Today is Thursday, Mar. 23, 2017

Department of

Molecular Genetics, Biochemistry & Microbiology

Flow Cytometry Core

Overview

The Department of Molecular Genetics houses a FACSCalibur four color flow cytometer for use by researchers. The instrument is available seven days a week 24 hours per day.

The instrument is available on a first-come, first serve basis and researchers can sign up online to reserve their time on the machine.

For access to the instrument and the online calendar, please contact Dr. Miller.

The FACSCalibur is configured as following:

  • FL1 Channel - Blue Laser (488nm) and 515-545nm Emission Filter (FITC, GFP, etc.)
  • FL2 Channel - Blue Laser (488nm) and 564-601nm Emission Filter (PE, PI, RFP, etc.)
  • FL3 Channel - Blue Laser (488nm) and 670LP Emission Filter (7-AAD, PerCP, etc)
  • FL4 Channel - Red Laser (635nm) and 653-669nm Emission Filter (APC, Cy5, etc.)

It is running the BS CellQuest Pro Software.

The flow cytometer is located in CARE 2876.

Resources

Our facility does not have the capacity to handle cell sorting. If you need to sort cells or for advice on cell sorting please visit our colleagues at the Shriner's Hospital Flow Core.

Pricing

Usage rates are $20.00 per 1/2 hour (1/2 hour is the minimum charge). Bills are processed quarterly and sent to the investigator.

Contact Information

If you would like more information regarding use of the flow cytometer, please contact:

William Miller, PhD
Associate Professor
Department of Molecular Genetics
william.miller@uc.edu
513-558-0866

FacsCalibur

For more information on the flow cytometry core service center, please contact:

 

William E. Miller, PhD
Associate Professor of Molecular Genetics
Scientific Director, College of Medicine Flow Cytometry Core Facility
Phone: 513 558-0866
Email: william.miller@uc.edu