Proteomics is the large-scale analysis of protein expression patterns. Along with functional genomics, proteomics is now an established tool for investigators to better understand the molecular events underlying many biological processes.
The UC Proteomics Laboratory is fully equipped with the protein separation and mass spectrometry equipment as well as the trained personnel to perform comparative analyses of complex protein mixtures and to identify the differentially-expressed proteins.
Proteomics laboratory personnel also offer guidance to the research community regarding the feasibility of a given approach and/or advice on proteomics experimental design to enhance the probability of success. Please contact the lab director to set up a consultation.
The research expertise in the Proteomics Laboratory is divided into two subcategories - protein preparation and electrophoresis section and mass spectrometry section.
Protein Preparation and Electrophoresis Section
The protein preparation and electrophoresis section assists investigators in evaluating protein expression changes that occur in a biological system, such as during disease development and progression, stress or drug exposure, or during normal cell and tissue development. The identification of changing protein expression patterns can provide important information about pathways involved in these processes and can lead to the identification of new protein markers for the diagnosis or the treatment of diseases.
The mass spectrometry section provides the identification of differentially-expressed proteins and the characterization of protein modifications (e.g. phosphorylation, glycosylation). Furthermore various heavy isotope tagging methods (e.g. iTRAQ, SILAC) are used in the laboratory such that the mass spectrometer can be used to both identify the proteins and quantify the changes in abundance. Additional research oriented projects include methods to quantify global changes in phosphorylation from cell and tissues and mass spectrometry-based methods for enzyme assays and high-throughput inhibitor screening.
The Proteomics Laboratory provides services to investigators in the analysis and identification of complex protein mixtures using Two-Dimensional Gel Electrophoresis and Mass Spectrometry. The laboratory is equipped with state-of-the-art instrumentation including four mass spectrometers, to address the various needs of the investigators.
In addition to assisting investigators in the design of their proteomics experiments and advice on sample collection and storage, the laboratory offers the following services: