breit, Samuel N; Molkentin, Jeffery D 2006. GDF15/MIC-1 functions as a protective and antihypertrophic factor released from the myocardium in association with SMAD protein activation. Circulation research, 98 3, 342-50

Dostanic, Iva; Paul, Richard J; Lorenz, John N; Theriault, Steven; Van Huysse, James W; Lingrel, Jerry B 2005. The alpha2-isoform of Na-K-ATPase mediates ouabain-induced hypertension in mice and increased vascular contractility in vitro. American journal of physiology. Heart and circulatory physiology, 288 2, H477-85

Dostanic-Larson, Iva; Van Huysse, James W; Lorenz, John N; Lingrel, Jerry B 2005. The highly conserved cardiac glycoside binding site of Na,K-ATPase plays a role in blood pressure regulation. Proceedings of the National Academy of Sciences of the United States of America, 102 44, 15845-50

Noonan, William T; Woo, Alison L; Nieman, Michelle L; Prasad, Vikram; Schultheis, Patrick J; Shull, Gary E; Lorenz, John N 2005. Blood pressure maintenance in NHE3-deficient mice with transgenic expression of NHE3 in small intestine. American journal of physiology. Regulatory, integrative and comparative physiology, 288 3, R685-91

Pathak, Anand; del Monte, Federica; Zhao, Wen; Schultz, Jo-El; Lorenz, John N; Bodi, Ilona; Weiser, Doug; Hahn, Harvey; Carr, Andrew N; Syed, Faisal; Mavila, Nirmala; Jha, Leena; Qian, Jiang; Marreez, Yehia; Chen, Guoli; McGraw, Dennis W; Heist, E Kevin; Guerrero, J Luis; DePaoli-Roach, Anna A; Hajjar, Roger J; Kranias, Evangelia G 2005. Enhancement of cardiac function and suppression of heart failure progression by inhibition of protein phosphatase 1. Circulation research, 96 7, 756-66

Sakthivel, Sadayappan; Finley, Natosha L; Rosevear, Paul R; Lorenz, John N; Gulick, James; Kim, Scott; VanBuren, Peter; Martin, Lisa A; Robbins, Jeffrey 2005. In vivo and in vitro analysis of cardiac troponin I phosphorylation. The Journal of biological chemistry, 280 1, 703-14

Wang, Zhaohui; Wang, Tong; Petrovic, Snezana; Tuo, Biguang; Riederer, Brigitte; Barone, Sharon; Lorenz, John N; Seidler, Ursula; Aronson, Peter S; Soleimani, Manoocher 2005. Renal and intestinal transport defects in Slc26a6-null mice. American journal of physiology. Cell physiology, 288 4, C957-65

Braz, Julian C; Gregory, Kimberly; Pathak, Anand; Zhao, Wen; Sahin, Bogachan; Klevitsky, Raisa; Kimball, Thomas F; Lorenz, John N; Nairn, Angus C; Liggett, Stephen B; Bodi, Ilona; Wang, Su; Schwartz, Arnold; Lakatta, Edward G; DePaoli-Roach, Anna A; Robbins, Jeffrey; Hewett, Timothy E; Bibb, James A; Westfall, Margaret V; Kranias, Evangelia G; Molkentin, Jeffery D 2004. PKC-alpha regulates cardiac contractility and propensity toward heart failure. Nature medicine, 10 3, 248-54

Dostanic, Iva; Schultz, Jo El J; Lorenz, John N; Lingrel, Jerry B 2004. The alpha 1 isoform of Na,K-ATPase regulates cardiac contractility and functionally interacts and co-localizes with the Na/Ca exchanger in heart. The Journal of biological chemistry, 279 52, 54053-61

Kumar, A; Crawford, K; Flick, R; Klevitsky, R; Lorenz, J N; Bove, K E; Robbins, J; Lessard, J L 2004. Transgenic overexpression of cardiac actin in the mouse heart suggests coregulation of cardiac, skeletal and vascular actin expression. Transgenic research, 13 6, 531-40

Quinlan, John G; Hahn, Harvey S; Wong, Brenda L; Lorenz, John N; Wenisch, Alexandra S; Levin, Linda S 2004. Evolution of the mdx mouse cardiomyopathy: physiological and morphological findings. Neuromuscular disorders : NMD, 14 8-9, 491-6

