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Department of

Pathology and Laboratory Medicine

Photo of  Rafeeq Habeebahmed, PhD

Rafeeq Habeebahmed, PhD

Assistant Professor

Education/Credentials
  • PhD: All India Institute of Medical Sciences
  • MS: University of Cincinnati
  • MSc: University of Madras
  • Postdoctoral Fellow: University of Cincinnati

Research/Clinical Interests

The goal of our current research is to regenerate the ischemic myocardium and towards this we employ three different approaches namely stem cell transplantation, mobilization of endogenous bone marrow stem cells and promoting proliferation of host cardiomyocytes. We are also investigating the regulation of microRNA by hypoxia inducible factor (HIF-1a) in stem cells and we aim to identify the role of Hif-1a dependent miRs in cell proliferation, angiogenesis and cardiac regeneration. Towards this aim we have generated a transgenic mouse model with mir-210 over expression. We have also identified that mir-210 is involved in angiogenesis and proliferation of adult cardiomyocytes. Our other area of interest is cellular reprogramming; we were one of the first groups to reprogram skeletal myoblasts into Induced pluripotent stem cells (IPS) and to identify tumorigenic risks associated with IPSC transplantation even in an immunocompetent host. In addition, we have successfully collaborated with other researchers from within the division and outside and have produced several peer-reviewed publications.

Contact Information
  • UC Cardiovascular Diseases Center
  • 231 Albert Sabin Way
  • Cincinnati, Ohio 45267
  • Office (513) 558-1996
  • Email puthagr@ucmail.uc.edu

Peer Reviewed Publications

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Pacelli, Settimio; Acosta, Francisca; Chakravarti, Aparna R; Samanta, Saheli G; Whitlow, Jonathan; Modaresi, Saman; Ahmed, Rafeeq P H; Rajasingh, Johnson; Paul, Arghya 2017. Nanodiamond-based injectable hydrogel for sustained growth factor release: Preparation, characterization and in vitro analysis. Acta biomaterialia, ,

Pandey, Raghav; Yang, Yunhan; Jackson, Laeia; Ahmed, Rafeeq P H 2016. MicroRNAs regulating meis1 expression and inducing cardiomyocyte proliferation. Cardiovascular regenerative medicine, 3 ,

Waters, Renae; Pacelli, Settimio; Maloney, Ryan; Medhi, Indrani; Ahmed, Rafeeq P H; Paul, Arghya 2016. Stem cell secretome-rich nanoclay hydrogel: a dual action therapy for cardiovascular regeneration. Nanoscale, 8 14, 7371-6

Konoplyannikov, Mikhail; Haider, Khawaja Husnain; Lai, Vien Khach; Ahmed, Rafeeq P H; Jiang, Shujia; Ashraf, Muhammad 2013. Activation of diverse signaling pathways by ex-vivo delivery of multiple cytokines for myocardial repair Stem cells and development, 22 2, 204-15

Buccini, Stephanie; Haider, Khawaja Husnain; Ahmed, Rafeeq P H; Jiang, Shujia; Ashraf, Muhammad 2012. Cardiac progenitors derived from reprogrammed mesenchymal stem cells contribute to angiomyogenic repair of the infarcted heart Basic research in cardiology, 107 6, 301

Durrani, Shazia; Haider, Khawaja Husnain; Ahmed, Rafeeq P H; Jiang, Shujia; Ashraf, Muhammad 2012. Cytoprotective and proangiogenic activity of ex-vivo netrin-1 transgene overexpression protects the heart against ischemia/ reperfusion injury Stem cells and development, 21 10, 1769-78

Idris, Niagara Muhammad; Ashraf, Muhammad; Ahmed, Rafeeq P H; Shujia, Jiang; Haider, Khawaja H 2012. Activation of IL-11/STAT3 pathway in preconditioned human skeletal myoblasts blocks apoptotic cascade under oxidant stress Regenerative medicine, 7 1, 47-57

Ahmed, Rafeeq P H; Ashraf, Muhammad; Buccini, Stephanie; Shujia, Jiang; Haider, Husnain Kh 2011. Cardiac tumorigenic potential of induced pluripotent stem cells in an immunocompetent host with myocardial infarction Regenerative medicine, 6 2, 171-8

Ahmed, Rafeeq P H; Haider, Husnain K; Buccini, Stephanie; Li, Longhu; Jiang, Shujia; Ashraf, Muhammad 2011. Reprogramming of skeletal myoblasts for induction of pluripotency for tumor-free cardiomyogenesis in the infarcted heart Circulation research, 109 1, 60-70

Igura, Koichi; Haider, Husnain Kh; Ahmed, Rafeeq P H; Sheriff, Sulaiman; Ashraf, Muhammad 2011. Neuropeptide y and neuropeptide y y5 receptor interaction restores impaired growth potential of aging bone marrow stromal cells Rejuvenation research, 14 4, 393-403

Afzal, Muhammad R; Haider, Husnain Kh; Idris, Niagara Muhammad; Jiang, Shujia; Ahmed, Rafeeq P H; Ashraf, Muhammad 2010. Preconditioning promotes survival and angiomyogenic potential of mesenchymal stem cells in the infarcted heart via NF-kappaB signaling. Antioxidants & redox signaling, 12 6, 693-702

Ahmed, Rafeeq P H; Haider, Khawaja Husnain; Shujia, Jiang; Afzal, Muhammad Rizwan; Ashraf, Muhammad 2010. Sonic Hedgehog gene delivery to the rodent heart promotes angiogenesis via iNOS/netrin-1/PKC pathway. PloS one, 5 1, e8576

Haider, Husnain Kh; Lee, Yun-Jung; Jiang, Shujia; Ahmed, Rafeeq P H; Ryon, Mok; Ashraf, Muhammad 2010. Phosphodiesterase inhibition with tadalafil provides longer and sustained protection of stem cells. American journal of physiology. Heart and circulatory physiology, 299 5, H1395-404

Haider, Khawaja Husnain; Buccini, Stephanie; Ahmed, Rafeeq P H; Ashraf, Muhammad 2010. De novo myocardial regeneration: advances and pitfalls Antioxidants & redox signaling, 13 12, 1867-77

Haider, Khawaja Husnain; Idris, Niagara Muhammad; Kim, Ha Won; Ahmed, Rafeeq P H; Shujia, Jiang; Ashraf, Muhammad 2010. MicroRNA-21 is a key determinant in IL-11/Stat3 anti-apoptotic signalling pathway in preconditioning of skeletal myoblasts Cardiovascular research, 88 1, 168-78

Jagatheesan, Ganapathy; Rajan, Sudarsan; Ahmed, Rafeeq P H; Petrashevskaya, Natalia; Boivin, Greg; Arteaga, Grace M; Tae, Hyun-Jin; Liggett, Stephen B; Solaro, R John; Wieczorek, David F 2010. Striated muscle tropomyosin isoforms differentially regulate cardiac performance and myofilament calcium sensitivity. Journal of muscle research and cell motility, 31 3, 227-39

Jagatheesan, Ganapathy; Rajan, Sudarsan; Schulz, Emily M; Ahmed, Rafeeq P H; Petrashevskaya, Natalia; Schwartz, Arnold; Boivin, Greg P; Arteaga, Grace M; Wang, Tao; Wang, Yi-Gang; Ashraf, Muhammad; Liggett, Stephen B; Lorenz, John; Solaro, R John; Wieczorek, David F 2009. An internal domain of beta-tropomyosin increases myofilament Ca(2+) sensitivity. American journal of physiology. Heart and circulatory physiology, 297 1, H181-90

Jiang, Shujia; Kh Haider, Husnain; Ahmed, Rafeeq P H; Idris, Niagara M; Salim, Asmat; Ashraf, Muhammad 2008. Transcriptional profiling of young and old mesenchymal stem cells in response to oxygen deprivation and reparability of the infarcted myocardium. Journal of molecular and cellular cardiology, 44 3, 582-96

Warren, Chad M; Arteaga, Grace M; Rajan, Sudarsan; Ahmed, Rafeeq P H; Wieczorek, David F; Solaro, R John 2008. Use of 2-D DIGE analysis reveals altered phosphorylation in a tropomyosin mutant (Glu54Lys) linked to dilated cardiomyopathy. Proteomics, 8 1, 100-5