Dostanic, Iva; Lorenz, John N; Schultz, Jo El J; Grupp, Ingrid L; Neumann, Jonathan C; Wani, Maqsood A; Lingrel, Jerry B 2003. The alpha2 isoform of Na,K-ATPase mediates ouabain-induced cardiac inotropy in mice. The Journal of biological chemistry, 278 52, 53026-34

Ji, Yong; Li, Bailing; Reed, Thomas D; Lorenz, John N; Kaetzel, Marcia A; Dedman, John R 2003. Targeted inhibition of Ca2+/calmodulin-dependent protein kinase II in cardiac longitudinal sarcoplasmic reticulum results in decreased phospholamban phosphorylation at threonine 17. The Journal of biological chemistry, 278 27, 25063-71

Krenz, Maike; Sanbe, Atsushi; Bouyer-Dalloz, Florence; Gulick, James; Klevitsky, Raisa; Hewett, Timothy E; Osinska, Hanna E; Lorenz, John N; Brosseau, Christine; Federico, Andrea; Alpert, Norman R; Warshaw, David M; Perryman, M Benjamin; Helmke, Steve M; Robbins, Jeffrey 2003. Analysis of myosin heavy chain functionality in the heart. The Journal of biological chemistry, 278 19, 17466-74

Lorenz, John N; Nieman, Michelle; Sabo, Jenine; Sanford, L Philip; Hawkins, Jennifer A; Elitsur, Noeet; Gawenis, Lara R; Clarke, Lane L; Cohen, Mitchell B 2003. Uroguanylin knockout mice have increased blood pressure and impaired natriuretic response to enteral NaCl load. The Journal of clinical investigation, 112 8, 1244-54

Noonan, William T; Lorenz, John N 2003. Clearance studies in genetically altered mice. Methods in molecular medicine, 86 , 315-27

Noonan, William T; Qian, Jin; Stuart, William D; Clemens, Thomas L; Lorenz, John N 2003. Altered renal hemodynamics in mice overexpressing the parathyroid hormone (PTH)/PTH-related peptide type 1 receptor in smooth muscle. Endocrinology, 144 11, 4931-8

Shull, Gary E; Okunade, Gbolahan; Liu, Lynne H; Kozel, Peter; Periasamy, Muthu; Lorenz, John N; Prasad, Vikram 2003. Physiological functions of plasma membrane and intracellular Ca2+ pumps revealed by analysis of null mutants. Annals of the New York Academy of Sciences, 986 , 453-60

Woo, Alison L; Noonan, William T; Schultheis, Patrick J; Neumann, Jonathan C; Manning, Patrice A; Lorenz, John N; Shull, Gary E 2003. Renal function in NHE3-deficient mice with transgenic rescue of small intestinal absorptive defect. American journal of physiology. Renal physiology, 284 6, F1190-8

Bueno, Orlando F; Wilkins, Benjamin J; Tymitz, Kevin M; Glascock, Betty J; Kimball, Thomas F; Lorenz, John N; Molkentin, Jeffery D 2002. Impaired cardiac hypertrophic response in Calcineurin Abeta -deficient mice. Proceedings of the National Academy of Sciences of the United States of America, 99 7, 4586-91

Lorenz, John N 2002. A practical guide to evaluating cardiovascular, renal, and pulmonary function in mice. American journal of physiology. Regulatory, integrative and comparative physiology, 282 6, R1565-82

Lorenz, John N; Baird, Nancy R; Judd, Louise M; Noonan, William T; Andringa, Anastasia; Doetschman, Thomas; Manning, Patrice A; Liu, Lynne H; Miller, Marian L; Shull, Gary E 2002. Impaired renal NaCl absorption in mice lacking the ROMK potassium channel, a model for type II Bartter's syndrome. The Journal of biological chemistry, 277 40, 37871-80

Meyer, Jamie W; Flagella, Michael; Sutliff, Roy L; Lorenz, John N; Nieman, Michelle L; Weber, Craig S; Paul, Richard J; Shull, Gary E 2002. Decreased blood pressure and vascular smooth muscle tone in mice lacking basolateral Na(+)-K(+)-2Cl(-) cotransporter. American journal of physiology. Heart and circulatory physiology, 283 5, H1846-55

Pieples, Kathy; Arteaga, Grace; Solaro, R John; Grupp, Ingrid; Lorenz, John N; Boivin, Greg P; Jagatheesan, Ganapathy; Labitzke, Erin; DeTombe, Pieter P; Konhilas, John P; Irving, Thomas C; Wieczorek, David F 2002. Tropomyosin 3 expression leads to hypercontractility and attenuates myofilament length-dependent Ca(2+) activation. American journal of physiology. Heart and circulatory physiology, 283 4, H1344-53

Schmidt, Albrecht G; Zhai, Jing; Carr, Andrew N; Gerst, Mike J; Lorenz, John N; Pollesello, Piero; Annila, Arto; Hoit, Brian D; Kranias, Evangelia G 2002. Structural and functional implications of the phospholamban hinge domain: impaired SR Ca2+ uptake as a primary cause of heart failure. Cardiovascular research, 56 2, 248-59

Yussman, Martin G; Toyokawa, Tsuyoshi; Odley, Amy; Lynch, Roy A; Wu, Guangyu; Colbert, Melissa C; Aronow, Bruce J; Lorenz, John N; Dorn, Gerald W 2002. Mitochondrial death protein Nix is induced in cardiac hypertrophy and triggers apoptotic cardiomyopathy. Nature medicine, 8 7, 725-30

Dalton, T P; Kerzee, J K; Wang, B; Miller, M; Dieter, M Z; Lorenz, J N; Shertzer, H G; Nerbert, D W; Puga, A 2001. Dioxin exposure is an environmental risk factor for ischemic heart disease. Cardiovascular toxicology, 1 4, 285-98

Fortner, C N; Lorenz, J N; Paul, R J 2001. Chloride channel function is linked to epithelium-dependent airway relaxation. American journal of physiology. Lung cellular and molecular physiology, 280 2, L334-41

Krane, C M; Fortner, C N; Hand, A R; McGraw, D W; Lorenz, J N; Wert, S E; Towne, J E; Paul, R J; Whitsett, J A; Menon, A G 2001. Aquaporin 5-deficient mouse lungs are hyperresponsive to cholinergic stimulation. Proceedings of the National Academy of Sciences of the United States of America, 98 24, 14114-9

Ledoussal, C; Lorenz, J N; Nieman, M L; Soleimani, M; Schultheis, P J; Shull, G E 2001. Renal salt wasting in mice lacking NHE3 Na+/H+ exchanger but not in mice lacking NHE2. American journal of physiology. Renal physiology, 281 4, F718-27

Lorenz, J N 2001. Considerations for the evaluation of renal function in genetically engineered mice. Current opinion in nephrology and hypertension, 10 1, 65-9

Wang, X; Osinska, H; Dorn, G W; Nieman, M; Lorenz, J N; Gerdes, A M; Witt, S; Kimball, T; Gulick, J; Robbins, J 2001. Mouse model of desmin-related cardiomyopathy. Circulation, 103 19, 2402-7

Liggett, S B; Tepe, N M; Lorenz, J N; Canning, A M; Jantz, T D; Mitarai, S; Yatani, A; Dorn, G W 2000. Early and delayed consequences of beta(2)-adrenergic receptor overexpression in mouse hearts: critical role for expression level. Circulation, 101 14, 1707-14

Dorn, G W; Souroujon, M C; Liron, T; Chen, C H; Gray, M O; Zhou, H Z; Csukai, M; Wu, G; Lorenz, J N; Mochly-Rosen, D 1999. Sustained in vivo cardiac protection by a rationally designed peptide that causes epsilon protein kinase C translocation. Proceedings of the National Academy of Sciences of the United States of America, 96 22, 12798-803

Dorn, G W; Tepe, N M; Lorenz, J N; Koch, W J; Liggett, S B 1999. Low- and high-level transgenic expression of beta2-adrenergic receptors differentially affect cardiac hypertrophy and function in Galphaq-overexpressing mice. Proceedings of the National Academy of Sciences of the United States of America, 96 11, 6400-5

Flagella, M; Clarke, L L; Miller, M L; Erway, L C; Giannella, R A; Andringa, A; Gawenis, L R; Kramer, J; Duffy, J J; Doetschman, T; Lorenz, J N; Yamoah, E N; Cardell, E L; Shull, G E 1999. Mice lacking the basolateral Na-K-2Cl cotransporter have impaired epithelial chloride secretion and are profoundly deaf. The Journal of biological chemistry, 274 38, 26946-55

Lorenz, J N; Gruenstein, E 1999. A simple, nonradioactive method for evaluating single-nephron filtration rate using FITC-inulin. The American journal of physiology, 276 1 Pt 2, F172-7

Lorenz, J N; Schultheis, P J; Traynor, T; Shull, G E; Schnermann, J 1999. Micropuncture analysis of single-nephron function in NHE3-deficient mice. The American journal of physiology, 277 3 Pt 2, F447-53