Rajan, Sudarsan; Ahmed, Rafeeq P H; Jagatheesan, Ganapathy; Petrashevskaya, Natalia; Boivin, Greg P; Urboniene, Dalia; Arteaga, Grace M; Wolska, Beata M; Solaro, R John; Liggett, Stephen B; Wieczorek, David F 2007. Dilated cardiomyopathy mutant tropomyosin mice develop cardiac dysfunction with significantly decreased fractional shortening and myofilament calcium sensitivity. Circulation research, 101 2, 205-14

Rajan, Sudarsan; Williams, Sarah S; Jagatheesan, Ganapathy; Ahmed, Rafeeq P H; Fuller-Bicer, Geraldine; Schwartz, Arnold; Aronow, Bruce J; Wieczorek, David F 2006. Microarray analysis of gene expression during early stages of mild and severe cardiac hypertrophy. Physiological genomics, 27 3, 309-17

Ahmed, R P H; Biswas, Arijit; Kannan, Meganathan; Bhattacharya, Maitreyee; Geisen, Christof; Seifried, Erhard; Oldenburg, Johannes; Saxena, Renu 2005. First report of a FVII-deficient Indian patient carrying double heterozygous mutations in the FVII gene. Thrombosis research, 115 6, 535-6

Ahmed, Rafeeq P H; Ivaskevicius, Vytautas; Kannan, Meganathan; Seifried, Erhard; Oldenburg, Johannes; Saxena, Renu 2005. Identification of 32 novel mutations in the factor VIII gene in Indian patients with hemophilia A. Haematologica, 90 2, 283-4

Bhattacharya, M; Biswas, A; Ahmed, R P H; Kannan, M; Gupta, M; Mahapatra, M; Choudhry, V P; Saxena, R 2005. Clinico-hematologic profile of factor XIII-deficient patients. Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 11 4, 475-80

Gupta, P K; Ahmed, R P H; Sazawal, S; Choudhry, V P; Saxena, R 2005. Relatively high frequency of VWD types 3 and 2 in a cohort of Indian patients: the role of multimeric analysis. Journal of thrombosis and haemostasis : JTH, 3 6, 1321-2

Gupta, P K; Ahmed, Rafeeq P H; Bhattacharyya, Maitreyee; Kannan, M; Biswas, Arijit; Kalra, Veena; Saxena, R 2005. Protein C system defects in Indian children with thrombosis. Annals of hematology, 84 2, 85-8

Panigrahi, I; Ahmed, R P H; Kannan, M; Kabra, M; Deka, D; Saxena, R 2005. Cord blood analysis for prenatal diagnosis of thalassemia major and hemophilia A. Indian pediatrics, 42 6, 577-81

Saxena, Renu; Gupta, Meenal; Gupta, Sunil; Kannan, M; Ahmed, R P H; Choudhry, V P 2005. Inherited heterogenous defect in platelet aggregation selectively with ADP and epinephrine--a series of 25 cases. Indian journal of pathology & microbiology, 48 3, 345-8

Ahmed, R P H; Gupta, P K; Kannan, M; Suresh, K; Kashyap, R; Choudhry, V P; Saxena, R 2004. Hemophilia A: role of FVIIIC/vWF Ag in assisting linkage analysis for carrier detection. Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 10 2, 127-31

Bhattacharyya, Maitreyee; Makharia, Govind; Kannan, M; Ahmed, R P H; Gupta, P K; Saxena, Renu 2004. Inherited prothrombotic defects in Budd-Chiari syndrome and portal vein thrombosis: a study from North India. American journal of clinical pathology, 121 6, 844-7

Ahmed, Rafeeq P H; Gupta, Pawan K; Kannan, M; Choudhry, Ved Prakash; Saxena, Renu 2003. Factor V Leiden--the commonest molecular defect in arterial and venous thrombophilia in India. Thrombosis research, 110 1, 19-21

Kannan, M; Ahmed, Rafeeq P H; Jain, Paresh; Kumar, Rajive; Choudhry, V P; Saxena, Renu 2003. Type I Glanzmann thrombasthenia: most common subtypes in North Indians. American journal of hematology, 74 2, 139-41

Saxena, R; Batra, V V; Ahmed, R P 2001. Heparin induced thrombocytopenia: prevalence in India. American journal of hematology, 68 3, 216-7

Pandey, Raghav; Ahmed, Rafeeq P H . MicroRNAs Inducing Proliferation of Quiescent Adult Cardiomyocytes. Cardiovascular regenerative medicine, 2 1,