Maeda, S; Sutliff, R L; Qian, J; Lorenz, J N; Wang, J; Tang, H; Nakayama, T; Weber, C; Witte, D; Strauch, A R; Paul, R J; Fagin, J A; Clemens, T L 1999. Targeted overexpression of parathyroid hormone-related protein (PTHrP) to vascular smooth muscle in transgenic mice lowers blood pressure and alters vascular contractility. Endocrinology, 140 4, 1815-25

Periasamy, M; Reed, T D; Liu, L H; Ji, Y; Loukianov, E; Paul, R J; Nieman, M L; Riddle, T; Duffy, J J; Doetschman, T; Lorenz, J N; Shull, G E 1999. Impaired cardiac performance in heterozygous mice with a null mutation in the sarco(endo)plasmic reticulum Ca2+-ATPase isoform 2 (SERCA2) gene. The Journal of biological chemistry, 274 4, 2556-62

Qian, J; Lorenz, J N; Maeda, S; Sutliff, R L; Weber, C; Nakayama, T; Colbert, M C; Paul, R J; Fagin, J A; Clemens, T L 1999. Reduced blood pressure and increased sensitivity of the vasculature to parathyroid hormone-related protein (PTHrP) in transgenic mice overexpressing the PTH/PTHrP receptor in vascular smooth muscle. Endocrinology, 140 4, 1826-33

Schultz, J E; Witt, S A; Nieman, M L; Reiser, P J; Engle, S J; Zhou, M; Pawlowski, S A; Lorenz, J N; Kimball, T R; Doetschman, T 1999. Fibroblast growth factor-2 mediates pressure-induced hypertrophic response. The Journal of clinical investigation, 104 6, 709-19

Tepe, N M; Lorenz, J N; Yatani, A; Dash, R; Kranias, E G; Dorn, G W; Liggett, S B 1999. Altering the receptor-effector ratio by transgenic overexpression of type V adenylyl cyclase: enhanced basal catalytic activity and function without increased cardiomyocyte beta-adrenergic signalling. Biochemistry, 38 50, 16706-13

Grupp, I L; Lorenz, J N; Walsh, R A; Boivin, G P; Rindt, H 1998. Overexpression of alpha1B-adrenergic receptor induces left ventricular dysfunction in the absence of hypertrophy. The American journal of physiology, 275 4 Pt 2, H1338-50

Meneton, P; Schultheis, P J; Greeb, J; Nieman, M L; Liu, L H; Clarke, L L; Duffy, J J; Doetschman, T; Lorenz, J N; Shull, G E 1998. Increased sensitivity to K+ deprivation in colonic H,K-ATPase-deficient mice. The Journal of clinical investigation, 101 3, 536-42

Schultheis, P J; Clarke, L L; Meneton, P; Miller, M L; Soleimani, M; Gawenis, L R; Riddle, T M; Duffy, J J; Doetschman, T; Wang, T; Giebisch, G; Aronson, P S; Lorenz, J N; Shull, G E 1998. Renal and intestinal absorptive defects in mice lacking the NHE3 Na+/H+ exchanger. Nature genetics, 19 3, 282-5

Schultheis, P J; Lorenz, J N; Meneton, P; Nieman, M L; Riddle, T M; Flagella, M; Duffy, J J; Doetschman, T; Miller, M L; Shull, G E 1998. Phenotype resembling Gitelman's syndrome in mice lacking the apical Na+-Cl- cotransporter of the distal convoluted tubule. The Journal of biological chemistry, 273 44, 29150-5

Stockelman, M G; Lorenz, J N; Smith, F N; Boivin, G P; Sahota, A; Tischfield, J A; Stambrook, P J 1998. Chronic renal failure in a mouse model of human adenine phosphoribosyltransferase deficiency. The American journal of physiology, 275 1 Pt 2, F154-63

Williams, R V; Lorenz, J N; Witt, S A; Hellard, D T; Khoury, P R; Kimball, T R 1998. End-systolic stress-velocity and pressure-dimension relationships by transthoracic echocardiography in mice. The American journal of physiology, 274 5 Pt 2, H1828-35

Zhou, M; Sutliff, R L; Paul, R J; Lorenz, J N; Hoying, J B; Haudenschild, C C; Yin, M; Coffin, J D; Kong, L; Kranias, E G; Luo, W; Boivin, G P; Duffy, J J; Pawlowski, S A; Doetschman, T 1998. Fibroblast growth factor 2 control of vascular tone. Nature medicine, 4 2, 201-7

Colbert, M C; Hall, D G; Kimball, T R; Witt, S A; Lorenz, J N; Kirby, M L; Hewett, T E; Klevitsky, R; Robbins, J 1997. Cardiac compartment-specific overexpression of a modified retinoic acid receptor produces dilated cardiomyopathy and congestive heart failure in transgenic mice. The Journal of clinical investigation, 100 8, 1958-68

D'Angelo, D D; Sakata, Y; Lorenz, J N; Boivin, G P; Walsh, R A; Liggett, S B; Dorn, G W 1997. Transgenic Galphaq overexpression induces cardiac contractile failure in mice. Proceedings of the National Academy of Sciences of the United States of America, 94 15, 8121-6

Liu, L H; Paul, R J; Sutliff, R L; Miller, M L; Lorenz, J N; Pun, R Y; Duffy, J J; Doetschman, T; Kimura, Y; MacLennan, D H; Hoying, J B; Shull, G E 1997. Defective endothelium-dependent relaxation of vascular smooth muscle and endothelial cell Ca2+ signaling in mice lacking sarco(endo)plasmic reticulum Ca2+-ATPase isoform 3. The Journal of biological chemistry, 272 48, 30538-45

Lorenz, J N; Kranias, E G 1997. Regulatory effects of phospholamban on cardiac function in intact mice. The American journal of physiology, 273 6 Pt 2, H2826-31

Lorenz, J N; Paul, R J 1997. Dependence of Ca2+ channel currents on endogenous and exogenous sources of ATP in portal vein smooth muscle. The American journal of physiology, 272 2 Pt 2, H987-94

Lorenz, J N; Robbins, J 1997. Measurement of intraventricular pressure and cardiac performance in the intact closed-chest anesthetized mouse. The American journal of physiology, 272 3 Pt 2, H1137-46

Tam, E S; Ferguson, D G; Bielefeld, D R; Lorenz, J N; Cohen, R M; Pun, R Y 1997. Norepinephrine-mediated calcium signaling is altered in vascular smooth muscle of diabetic rat. Cell calcium, 21 2, 143-50

Turki, J; Lorenz, J N; Green, S A; Donnelly, E T; Jacinto, M; Liggett, S B 1996. Myocardial signaling defects and impaired cardiac function of a human beta 2-adrenergic receptor polymorphism expressed in transgenic mice. Proceedings of the National Academy of Sciences of the United States of America, 93 19, 10483-8

Lorenz, J N; Bielefeld, D R; Sperelakis, N 1994. Regulation of calcium channel current in A7r5 vascular smooth muscle cells by cyclic nucleotides. The American journal of physiology, 266 6 Pt 1, C1656-63

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Jagatheesan, G., S. Rajan, E.M. Schulz, R.P. Ahmed, N. Petrashevskaya, A. Schwartz, G.P. Boivin, G.M. Arteaga, T. Wang, Y.G. Wang, M. Ashraf, S.B. Liggett, J. Lorenz, R.J. Solaro, and D.F. Wieczorek. 2009. An internal domain of -tropomyosin increases myofilament Ca2+ sensitivity. Am J Physiol Heart Circ Physiol , 297(1), H181-90


Loreaux, E.L., B. Kaul, J.N. Lorenz, and J.B. Lingrel. 2008. Ouabain-sensitive 1 Na,K-ATPase enhances natriuretic response to saline load. J Am Soc Nephrol , 19(10), 1947-1954


Lorenz, J.N., E.L. Loreaux, I. Dostanic-Larson, V. Lasko, J.R. Schnetzer, R.J. Paul, and J.B. Lingrel. 2008. ACTH-induced hypertension is dependent on the ouabain-binding site of the 2-Na+-K+-ATPase subunit. Am J Physiol Heart Circ Physiol , 295(1), H273-80


Prasad, V., I. Bodi, J.W. Meyer, Y. Wang, M. Ashraf, S.J. Engle, T. Doetschman, K. Sisco, M.L. Nieman, M.L. Miller, J.N. Lorenz, and G.E. Shull. 2008. Impaired cardiac contractility in mice lacking both the AE3 Cl-/HCO3- exchanger and the NKCC1 Na+-K+-2Cl- cotransporter: effects on Ca2+ handling and protein phosphatases. , J Biol Chem 283(46), 31303-14